Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage
Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na<sub>2</sub>SO<sub>4</sub> was used to modulate the aliphatic glucosinolate content in different organs of <i>Eruca sativa</i>...
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2025-08-01
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| author | Eleonora Pagnotta Laura Righetti Gabriele Micheletti Carla Boga Annamaria Massafra Luisa Ugolini Lorena Malaguti Roberto Matteo Federica Nicoletti Roberto Colombo Agostino Fricano Laura Bassolino |
| author_facet | Eleonora Pagnotta Laura Righetti Gabriele Micheletti Carla Boga Annamaria Massafra Luisa Ugolini Lorena Malaguti Roberto Matteo Federica Nicoletti Roberto Colombo Agostino Fricano Laura Bassolino |
| author_sort | Eleonora Pagnotta |
| collection | DOAJ |
| description | Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na<sub>2</sub>SO<sub>4</sub> was used to modulate the aliphatic glucosinolate content in different organs of <i>Eruca sativa</i> Mill. In flowers, which accumulate the highest amount of glucosinolates, Na<sub>2</sub>SO<sub>4</sub> increased the concentration of glucoraphanin, in roots of glucoerucin and in apical leaves it doubled the amount of dimeric 4-mercaptobutyl glucosinolate. The biosynthetic gene <i>Branched-Chain Aminotransferase 4</i> was also induced in roots at the highest salt concentration, while in leaves all tested genes biosynthetic genes were downregulated or unaffected. <i>Cytochromes P450 83A1 monooxygenase</i> was downregulated at the highest salt concentration in all organs. Overall, <i>E. sativa</i> is a reliable source of glucosinolates, which can be modulated with Na<sub>2</sub>SO<sub>4</sub>. |
| format | Article |
| id | doaj-art-3d9789da2ec247ca8cdc8db8d4f81bb6 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-3d9789da2ec247ca8cdc8db8d4f81bb62025-08-20T03:36:34ZengMDPI AGApplied Sciences2076-34172025-08-011515875710.3390/app15158757Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering StageEleonora Pagnotta0Laura Righetti1Gabriele Micheletti2Carla Boga3Annamaria Massafra4Luisa Ugolini5Lorena Malaguti6Roberto Matteo7Federica Nicoletti8Roberto Colombo9Agostino Fricano10Laura Bassolino11Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyDepartment of Industrial Chemistry ‘Toso Montanari’, Alma Mater Studiorum Università di Bologna, 40136 Bologna, ItalyDepartment of Industrial Chemistry ‘Toso Montanari’, Alma Mater Studiorum Università di Bologna, 40136 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Agriculture and Environment (CREA-AA), 40128 Bologna, ItalyCouncil for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics (CREA-GB), 29017 Fiorenzuola d’Arda, ItalyCouncil for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops (CREA-CI), 40128 Bologna, ItalyGlucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na<sub>2</sub>SO<sub>4</sub> was used to modulate the aliphatic glucosinolate content in different organs of <i>Eruca sativa</i> Mill. In flowers, which accumulate the highest amount of glucosinolates, Na<sub>2</sub>SO<sub>4</sub> increased the concentration of glucoraphanin, in roots of glucoerucin and in apical leaves it doubled the amount of dimeric 4-mercaptobutyl glucosinolate. The biosynthetic gene <i>Branched-Chain Aminotransferase 4</i> was also induced in roots at the highest salt concentration, while in leaves all tested genes biosynthetic genes were downregulated or unaffected. <i>Cytochromes P450 83A1 monooxygenase</i> was downregulated at the highest salt concentration in all organs. Overall, <i>E. sativa</i> is a reliable source of glucosinolates, which can be modulated with Na<sub>2</sub>SO<sub>4</sub>.https://www.mdpi.com/2076-3417/15/15/8757rocketarugulaglucoraphaninglucoerucintranscriptional profiling |
| spellingShingle | Eleonora Pagnotta Laura Righetti Gabriele Micheletti Carla Boga Annamaria Massafra Luisa Ugolini Lorena Malaguti Roberto Matteo Federica Nicoletti Roberto Colombo Agostino Fricano Laura Bassolino Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage Applied Sciences rocket arugula glucoraphanin glucoerucin transcriptional profiling |
| title | Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage |
| title_full | Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage |
| title_fullStr | Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage |
| title_full_unstemmed | Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage |
| title_short | Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage |
| title_sort | effect of sodium sulfate treatment on the modulation of aliphatic glucosinolates in i eruca sativa i mill organs at flowering stage |
| topic | rocket arugula glucoraphanin glucoerucin transcriptional profiling |
| url | https://www.mdpi.com/2076-3417/15/15/8757 |
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