Effect of Sodium Sulfate Treatment on the Modulation of Aliphatic Glucosinolates in <i>Eruca sativa</i> Mill Organs at Flowering Stage

Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na<sub>2</sub>SO<sub>4</sub> was used to modulate the aliphatic glucosinolate content in different organs of <i>Eruca sativa</i>...

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Main Authors: Eleonora Pagnotta, Laura Righetti, Gabriele Micheletti, Carla Boga, Annamaria Massafra, Luisa Ugolini, Lorena Malaguti, Roberto Matteo, Federica Nicoletti, Roberto Colombo, Agostino Fricano, Laura Bassolino
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Applied Sciences
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Online Access:https://www.mdpi.com/2076-3417/15/15/8757
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Summary:Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na<sub>2</sub>SO<sub>4</sub> was used to modulate the aliphatic glucosinolate content in different organs of <i>Eruca sativa</i> Mill. In flowers, which accumulate the highest amount of glucosinolates, Na<sub>2</sub>SO<sub>4</sub> increased the concentration of glucoraphanin, in roots of glucoerucin and in apical leaves it doubled the amount of dimeric 4-mercaptobutyl glucosinolate. The biosynthetic gene <i>Branched-Chain Aminotransferase 4</i> was also induced in roots at the highest salt concentration, while in leaves all tested genes biosynthetic genes were downregulated or unaffected. <i>Cytochromes P450 83A1 monooxygenase</i> was downregulated at the highest salt concentration in all organs. Overall, <i>E. sativa</i> is a reliable source of glucosinolates, which can be modulated with Na<sub>2</sub>SO<sub>4</sub>.
ISSN:2076-3417