The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>)
To explore the impacts of acute ammonia nitrogen (NH<sub>3</sub>-N) stress on gill structure and the antioxidant ability of red and white muscles in juvenile yellowfin tuna (<i>Thunnus albacares</i>), this study used natural seawater as a control, establishing two experimenta...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/13/11/1357 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850217646333624320 |
|---|---|
| author | Yongyue Sun Zhengyi Fu Xuancheng Liu Zhenhua Ma |
| author_facet | Yongyue Sun Zhengyi Fu Xuancheng Liu Zhenhua Ma |
| author_sort | Yongyue Sun |
| collection | DOAJ |
| description | To explore the impacts of acute ammonia nitrogen (NH<sub>3</sub>-N) stress on gill structure and the antioxidant ability of red and white muscles in juvenile yellowfin tuna (<i>Thunnus albacares</i>), this study used natural seawater as a control, establishing two experimental NH<sub>3</sub>-N groups at 5 and 10 mg/L. Gills and red and white muscle were taken at 6, 24, and 36 h for the determination of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GHS-PX) levels, and to observe gill structure. The results indicated that, with increasing time, the MDA concentration and CAT activity in the gills of the 5 mg/L group showed a trend of first increasing and then decreasing, while SOD activity exhibited a downward trend. In the 10 mg/L group, MDA concentration showed an increasing trend, while SOD, CAT, and GSH-PX activities demonstrated a trend of first increasing and then decreasing. In the 5 mg/L group, the MDA concentration and GSH-PX activity in the red muscle showed an increasing trend. In the 10 mg/L group, MDA concentration and SOD and CAT activities exhibited a downward trend. In the 5 mg/L group, the MDA concentration and SOD activity in the white muscle showed a downward trend, while CAT activity exhibited an increasing trend. In the 10 mg/L group, MDA concentration and CAT activity demonstrated a trend of first increasing and then decreasing, while SOD activity showed a downward trend. Ammonia nitrogen can lead to necrosis and shedding of gill epithelial cells, cell vacuolation, edema, as well as proliferation, hypertrophy, and fusion of secondary lamellae. This study demonstrates that NH<sub>3</sub>-N can alter gill structure and reduce the antioxidant ability of gills and red–white muscle. The findings provide scientific data that can support the aquaculture and recirculating aquaculture systems of juvenile tuna. |
| format | Article |
| id | doaj-art-3d90be01724d40739e4139e1ecfeb66f |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-3d90be01724d40739e4139e1ecfeb66f2025-08-20T02:08:00ZengMDPI AGAntioxidants2076-39212024-11-011311135710.3390/antiox13111357The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>)Yongyue Sun0Zhengyi Fu1Xuancheng Liu2Zhenhua Ma3Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, ChinaKey Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, ChinaCollege of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028007, ChinaKey Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, ChinaTo explore the impacts of acute ammonia nitrogen (NH<sub>3</sub>-N) stress on gill structure and the antioxidant ability of red and white muscles in juvenile yellowfin tuna (<i>Thunnus albacares</i>), this study used natural seawater as a control, establishing two experimental NH<sub>3</sub>-N groups at 5 and 10 mg/L. Gills and red and white muscle were taken at 6, 24, and 36 h for the determination of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GHS-PX) levels, and to observe gill structure. The results indicated that, with increasing time, the MDA concentration and CAT activity in the gills of the 5 mg/L group showed a trend of first increasing and then decreasing, while SOD activity exhibited a downward trend. In the 10 mg/L group, MDA concentration showed an increasing trend, while SOD, CAT, and GSH-PX activities demonstrated a trend of first increasing and then decreasing. In the 5 mg/L group, the MDA concentration and GSH-PX activity in the red muscle showed an increasing trend. In the 10 mg/L group, MDA concentration and SOD and CAT activities exhibited a downward trend. In the 5 mg/L group, the MDA concentration and SOD activity in the white muscle showed a downward trend, while CAT activity exhibited an increasing trend. In the 10 mg/L group, MDA concentration and CAT activity demonstrated a trend of first increasing and then decreasing, while SOD activity showed a downward trend. Ammonia nitrogen can lead to necrosis and shedding of gill epithelial cells, cell vacuolation, edema, as well as proliferation, hypertrophy, and fusion of secondary lamellae. This study demonstrates that NH<sub>3</sub>-N can alter gill structure and reduce the antioxidant ability of gills and red–white muscle. The findings provide scientific data that can support the aquaculture and recirculating aquaculture systems of juvenile tuna.https://www.mdpi.com/2076-3921/13/11/1357NH<sub>3</sub>-Njuvenile yellowfin tunagill tissue sectionsmuscleantioxidant ability |
| spellingShingle | Yongyue Sun Zhengyi Fu Xuancheng Liu Zhenhua Ma The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) Antioxidants NH<sub>3</sub>-N juvenile yellowfin tuna gill tissue sections muscle antioxidant ability |
| title | The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) |
| title_full | The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) |
| title_fullStr | The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) |
| title_full_unstemmed | The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) |
| title_short | The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (<i>Thunnus albacares</i>) |
| title_sort | impact of acute ammonia nitrogen stress on the gill tissue structure and antioxidant ability of gills and red and white muscle in juvenile yellowfin tuna i thunnus albacares i |
| topic | NH<sub>3</sub>-N juvenile yellowfin tuna gill tissue sections muscle antioxidant ability |
| url | https://www.mdpi.com/2076-3921/13/11/1357 |
| work_keys_str_mv | AT yongyuesun theimpactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT zhengyifu theimpactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT xuanchengliu theimpactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT zhenhuama theimpactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT yongyuesun impactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT zhengyifu impactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT xuanchengliu impactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi AT zhenhuama impactofacuteammonianitrogenstressonthegilltissuestructureandantioxidantabilityofgillsandredandwhitemuscleinjuvenileyellowfintunaithunnusalbacaresi |