CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p
Osteosarcoma (OS) is a malignant bone tumour that commonly occurs in paediatric and adolescent patients. Currently, effective therapy for OS remains elusive due to poor patient survival rates. In this study, we observed significantly elevated expressions of circTUBA1C in OS tumours and cells. Si...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2024-10-01
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| Series: | Polish Journal of Pathology |
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| Online Access: | https://www.termedia.pl/CircRNA-TUBA1C-promotes-proliferation-and-glucose-metabolism-and-blocks-apoptosis-of-osteosarcoma-cells-through-sponging-miR-143-3p,55,54826,1,1.html |
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| author | Yi-Bing Liu Wen-Hao Dai Jun-Jie Chang Kang Wei |
| author_facet | Yi-Bing Liu Wen-Hao Dai Jun-Jie Chang Kang Wei |
| author_sort | Yi-Bing Liu |
| collection | DOAJ |
| description | Osteosarcoma (OS) is a malignant bone tumour that commonly occurs in paediatric and adolescent patients. Currently, effective therapy for OS remains elusive due to poor patient survival rates.
In this study, we observed significantly elevated expressions of circTUBA1C in OS tumours and cells.
Silencing circTUBA1C effectively suppressed proliferation and glucose metabolism, and promoted apoptosis of OS cells. Furthermore, we discovered that miR-143-3p played a reverse role to circTUBA1C in OS cells. Bioinformatics analysis, RNA pull-down assay, and luciferase assay demonstrated that circTUBA1C acted as a sponge for miR-143-3p, blocking its expression in OS cells. Finally, rescue experiments showed that inhibition of miR-143-3p in circTUBA1C-silenced OS cells significantly overrode the low-circTUBA1C-mediated miR-143-3p upregulation and OS cell progression in vitro and in vivo .
Our results demonstrate the critical roles and molecular targets of circTUBA1C in modulating OS progression, suggesting that circTUBA1C inhibition could serve as a new therapeutic strategy for treating OS. |
| format | Article |
| id | doaj-art-3d859a6835784fbf996c078826a63689 |
| institution | Kabale University |
| issn | 1233-9687 2084-9869 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Polish Journal of Pathology |
| spelling | doaj-art-3d859a6835784fbf996c078826a636892025-01-27T11:36:07ZengTermedia Publishing HousePolish Journal of Pathology1233-96872084-98692024-10-0175321522710.5114/pjp.2024.14323154826CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3pYi-Bing LiuWen-Hao DaiJun-Jie ChangKang WeiOsteosarcoma (OS) is a malignant bone tumour that commonly occurs in paediatric and adolescent patients. Currently, effective therapy for OS remains elusive due to poor patient survival rates. In this study, we observed significantly elevated expressions of circTUBA1C in OS tumours and cells. Silencing circTUBA1C effectively suppressed proliferation and glucose metabolism, and promoted apoptosis of OS cells. Furthermore, we discovered that miR-143-3p played a reverse role to circTUBA1C in OS cells. Bioinformatics analysis, RNA pull-down assay, and luciferase assay demonstrated that circTUBA1C acted as a sponge for miR-143-3p, blocking its expression in OS cells. Finally, rescue experiments showed that inhibition of miR-143-3p in circTUBA1C-silenced OS cells significantly overrode the low-circTUBA1C-mediated miR-143-3p upregulation and OS cell progression in vitro and in vivo . Our results demonstrate the critical roles and molecular targets of circTUBA1C in modulating OS progression, suggesting that circTUBA1C inhibition could serve as a new therapeutic strategy for treating OS.https://www.termedia.pl/CircRNA-TUBA1C-promotes-proliferation-and-glucose-metabolism-and-blocks-apoptosis-of-osteosarcoma-cells-through-sponging-miR-143-3p,55,54826,1,1.htmlosteosarcoma circular rna circtuba1c mir-143-3p cell proliferation glucose metabolism cell apoptosis. |
| spellingShingle | Yi-Bing Liu Wen-Hao Dai Jun-Jie Chang Kang Wei CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p Polish Journal of Pathology osteosarcoma circular rna circtuba1c mir-143-3p cell proliferation glucose metabolism cell apoptosis. |
| title | CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p |
| title_full | CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p |
| title_fullStr | CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p |
| title_full_unstemmed | CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p |
| title_short | CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p |
| title_sort | circrna tuba1c promotes proliferation and glucose metabolism and blocks apoptosis of osteosarcoma cells through sponging mir 143 3p |
| topic | osteosarcoma circular rna circtuba1c mir-143-3p cell proliferation glucose metabolism cell apoptosis. |
| url | https://www.termedia.pl/CircRNA-TUBA1C-promotes-proliferation-and-glucose-metabolism-and-blocks-apoptosis-of-osteosarcoma-cells-through-sponging-miR-143-3p,55,54826,1,1.html |
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