Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma
Colorectal cancer (CRC) is one of the most common gastrointestinal cancers in the world, and its incidence is increasing all over the world including China. In recent years, research data show that some miRNAs are differentially expressed in cancer tissues, and their expression is closely contribute...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/5587084 |
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| author | Junhua Li null Bulin Baila Tian Xiang Xu Jiang Song null Su Rina Ji Wu Tegexibaiyin Wang |
| author_facet | Junhua Li null Bulin Baila Tian Xiang Xu Jiang Song null Su Rina Ji Wu Tegexibaiyin Wang |
| author_sort | Junhua Li |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is one of the most common gastrointestinal cancers in the world, and its incidence is increasing all over the world including China. In recent years, research data show that some miRNAs are differentially expressed in cancer tissues, and their expression is closely contributed with the prognosis of CRC. Microarray technology was used, and 179 miRNAs were screened out with significantly altered expression in CRC tissues compared with adjacent tissues. The expression of mir-145-5p in tumor tissues was 3.48 times lower than that in normal tissues. Using bioinformatics technology and network resource prediction, we found that mir-145-5p had a potential target gene relationship with msln gene. Then, qRT-PCR was used to validate the expression level of mir-145-5p and msln mRNA in CRC and paracancerous tissues. The results showed that msln mRNA was higher than in normal tissues, while mir-145-5p was lower, with statistically significant difference (P<0.01, n=3). Furthermore, the expression of msln protein in CRC and normal colorectal tissues was detected by protein mass spectrometry (MRM) (n=3) and immunohistochemistry in a total case of 30 colorectal cancer tissues and normal tissues. Result showed that the positive expression of msln in CRC was higher than that in normal colorectal tissues, 1.38e-6 and 1.89e-6, respectively (P<0.01, n=3). Furthermore, in 48 h RTCA real-time monitoring experiment, mir-145-5p showed inhibitory effect on the proliferation of colo320 cells stimulated by msln. This study demonstrated that msln is a target gene of mir-145-5p in CRC. Besides, mir-145-5p negatively regulates the proliferation of CRC colo320 cells through downregulating msln gene expression in CRC colo320 cells. |
| format | Article |
| id | doaj-art-3d7da6257c5a40aeae04136f80da0bf0 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-3d7da6257c5a40aeae04136f80da0bf02025-08-20T03:35:51ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/5587084Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal AdenocarcinomaJunhua Li0null Bulin Baila1Tian Xiang Xu2Jiang Song3null Su Rina4Ji Wu5Tegexibaiyin Wang6PLA Research Institute of General SurgeryPharmacy LaboratoryAbdominal Tumor SurgeryAbdominal Tumor SurgeryAbdominal Tumor SurgeryPLA Research Institute of General SurgeryPharmacy LaboratoryColorectal cancer (CRC) is one of the most common gastrointestinal cancers in the world, and its incidence is increasing all over the world including China. In recent years, research data show that some miRNAs are differentially expressed in cancer tissues, and their expression is closely contributed with the prognosis of CRC. Microarray technology was used, and 179 miRNAs were screened out with significantly altered expression in CRC tissues compared with adjacent tissues. The expression of mir-145-5p in tumor tissues was 3.48 times lower than that in normal tissues. Using bioinformatics technology and network resource prediction, we found that mir-145-5p had a potential target gene relationship with msln gene. Then, qRT-PCR was used to validate the expression level of mir-145-5p and msln mRNA in CRC and paracancerous tissues. The results showed that msln mRNA was higher than in normal tissues, while mir-145-5p was lower, with statistically significant difference (P<0.01, n=3). Furthermore, the expression of msln protein in CRC and normal colorectal tissues was detected by protein mass spectrometry (MRM) (n=3) and immunohistochemistry in a total case of 30 colorectal cancer tissues and normal tissues. Result showed that the positive expression of msln in CRC was higher than that in normal colorectal tissues, 1.38e-6 and 1.89e-6, respectively (P<0.01, n=3). Furthermore, in 48 h RTCA real-time monitoring experiment, mir-145-5p showed inhibitory effect on the proliferation of colo320 cells stimulated by msln. This study demonstrated that msln is a target gene of mir-145-5p in CRC. Besides, mir-145-5p negatively regulates the proliferation of CRC colo320 cells through downregulating msln gene expression in CRC colo320 cells.http://dx.doi.org/10.1155/2022/5587084 |
| spellingShingle | Junhua Li null Bulin Baila Tian Xiang Xu Jiang Song null Su Rina Ji Wu Tegexibaiyin Wang Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma Analytical Cellular Pathology |
| title | Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma |
| title_full | Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma |
| title_fullStr | Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma |
| title_full_unstemmed | Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma |
| title_short | Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma |
| title_sort | effects of hsa mir 145 5p on the regulation of msln expression in colorectal adenocarcinoma |
| url | http://dx.doi.org/10.1155/2022/5587084 |
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