α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark
This study evaluated the inhibitory effects of different extracts and fractions from Eucalyptus. grandis × urophylla bark (EB) against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; amo...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/8516964 |
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| author | Ping Jiang Jia Xiong Fei Wang Mary H. Grace Mary Ann Lila Rui Xu |
| author_facet | Ping Jiang Jia Xiong Fei Wang Mary H. Grace Mary Ann Lila Rui Xu |
| author_sort | Ping Jiang |
| collection | DOAJ |
| description | This study evaluated the inhibitory effects of different extracts and fractions from Eucalyptus. grandis × urophylla bark (EB) against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC50 of 1.40±0.18 and 1.72±0.12 μg/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79±4.68 and 920.4±5.46 mg GAEag−1, respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3-α-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-α-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from Eucalyptus grandis × E. urograndis bark potently inhibited α-amylase and α-glucosidase activity, having potential in prevention of hyperglycemia. |
| format | Article |
| id | doaj-art-3d7464312c4a47e9a4eba84706c152e4 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-3d7464312c4a47e9a4eba84706c152e42025-02-03T01:22:55ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/85169648516964α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla BarkPing Jiang0Jia Xiong1Fei Wang2Mary H. Grace3Mary Ann Lila4Rui Xu5College of Chemical Engineering, Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu 210037, ChinaCollege of Chemical Engineering, Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu 210037, ChinaCollege of Chemical Engineering, Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu 210037, ChinaPlants for Human Health Institute, Department of Food Bioprocessing and Nutritional Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USAPlants for Human Health Institute, Department of Food Bioprocessing and Nutritional Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USACollege of Chemical Engineering, Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing, Jiangsu 210037, ChinaThis study evaluated the inhibitory effects of different extracts and fractions from Eucalyptus. grandis × urophylla bark (EB) against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC50 of 1.40±0.18 and 1.72±0.12 μg/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79±4.68 and 920.4±5.46 mg GAEag−1, respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3-α-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-α-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from Eucalyptus grandis × E. urograndis bark potently inhibited α-amylase and α-glucosidase activity, having potential in prevention of hyperglycemia.http://dx.doi.org/10.1155/2017/8516964 |
| spellingShingle | Ping Jiang Jia Xiong Fei Wang Mary H. Grace Mary Ann Lila Rui Xu α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark Journal of Chemistry |
| title | α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark |
| title_full | α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark |
| title_fullStr | α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark |
| title_full_unstemmed | α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark |
| title_short | α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis × E. urophylla Bark |
| title_sort | α amylase and α glucosidase inhibitory activities of phenolic extracts from eucalyptus grandis e urophylla bark |
| url | http://dx.doi.org/10.1155/2017/8516964 |
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