Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients
Abstract Acne vulgaris (AV) is a chronic inflammatory skin disease with a complex pathogenesis, and its association with insulin resistance (IR) remains unclear. This study aimed to identify differential proteins linking AV and IR to improve diagnostic and therapeutic strategies. Plasma proteomic pr...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-12344-5 |
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| author | Yu Rao Zheng Lei Siyu Chen Xiaoping Zhu Zongzhe Jiang Yang Xu Xia Xiong Yongmei Liao Xiqian Chen Qian Tang Zongjunlin Liu Changqiang Li |
| author_facet | Yu Rao Zheng Lei Siyu Chen Xiaoping Zhu Zongzhe Jiang Yang Xu Xia Xiong Yongmei Liao Xiqian Chen Qian Tang Zongjunlin Liu Changqiang Li |
| author_sort | Yu Rao |
| collection | DOAJ |
| description | Abstract Acne vulgaris (AV) is a chronic inflammatory skin disease with a complex pathogenesis, and its association with insulin resistance (IR) remains unclear. This study aimed to identify differential proteins linking AV and IR to improve diagnostic and therapeutic strategies. Plasma proteomic profiling by using LC-MS/MS was performed on 90 AV patients (with IR [n = 40], without IR [n = 50]) and 30 healthy controls (with IR [n = 11], without IR [n = 19]). Differentially expressed proteins were analyzed using bioinformatics tool to identify key molecules and pathways. Candidate molecules were screened based on their positive correlations with both AV severity and insulin levels. In group AV with IR, C4BPA was highly expressed, showed strong positive correlations (Pearson’s R = 0.46, p = 4.21E-6) with insulin levels and were enriched in complement agglutination cascade and B-cell-mediated immune pathways. In group AV, C4BPA (Pearson’s R = 0.23, p = 0.03) also correlated with AV severity (GAGS scores) and was enriched in complement and coagulation cascades and leukocyte-mediated immunity. C4BPA acted as a key molecule, bridging IR and AV pathogenesis. This study offers a highly valuable proteomic resource for AV associated with IR and proposes a mechanistic hypothesis, supported by existing literature, that insulin exacerbates acne by regulating lipid metabolism and inflammatory pathways with C4BPA potentially acting as a central mediator in this process. |
| format | Article |
| id | doaj-art-3d63333eab3d4bed9a75517fc2bd54c0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-3d63333eab3d4bed9a75517fc2bd54c02025-08-20T03:42:38ZengNature PortfolioScientific Reports2045-23222025-08-0115111910.1038/s41598-025-12344-5Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patientsYu Rao0Zheng Lei1Siyu Chen2Xiaoping Zhu3Zongzhe Jiang4Yang Xu5Xia Xiong6Yongmei Liao7Xiqian Chen8Qian Tang9Zongjunlin Liu10Changqiang Li11Department of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Hejiang People’s HospitalDepartment of Endocrinology and Metabolism, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityDepartment of Dermatology, The Affiliated Hospital, Southwest Medical UniversityAbstract Acne vulgaris (AV) is a chronic inflammatory skin disease with a complex pathogenesis, and its association with insulin resistance (IR) remains unclear. This study aimed to identify differential proteins linking AV and IR to improve diagnostic and therapeutic strategies. Plasma proteomic profiling by using LC-MS/MS was performed on 90 AV patients (with IR [n = 40], without IR [n = 50]) and 30 healthy controls (with IR [n = 11], without IR [n = 19]). Differentially expressed proteins were analyzed using bioinformatics tool to identify key molecules and pathways. Candidate molecules were screened based on their positive correlations with both AV severity and insulin levels. In group AV with IR, C4BPA was highly expressed, showed strong positive correlations (Pearson’s R = 0.46, p = 4.21E-6) with insulin levels and were enriched in complement agglutination cascade and B-cell-mediated immune pathways. In group AV, C4BPA (Pearson’s R = 0.23, p = 0.03) also correlated with AV severity (GAGS scores) and was enriched in complement and coagulation cascades and leukocyte-mediated immunity. C4BPA acted as a key molecule, bridging IR and AV pathogenesis. This study offers a highly valuable proteomic resource for AV associated with IR and proposes a mechanistic hypothesis, supported by existing literature, that insulin exacerbates acne by regulating lipid metabolism and inflammatory pathways with C4BPA potentially acting as a central mediator in this process.https://doi.org/10.1038/s41598-025-12344-5Acne vulgarisDifferential proteinsInsulin resistancePlasmaProteome |
| spellingShingle | Yu Rao Zheng Lei Siyu Chen Xiaoping Zhu Zongzhe Jiang Yang Xu Xia Xiong Yongmei Liao Xiqian Chen Qian Tang Zongjunlin Liu Changqiang Li Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients Scientific Reports Acne vulgaris Differential proteins Insulin resistance Plasma Proteome |
| title | Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| title_full | Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| title_fullStr | Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| title_full_unstemmed | Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| title_short | Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| title_sort | differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients |
| topic | Acne vulgaris Differential proteins Insulin resistance Plasma Proteome |
| url | https://doi.org/10.1038/s41598-025-12344-5 |
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