Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia

Abstract Aluminium (Al)-tolerant beneficial bacteria confer resistance to Al toxicity to crops in widely distributed acidic soils. However, the mechanism by which microbial consortia maintain Al tolerance under acid and Al toxicity stress remains unknown. Here, we demonstrate that a soil bacterial c...

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Main Authors: Zhiyuan Ma, Meitong Jiang, Chaoyang Liu, Ertao Wang, Yang Bai, Mengting Maggie Yuan, Shengjing Shi, Jizhong Zhou, Jixian Ding, Yimei Xie, Hui Zhang, Yan Yang, Renfang Shen, Thomas W. Crowther, Jiabao Zhang, Yuting Liang
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54616-0
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author Zhiyuan Ma
Meitong Jiang
Chaoyang Liu
Ertao Wang
Yang Bai
Mengting Maggie Yuan
Shengjing Shi
Jizhong Zhou
Jixian Ding
Yimei Xie
Hui Zhang
Yan Yang
Renfang Shen
Thomas W. Crowther
Jiabao Zhang
Yuting Liang
author_facet Zhiyuan Ma
Meitong Jiang
Chaoyang Liu
Ertao Wang
Yang Bai
Mengting Maggie Yuan
Shengjing Shi
Jizhong Zhou
Jixian Ding
Yimei Xie
Hui Zhang
Yan Yang
Renfang Shen
Thomas W. Crowther
Jiabao Zhang
Yuting Liang
author_sort Zhiyuan Ma
collection DOAJ
description Abstract Aluminium (Al)-tolerant beneficial bacteria confer resistance to Al toxicity to crops in widely distributed acidic soils. However, the mechanism by which microbial consortia maintain Al tolerance under acid and Al toxicity stress remains unknown. Here, we demonstrate that a soil bacterial consortium composed of Rhodococcus erythropolis and Pseudomonas aeruginosa exhibit greater Al tolerance than either bacterium alone. P. aeruginosa releases the quorum sensing molecule 2-heptyl-1H-quinolin-4-one (HHQ), which is efficiently degraded by R. erythropolis. This degradation reduces population density limitations and further enhances the metabolic activity of P. aeruginosa under Al stress. Moreover, R. erythropolis converts HHQ into tryptophan, promoting the synthesis of peptidoglycan, a key component for cell wall stability, thereby improving the Al tolerance of R. erythropolis. This study reveals a metabolic cross-feeding mechanism that maintains microbial Al tolerance, offering insights for designing synthetic microbial consortia to sustain food security and sustainable agriculture in acidic soil regions.
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spelling doaj-art-3d6152a0b3be4dbda2aabeec7500fa362025-08-20T02:33:02ZengNature PortfolioNature Communications2041-17232024-11-0115111210.1038/s41467-024-54616-0Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortiaZhiyuan Ma0Meitong Jiang1Chaoyang Liu2Ertao Wang3Yang Bai4Mengting Maggie Yuan5Shengjing Shi6Jizhong Zhou7Jixian Ding8Yimei Xie9Hui Zhang10Yan Yang11Renfang Shen12Thomas W. Crowther13Jiabao Zhang14Yuting Liang15State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, SIBS, Chinese Academy of SciencesSchool of Life Sciences, Peking UniversityDepartment of Environmental Science, Policy and Management, University of CaliforniaAgResearch Ltd, Lincoln Science CentreDepartment of Microbiology and Plant Biology, University of OklahomaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesDepartment of Environmental Systems Science, Institute of Integrative Biology, ETHState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesAbstract Aluminium (Al)-tolerant beneficial bacteria confer resistance to Al toxicity to crops in widely distributed acidic soils. However, the mechanism by which microbial consortia maintain Al tolerance under acid and Al toxicity stress remains unknown. Here, we demonstrate that a soil bacterial consortium composed of Rhodococcus erythropolis and Pseudomonas aeruginosa exhibit greater Al tolerance than either bacterium alone. P. aeruginosa releases the quorum sensing molecule 2-heptyl-1H-quinolin-4-one (HHQ), which is efficiently degraded by R. erythropolis. This degradation reduces population density limitations and further enhances the metabolic activity of P. aeruginosa under Al stress. Moreover, R. erythropolis converts HHQ into tryptophan, promoting the synthesis of peptidoglycan, a key component for cell wall stability, thereby improving the Al tolerance of R. erythropolis. This study reveals a metabolic cross-feeding mechanism that maintains microbial Al tolerance, offering insights for designing synthetic microbial consortia to sustain food security and sustainable agriculture in acidic soil regions.https://doi.org/10.1038/s41467-024-54616-0
spellingShingle Zhiyuan Ma
Meitong Jiang
Chaoyang Liu
Ertao Wang
Yang Bai
Mengting Maggie Yuan
Shengjing Shi
Jizhong Zhou
Jixian Ding
Yimei Xie
Hui Zhang
Yan Yang
Renfang Shen
Thomas W. Crowther
Jiabao Zhang
Yuting Liang
Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
Nature Communications
title Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
title_full Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
title_fullStr Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
title_full_unstemmed Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
title_short Quinolone-mediated metabolic cross-feeding develops aluminium tolerance in soil microbial consortia
title_sort quinolone mediated metabolic cross feeding develops aluminium tolerance in soil microbial consortia
url https://doi.org/10.1038/s41467-024-54616-0
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