Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity
This research aims to isolate, characterize, and analyze pure compounds from Leea macrophylla leaf extract to investigate its antibacterial, antidiabetic, cytotoxic, and phytotoxic effects. Fresh leaves were collected, dried, and subjected to methanol extraction to obtain a crude extract. From the p...
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Elsevier
2024-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240558082400205X |
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| author | Md. Selim Reza Md. Badrul Islam Samia Sharmin Farzana Mim A.B.M. Hamidul Haque Md. Sabir Hossain Farha Matin Juliana Subrata Banik Kazi Rasel Uddin Md. Mahmudul Hasan Salina Akter Afroza Parvin Md. Omar Ali Mondal |
| author_facet | Md. Selim Reza Md. Badrul Islam Samia Sharmin Farzana Mim A.B.M. Hamidul Haque Md. Sabir Hossain Farha Matin Juliana Subrata Banik Kazi Rasel Uddin Md. Mahmudul Hasan Salina Akter Afroza Parvin Md. Omar Ali Mondal |
| author_sort | Md. Selim Reza |
| collection | DOAJ |
| description | This research aims to isolate, characterize, and analyze pure compounds from Leea macrophylla leaf extract to investigate its antibacterial, antidiabetic, cytotoxic, and phytotoxic effects. Fresh leaves were collected, dried, and subjected to methanol extraction to obtain a crude extract. From the petroleum ether fraction (PEF) of this extract, three fractions—designated LM1, LM2, and LM3—were prepared using column chromatography. The fractions were tried to be characterized in search for single compound by instrumental technique like ATR-FTIR, 1H NMR and 13C NMR but the 1H NMR and 13C NMR spectra were found complex which were difficult to interpret. To dispel the doubt and get clear idea about the structure, GC-MS analysis of the compounds was carried out whose result showed that all the three extracts were decomposed to several small organic compounds that made the structure elucidation difficult. For this complication, the characterization of the extracts was not possible. Numerous compounds were identified in the methanol extract of L. macrophylla through GC-MS analysis. Among these compounds, Benzene, 1,2,3-trimethyl- and Undecane were found in higher percentages in LM1. LM2 contained Azulene and Bicyclo [4.4.1]undeca-1,3,5,7,9-pentaene, while LM3 was characterized by the presence of 9,9-Dimethoxybicyclo [3.3.1]nona-2,4-dione and 11-(2-Cyclopenten-1-yl)undecanoic acid, among others. The antibacterial activity of these fractions was evaluated against various bacterial strains, demonstrating broad-spectrum effectiveness. LM1 fraction showed the highest antibacterial activity against Proteus sp. With zone of inhibition 25 mm and weak activity against S. sonnei with zone of inhibition 5 mm. LM2 showed the highest activity to both E. cocci and P. aeruginosa with the zone of inhibition of 18 mm and comparatively lower but significant against Proteus sp. LM3 was highly active to S. sonnei with zone of inhibition 20 mm and lower but quite significant against Proteus sp. Moreover, the anti-diabetic potential was assessed, with LM1 showing the strongest α-amylase inhibitory activity, outperforming quercetin (standard). The IC50 values of LM1, LM2, LM3, and quercetin were 57.36 μg/mL, 100.66 μg/mL, 164.92 μg/mL, and 97.45 μg/mL, respectively. In addition, cytotoxicity was assessed using a brine shrimp lethality bioassay, and phytotoxicity was evaluated through seed germination and growth assays. The results suggest that L. macrophylla leaf extracts have potential applications in antimicrobial, antidiabetic, and anti-cancer contexts. This comprehensive study bridges gaps in knowledge surrounding L. macrophylla's multifaceted properties, offering insights into its therapeutic and ecological potential for healthcare and environmental management. |
| format | Article |
| id | doaj-art-3d5a89e8d8e94b488cf7dc38c11ec5f9 |
| institution | OA Journals |
| issn | 2405-5808 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-3d5a89e8d8e94b488cf7dc38c11ec5f92025-08-20T02:19:54ZengElsevierBiochemistry and Biophysics Reports2405-58082024-12-014010184110.1016/j.bbrep.2024.101841Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicityMd. Selim Reza0Md. Badrul Islam1Samia Sharmin2Farzana Mim3A.B.M. Hamidul Haque4Md. Sabir Hossain5Farha Matin Juliana6Subrata Banik7Kazi Rasel Uddin8Md. Mahmudul Hasan9Salina Akter10Afroza Parvin11Md. Omar Ali Mondal12Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, Bangladesh; Corresponding author. Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, Bangladesh.Natural Products Research Division, BCSIR Laboratories Rajshahi, Bangladesh Council of Scientific and Industrial Research, Rajshahi 6205, BangladeshDepartment of Chemistry, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Chemistry, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka 1342, BangladeshDurgapur Degree College, Rajshahi 6240, BangladeshThis research aims to isolate, characterize, and analyze pure compounds from Leea macrophylla leaf extract to investigate its antibacterial, antidiabetic, cytotoxic, and phytotoxic effects. Fresh leaves were collected, dried, and subjected to methanol extraction to obtain a crude extract. From the petroleum ether fraction (PEF) of this extract, three fractions—designated LM1, LM2, and LM3—were prepared using column chromatography. The fractions were tried to be characterized in search for single compound by instrumental technique like ATR-FTIR, 1H NMR and 13C NMR but the 1H NMR and 13C NMR spectra were found complex which were difficult to interpret. To dispel the doubt and get clear idea about the structure, GC-MS analysis of the compounds was carried out whose result showed that all the three extracts were decomposed to several small organic compounds that made the structure elucidation difficult. For this complication, the characterization of the extracts was not possible. Numerous compounds were identified in the methanol extract of L. macrophylla through GC-MS analysis. Among these compounds, Benzene, 1,2,3-trimethyl- and Undecane were found in higher percentages in LM1. LM2 contained Azulene and Bicyclo [4.4.1]undeca-1,3,5,7,9-pentaene, while LM3 was characterized by the presence of 9,9-Dimethoxybicyclo [3.3.1]nona-2,4-dione and 11-(2-Cyclopenten-1-yl)undecanoic acid, among others. The antibacterial activity of these fractions was evaluated against various bacterial strains, demonstrating broad-spectrum effectiveness. LM1 fraction showed the highest antibacterial activity against Proteus sp. With zone of inhibition 25 mm and weak activity against S. sonnei with zone of inhibition 5 mm. LM2 showed the highest activity to both E. cocci and P. aeruginosa with the zone of inhibition of 18 mm and comparatively lower but significant against Proteus sp. LM3 was highly active to S. sonnei with zone of inhibition 20 mm and lower but quite significant against Proteus sp. Moreover, the anti-diabetic potential was assessed, with LM1 showing the strongest α-amylase inhibitory activity, outperforming quercetin (standard). The IC50 values of LM1, LM2, LM3, and quercetin were 57.36 μg/mL, 100.66 μg/mL, 164.92 μg/mL, and 97.45 μg/mL, respectively. In addition, cytotoxicity was assessed using a brine shrimp lethality bioassay, and phytotoxicity was evaluated through seed germination and growth assays. The results suggest that L. macrophylla leaf extracts have potential applications in antimicrobial, antidiabetic, and anti-cancer contexts. This comprehensive study bridges gaps in knowledge surrounding L. macrophylla's multifaceted properties, offering insights into its therapeutic and ecological potential for healthcare and environmental management.http://www.sciencedirect.com/science/article/pii/S240558082400205XLeea macrophyllaLeaf extractAntibacterialAntidiabeticCytotoxicityPhytoxicity |
| spellingShingle | Md. Selim Reza Md. Badrul Islam Samia Sharmin Farzana Mim A.B.M. Hamidul Haque Md. Sabir Hossain Farha Matin Juliana Subrata Banik Kazi Rasel Uddin Md. Mahmudul Hasan Salina Akter Afroza Parvin Md. Omar Ali Mondal Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity Biochemistry and Biophysics Reports Leea macrophylla Leaf extract Antibacterial Antidiabetic Cytotoxicity Phytoxicity |
| title | Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity |
| title_full | Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity |
| title_fullStr | Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity |
| title_full_unstemmed | Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity |
| title_short | Isolation, characterization, and analysis of pure compounds from Leea macrophylla leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity |
| title_sort | isolation characterization and analysis of pure compounds from leea macrophylla leaf extract for antibacterial antidiabetic cytotoxicity and phytotoxicity |
| topic | Leea macrophylla Leaf extract Antibacterial Antidiabetic Cytotoxicity Phytoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S240558082400205X |
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