Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters
This study investigated the impact of light duration on Testosterone secretion in Chahua No.2 roosters, utilizing combined Transcriptome and Metabolome analyses to uncover critical genes, Metabolites, and signaling pathways. We randomly selected 240 Chahua No.2 roosters at 42 days old and divided th...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001555 |
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| author | Mengqian Liu Lei Tan Xinlu Li Haojie Li Yong Zhang Xiannian Zi Changrong Ge Kun Wang |
| author_facet | Mengqian Liu Lei Tan Xinlu Li Haojie Li Yong Zhang Xiannian Zi Changrong Ge Kun Wang |
| author_sort | Mengqian Liu |
| collection | DOAJ |
| description | This study investigated the impact of light duration on Testosterone secretion in Chahua No.2 roosters, utilizing combined Transcriptome and Metabolome analyses to uncover critical genes, Metabolites, and signaling pathways. We randomly selected 240 Chahua No.2 roosters at 42 days old and divided them into four groups: simulated natural light (Ⅰ), 12L:12D (Ⅱ), 16L:8D (Ⅲ), and 20L:4D (Ⅳ), each group has 6 replicates, with 10 chickens per replicate. Blood samples were collected at 91 and 140 days post-hatch to measure Testosterone levels. Results showed that at 91 days, group Ⅳ had significantly higher Testosterone levels than groups Ⅰ, Ⅱ, and Ⅲ (P < 0.01), with group Ⅲ also higher than groups Ⅰ (P < 0.01) and Ⅱ (P < 0.05). By 140 days, group Ⅳ maintained significantly higher Testosterone than groups Ⅰ and Ⅱ (P < 0.01) and higher than group Ⅲ (P < 0.05), while group Ⅲ was also elevated compared to groups Ⅰ and Ⅱ (P < 0.05). Testicles Transcriptomics analysis revealed 891 differentially expressed genes, including 479 down-regulated and 412 up-regulated genes. Key signaling pathways identified included Steroid Hormone Biosynthesis, Cytochrome P450, and retinol metabolism. Testicles Metabolomics analysis identified 174 differential Metabolites, with 91 up-regulated and 83 down-regulated, focusing on pathways like Amino Sugar Metabolism and Tryptophan Metabolism. Integrated analysis pinpointed 19 common signaling pathways, with the top ten including Cytochrome P450, tyrosine metabolism, and amino acid biosynthesis. Our findings indicate that extending light duration enhances Testosterone secretion in roosters. Through comprehensive transcriptomic and metabolomic analyses, we established that pathways associated with steroid hormone synthesis and Cytochrome P450 play a crucial role in light duration-regulated Testosterone secretion, highlighting key genes such as CYP11A1, CYP17A1, and HSD3B1, alongside Metabolites like ergosterol-5,7,22,24(28)-tetraene-3beta-alcohol. |
| format | Article |
| id | doaj-art-3d4ba75c37874a5384dcc53d1150bbf9 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-3d4ba75c37874a5384dcc53d1150bbf92025-08-20T02:18:04ZengElsevierPoultry Science0032-57912025-04-01104410491610.1016/j.psj.2025.104916Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 RoostersMengqian Liu0Lei Tan1Xinlu Li2Haojie Li3Yong Zhang4Xiannian Zi5Changrong Ge6Kun Wang7College of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, China; Key Laboratory of Feed Biotechnology, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Animal Science and Technology, Gansu Agricultural University, Gansu 730070, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Yunnan 650201, China; Corresponding author.This study investigated the impact of light duration on Testosterone secretion in Chahua No.2 roosters, utilizing combined Transcriptome and Metabolome analyses to uncover critical genes, Metabolites, and signaling pathways. We randomly selected 240 Chahua No.2 roosters at 42 days old and divided them into four groups: simulated natural light (Ⅰ), 12L:12D (Ⅱ), 16L:8D (Ⅲ), and 20L:4D (Ⅳ), each group has 6 replicates, with 10 chickens per replicate. Blood samples were collected at 91 and 140 days post-hatch to measure Testosterone levels. Results showed that at 91 days, group Ⅳ had significantly higher Testosterone levels than groups Ⅰ, Ⅱ, and Ⅲ (P < 0.01), with group Ⅲ also higher than groups Ⅰ (P < 0.01) and Ⅱ (P < 0.05). By 140 days, group Ⅳ maintained significantly higher Testosterone than groups Ⅰ and Ⅱ (P < 0.01) and higher than group Ⅲ (P < 0.05), while group Ⅲ was also elevated compared to groups Ⅰ and Ⅱ (P < 0.05). Testicles Transcriptomics analysis revealed 891 differentially expressed genes, including 479 down-regulated and 412 up-regulated genes. Key signaling pathways identified included Steroid Hormone Biosynthesis, Cytochrome P450, and retinol metabolism. Testicles Metabolomics analysis identified 174 differential Metabolites, with 91 up-regulated and 83 down-regulated, focusing on pathways like Amino Sugar Metabolism and Tryptophan Metabolism. Integrated analysis pinpointed 19 common signaling pathways, with the top ten including Cytochrome P450, tyrosine metabolism, and amino acid biosynthesis. Our findings indicate that extending light duration enhances Testosterone secretion in roosters. Through comprehensive transcriptomic and metabolomic analyses, we established that pathways associated with steroid hormone synthesis and Cytochrome P450 play a crucial role in light duration-regulated Testosterone secretion, highlighting key genes such as CYP11A1, CYP17A1, and HSD3B1, alongside Metabolites like ergosterol-5,7,22,24(28)-tetraene-3beta-alcohol.http://www.sciencedirect.com/science/article/pii/S0032579125001555TranscriptomeMetabolomelight intensityChahua No.2 roosterTestosterone |
| spellingShingle | Mengqian Liu Lei Tan Xinlu Li Haojie Li Yong Zhang Xiannian Zi Changrong Ge Kun Wang Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters Poultry Science Transcriptome Metabolome light intensity Chahua No.2 rooster Testosterone |
| title | Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters |
| title_full | Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters |
| title_fullStr | Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters |
| title_full_unstemmed | Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters |
| title_short | Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters |
| title_sort | integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in chahua chicken no 2 roosters |
| topic | Transcriptome Metabolome light intensity Chahua No.2 rooster Testosterone |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001555 |
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