Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks
The study aimed to estimate the levels of fructose, glucose, sucrose, and inositol levels in sweetened beverages with a newly developed method using HPLC coupled with a charged aerosol detector (CAD). In total, 85 commercially available non-alcoholic beverages, including 18 energy drinks, 8 sports d...
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MDPI AG
2024-10-01
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| Series: | Beverages |
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| Online Access: | https://www.mdpi.com/2306-5710/10/4/94 |
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| author | Małgorzata Grembecka Anna Lebiedzińska Piotr Szefer |
| author_facet | Małgorzata Grembecka Anna Lebiedzińska Piotr Szefer |
| author_sort | Małgorzata Grembecka |
| collection | DOAJ |
| description | The study aimed to estimate the levels of fructose, glucose, sucrose, and inositol levels in sweetened beverages with a newly developed method using HPLC coupled with a charged aerosol detector (CAD). In total, 85 commercially available non-alcoholic beverages, including 18 energy drinks, 8 sports drinks, 15 soft drinks, 14 fruit drinks, 7 fruit nectars, and 22 fruit juices were analyzed by HPLC-CAD. The method was validated, and it was characterized by a wide concentration range (1–150 µg/mL), sensitivity, and good accuracy (94.9–103%). The results showed significant variation in fructose, glucose, and sucrose concentrations in energy drinks, sports drinks, soft drinks, fruit drinks, fruit nectars, and juice. The highest total sugar contents (fructose, glucose, sucrose, and inositol) were found in energy drinks (14.2 g/100 mL), followed by fruit nectars (13.7 g/100 mL) and soft drinks (12.7 g/100 mL). Statistical analysis (Spearman correlation test, Kruskal–Wallis test) of the data showed significant relationships between particular sugars in the analyzed products. |
| format | Article |
| id | doaj-art-3d46e5f53e8b44c68f9a6c088db16122 |
| institution | Kabale University |
| issn | 2306-5710 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Beverages |
| spelling | doaj-art-3d46e5f53e8b44c68f9a6c088db161222024-12-27T14:11:14ZengMDPI AGBeverages2306-57102024-10-011049410.3390/beverages10040094Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft DrinksMałgorzata Grembecka0Anna Lebiedzińska1Piotr Szefer2Department of Bromatology, Medical University of Gdansk, Al. Gen. J. Hallera 107, 80-416 Gdansk, PolandThe Institute of Sport and Health Studies, State University of Applied Sciences in Koszalin, ul. Leśna 1, 75-582 Koszalin, PolandDepartment of Bromatology, Medical University of Gdansk, Al. Gen. J. Hallera 107, 80-416 Gdansk, PolandThe study aimed to estimate the levels of fructose, glucose, sucrose, and inositol levels in sweetened beverages with a newly developed method using HPLC coupled with a charged aerosol detector (CAD). In total, 85 commercially available non-alcoholic beverages, including 18 energy drinks, 8 sports drinks, 15 soft drinks, 14 fruit drinks, 7 fruit nectars, and 22 fruit juices were analyzed by HPLC-CAD. The method was validated, and it was characterized by a wide concentration range (1–150 µg/mL), sensitivity, and good accuracy (94.9–103%). The results showed significant variation in fructose, glucose, and sucrose concentrations in energy drinks, sports drinks, soft drinks, fruit drinks, fruit nectars, and juice. The highest total sugar contents (fructose, glucose, sucrose, and inositol) were found in energy drinks (14.2 g/100 mL), followed by fruit nectars (13.7 g/100 mL) and soft drinks (12.7 g/100 mL). Statistical analysis (Spearman correlation test, Kruskal–Wallis test) of the data showed significant relationships between particular sugars in the analyzed products.https://www.mdpi.com/2306-5710/10/4/94sweetened beveragessugarshigh-performance liquid chromatography |
| spellingShingle | Małgorzata Grembecka Anna Lebiedzińska Piotr Szefer Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks Beverages sweetened beverages sugars high-performance liquid chromatography |
| title | Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks |
| title_full | Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks |
| title_fullStr | Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks |
| title_full_unstemmed | Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks |
| title_short | Application of HPLC Coupled with a Charged Aerosol Detector to the Evaluation of Fructose, Glucose, Sucrose, and Inositol Levels in Fruit Juices, Energy Drinks, Sports Drinks, and Soft Drinks |
| title_sort | application of hplc coupled with a charged aerosol detector to the evaluation of fructose glucose sucrose and inositol levels in fruit juices energy drinks sports drinks and soft drinks |
| topic | sweetened beverages sugars high-performance liquid chromatography |
| url | https://www.mdpi.com/2306-5710/10/4/94 |
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