Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation
Abstract Five sustainable and validated UV spectrophotometric methods were developed for analyzing chloramphenicol (CHL) and dexamethasone sodium phosphate (DSP) in pure and ophthalmic dosage forms. CHL was detected by zero order spectra method at 292.0 nm in the range 2.00–32.00 µg/mL with limits o...
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Nature Portfolio
2025-04-01
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| author | Heidi R. Abd El-Hadi |
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| description | Abstract Five sustainable and validated UV spectrophotometric methods were developed for analyzing chloramphenicol (CHL) and dexamethasone sodium phosphate (DSP) in pure and ophthalmic dosage forms. CHL was detected by zero order spectra method at 292.0 nm in the range 2.00–32.00 µg/mL with limits of detection (LOD) and quantification (LOQ) of 0.96 and 2.88, respectively. DSP was analyzed using the following four techniques: Induce dual wavelength (IDW), fourier self-deconvolution (FSD), ratio difference (RD), and derivative ratio (DD1). The IDW method used at 239.0 and 254.0 nm with a linearity range of 4.00–40.00 µg/mL with LOD and LOQ values were 0.93 and 2.79, respectively. The FSD approach used at 242.0 nm, with a linearity range of 2.00–32.00 µg/mL and 0.65, 1.95 as values of LOD and LOQ, respectively. In the linearity range of 4.00–32.00 µg/mL, RD and DD1 are applied. RD is utilized at 225.0–240.0 nm, while DD1 is carried out at 249.0 nm. Values of LOD and LOQ for RD were 0.70 and 2.10 while for DD1 were 0.80 and 2.40, respectively. These methods were evaluated for their environmental sustainability and validated according to ICH guidelines, overcoming challenges like spectral overlap and collinearity. Statistical comparisons with published methods revealed no significant differences. |
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| spelling | doaj-art-3d418b82f8f345c0ae40328b92ce4b422025-08-20T02:19:58ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-96091-7Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimationHeidi R. Abd El-Hadi0Faculty of Pharmacy, Pharmaceutical Chemistry Department, Egyptian Russian UniversityAbstract Five sustainable and validated UV spectrophotometric methods were developed for analyzing chloramphenicol (CHL) and dexamethasone sodium phosphate (DSP) in pure and ophthalmic dosage forms. CHL was detected by zero order spectra method at 292.0 nm in the range 2.00–32.00 µg/mL with limits of detection (LOD) and quantification (LOQ) of 0.96 and 2.88, respectively. DSP was analyzed using the following four techniques: Induce dual wavelength (IDW), fourier self-deconvolution (FSD), ratio difference (RD), and derivative ratio (DD1). The IDW method used at 239.0 and 254.0 nm with a linearity range of 4.00–40.00 µg/mL with LOD and LOQ values were 0.93 and 2.79, respectively. The FSD approach used at 242.0 nm, with a linearity range of 2.00–32.00 µg/mL and 0.65, 1.95 as values of LOD and LOQ, respectively. In the linearity range of 4.00–32.00 µg/mL, RD and DD1 are applied. RD is utilized at 225.0–240.0 nm, while DD1 is carried out at 249.0 nm. Values of LOD and LOQ for RD were 0.70 and 2.10 while for DD1 were 0.80 and 2.40, respectively. These methods were evaluated for their environmental sustainability and validated according to ICH guidelines, overcoming challenges like spectral overlap and collinearity. Statistical comparisons with published methods revealed no significant differences.https://doi.org/10.1038/s41598-025-96091-7Spectrophotometric methodsChloramphenicolDexamethasone sodium phosphateGreennessWhitenessBlueness |
| spellingShingle | Heidi R. Abd El-Hadi Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation Scientific Reports Spectrophotometric methods Chloramphenicol Dexamethasone sodium phosphate Greenness Whiteness Blueness |
| title | Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| title_full | Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| title_fullStr | Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| title_full_unstemmed | Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| title_short | Comparative statistical evaluation of greenness, blueness, and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| title_sort | comparative statistical evaluation of greenness blueness and whiteness spectrophotometric methods for dexamethasone and chloramphenicol estimation |
| topic | Spectrophotometric methods Chloramphenicol Dexamethasone sodium phosphate Greenness Whiteness Blueness |
| url | https://doi.org/10.1038/s41598-025-96091-7 |
| work_keys_str_mv | AT heidirabdelhadi comparativestatisticalevaluationofgreennessbluenessandwhitenessspectrophotometricmethodsfordexamethasoneandchloramphenicolestimation |