Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients
Background: Breast cancer (BC) is considered the main cause of death in women worldwide. Overexpression of Sphingosine 1-phosphate (S1P) receptors in estrogen receptor (ER) negative BC patients is linked to poor prognosis. In vitro, the bioactive lipid metabolite S1P induced Nod-like receptor3 (NLRP...
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Ain Shams University
2023-12-01
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| Series: | Archives of Pharmaceutical Sciences Ain Shams University |
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| Online Access: | https://aps.journals.ekb.eg/article_329478.html |
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| author | Anas R. Soltan Amany M. Kamal Reham A. A. El-Shimy Hala O. El-Mesallamy |
| author_facet | Anas R. Soltan Amany M. Kamal Reham A. A. El-Shimy Hala O. El-Mesallamy |
| author_sort | Anas R. Soltan |
| collection | DOAJ |
| description | Background: Breast cancer (BC) is considered the main cause of death in women worldwide. Overexpression of Sphingosine 1-phosphate (S1P) receptors in estrogen receptor (ER) negative BC patients is linked to poor prognosis. In vitro, the bioactive lipid metabolite S1P induced Nod-like receptor3 (NLRP3) dependent activation of caspase-1 and secretion of interleukin-1beta (IL-1).
Objective: Evaluate serum levels of S1P and NLRP3 to examine their potential as diagnostic and prognostic biomarkers for BC.
Methods: The study involved 26 metastatic BC patients, 30 non-metastatic BC patients, and 20 healthy control volunteers. NLRP3, S1P, and cancer antigen15.3 (CA 15.3) serum levels were analyzed using ELISA. Statistical analysis involved Mann–Whitney U, Kruskall-Wallis tests, and Receiver operating curve (ROC) analysis.
Results: Serum levels of NLRP3 were significantly lower in non-metastatic than in metastatic BC (P < 0.001) and serum levels of S1P were significantly lower in metastatic than in non-metastatic BC and control (P < 0.001). Single ROC analysis showed that NLRP3 has a very good diagnostic value in early and late-stage BC (P< 0.0001), but a poor prognostic value in predicting metastasis (P=0.1392). In contrast, S1P has an excellent diagnostic value in late-stage BC (P <0.0001), and an excellent prognostic value in predicting metastasis (P<0.0001), but a poor diagnostic value in early-stage BC (P =0.1781). The diagnostic and prognostic value of the studied biomarkers improved by combining every two markers together (P<0.001).
Conclusion: NLRP3 and S1P could be promising novel diagnostic and prognostic biomarkers of BC either used alone or in combination with CA 15.3. |
| format | Article |
| id | doaj-art-3d342b7687854f9090ea1e77e365ce7a |
| institution | OA Journals |
| issn | 2356-8380 2356-8399 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Ain Shams University |
| record_format | Article |
| series | Archives of Pharmaceutical Sciences Ain Shams University |
| spelling | doaj-art-3d342b7687854f9090ea1e77e365ce7a2025-08-20T01:59:57ZengAin Shams UniversityArchives of Pharmaceutical Sciences Ain Shams University2356-83802356-83992023-12-0172334347https://doi.org/10.21608/aps.2023.226758.1130Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer PatientsAnas R. Soltan 0Amany M. Kamal1Reham A. A. El-Shimy 2Hala O. El-Mesallamy3 Fever Diseases Hospital, Damietta, Egypt.Department of Biochemistry, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.Department of Clinical and Chemical Pathology, National Cancer Institute NCI, Cairo University, Cairo, Egypt.Department of Biochemistry, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.Background: Breast cancer (BC) is considered the main cause of death in women worldwide. Overexpression of Sphingosine 1-phosphate (S1P) receptors in estrogen receptor (ER) negative BC patients is linked to poor prognosis. In vitro, the bioactive lipid metabolite S1P induced Nod-like receptor3 (NLRP3) dependent activation of caspase-1 and secretion of interleukin-1beta (IL-1). Objective: Evaluate serum levels of S1P and NLRP3 to examine their potential as diagnostic and prognostic biomarkers for BC. Methods: The study involved 26 metastatic BC patients, 30 non-metastatic BC patients, and 20 healthy control volunteers. NLRP3, S1P, and cancer antigen15.3 (CA 15.3) serum levels were analyzed using ELISA. Statistical analysis involved Mann–Whitney U, Kruskall-Wallis tests, and Receiver operating curve (ROC) analysis. Results: Serum levels of NLRP3 were significantly lower in non-metastatic than in metastatic BC (P < 0.001) and serum levels of S1P were significantly lower in metastatic than in non-metastatic BC and control (P < 0.001). Single ROC analysis showed that NLRP3 has a very good diagnostic value in early and late-stage BC (P< 0.0001), but a poor prognostic value in predicting metastasis (P=0.1392). In contrast, S1P has an excellent diagnostic value in late-stage BC (P <0.0001), and an excellent prognostic value in predicting metastasis (P<0.0001), but a poor diagnostic value in early-stage BC (P =0.1781). The diagnostic and prognostic value of the studied biomarkers improved by combining every two markers together (P<0.001). Conclusion: NLRP3 and S1P could be promising novel diagnostic and prognostic biomarkers of BC either used alone or in combination with CA 15.3.https://aps.journals.ekb.eg/article_329478.htmlnlrp3s1pdiagnosisbreast cancermetastasis. |
| spellingShingle | Anas R. Soltan Amany M. Kamal Reham A. A. El-Shimy Hala O. El-Mesallamy Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients Archives of Pharmaceutical Sciences Ain Shams University nlrp3 s1p diagnosis breast cancer metastasis. |
| title | Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients |
| title_full | Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients |
| title_fullStr | Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients |
| title_full_unstemmed | Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients |
| title_short | Serum NLRP3 and S1P as Potential Diagnostic and Prognostic Biomarkers in Female Metastatic and Non-Metastatic Breast Cancer Patients |
| title_sort | serum nlrp3 and s1p as potential diagnostic and prognostic biomarkers in female metastatic and non metastatic breast cancer patients |
| topic | nlrp3 s1p diagnosis breast cancer metastasis. |
| url | https://aps.journals.ekb.eg/article_329478.html |
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