Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis
Long non-coding RNA (lncRNA) H19 is an extensively studied lncRNA that is related to numerous pathological changes. Our previous findings have documented that serum lncRNA H19 levels are decreased in patients with chronic kidney disorder and lncRNA H19 reduction is closely correlated with renal tubu...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024000891 |
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| author | Yali Jiang Feng Ma Jing Wang Xiaojing Chen Lu Xue Xinping Chen Jinping Hu |
| author_facet | Yali Jiang Feng Ma Jing Wang Xiaojing Chen Lu Xue Xinping Chen Jinping Hu |
| author_sort | Yali Jiang |
| collection | DOAJ |
| description | Long non-coding RNA (lncRNA) H19 is an extensively studied lncRNA that is related to numerous pathological changes. Our previous findings have documented that serum lncRNA H19 levels are decreased in patients with chronic kidney disorder and lncRNA H19 reduction is closely correlated with renal tubulointerstitial fibrosis, an essential step in developing end-stage kidney disease. Nonetheless, the precise function and mechanism of lncRNA H19 in renal tubulointerstitial fibrosis are not fully comprehended. The present work utilized a mouse model of unilateral ureteral obstruction (UUO) and transforming growth factor-β1 (TGF-β1)-stimulated HK-2 cells to investigate the possible role and mechanism of lncRNA H19 in renal tubulointerstitial fibrosis were investigated. Levels of lncRNA H19 decreased in kidneys of mice with UUO and HK-2 cells stimulated with TGF-β1. Up-regulation of lncRNA H19 in mouse kidneys remarkably relieved kidney injury, fibrosis and inflammation triggered by UUO. Moreover, the increase of lncRNA H19 in HK-2 cells reduced epithelial-to-mesenchymal transition (EMT) induced by TGF-β1. Notably, up-regulation of lncRNA H19 reduced lipid accumulation and triacylglycerol content in kidneys of mice with UUO and TGF-β1-stimulated HK-2 cells, accompanied by the up-regulation of long-chain acyl-CoA synthetase 1 (ACSL1). lncRNA H19 was identified as a sponge of microRNA-130a-3p, through which lncRNA H19 modulates the expression of ACSL1. The overexpression of microRNA-130a-3p reversed the lncRNA H19-induced increases in the expression of ACSL1. The suppressive effects of lncRNA H19 overexpression on the EMT, inflammation and lipid accumulation in HK-2 cells were diminished by ACSL1 silencing or microRNA-130a-3p overexpression. Overall, the findings showed that lncRNA H19 ameliorated renal tubulointerstitial fibrosis by reducing lipid deposition via modulation of the microRNA-130a-3p/ACSL1 axis. |
| format | Article |
| id | doaj-art-3d2ffb4d666d4753b6c339883c684eaa |
| institution | OA Journals |
| issn | 2468-0540 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Non-coding RNA Research |
| spelling | doaj-art-3d2ffb4d666d4753b6c339883c684eaa2025-08-20T02:38:23ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-12-01941120113210.1016/j.ncrna.2024.05.002Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axisYali Jiang0Feng Ma1Jing Wang2Xiaojing Chen3Lu Xue4Xinping Chen5Jinping Hu6Department of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaDepartment of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaDepartment of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaDepartment of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaDepartment of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaDepartment of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaCorresponding author.; Department of Nephrology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi Province, ChinaLong non-coding RNA (lncRNA) H19 is an extensively studied lncRNA that is related to numerous pathological changes. Our previous findings have documented that serum lncRNA H19 levels are decreased in patients with chronic kidney disorder and lncRNA H19 reduction is closely correlated with renal tubulointerstitial fibrosis, an essential step in developing end-stage kidney disease. Nonetheless, the precise function and mechanism of lncRNA H19 in renal tubulointerstitial fibrosis are not fully comprehended. The present work utilized a mouse model of unilateral ureteral obstruction (UUO) and transforming growth factor-β1 (TGF-β1)-stimulated HK-2 cells to investigate the possible role and mechanism of lncRNA H19 in renal tubulointerstitial fibrosis were investigated. Levels of lncRNA H19 decreased in kidneys of mice with UUO and HK-2 cells stimulated with TGF-β1. Up-regulation of lncRNA H19 in mouse kidneys remarkably relieved kidney injury, fibrosis and inflammation triggered by UUO. Moreover, the increase of lncRNA H19 in HK-2 cells reduced epithelial-to-mesenchymal transition (EMT) induced by TGF-β1. Notably, up-regulation of lncRNA H19 reduced lipid accumulation and triacylglycerol content in kidneys of mice with UUO and TGF-β1-stimulated HK-2 cells, accompanied by the up-regulation of long-chain acyl-CoA synthetase 1 (ACSL1). lncRNA H19 was identified as a sponge of microRNA-130a-3p, through which lncRNA H19 modulates the expression of ACSL1. The overexpression of microRNA-130a-3p reversed the lncRNA H19-induced increases in the expression of ACSL1. The suppressive effects of lncRNA H19 overexpression on the EMT, inflammation and lipid accumulation in HK-2 cells were diminished by ACSL1 silencing or microRNA-130a-3p overexpression. Overall, the findings showed that lncRNA H19 ameliorated renal tubulointerstitial fibrosis by reducing lipid deposition via modulation of the microRNA-130a-3p/ACSL1 axis.http://www.sciencedirect.com/science/article/pii/S2468054024000891Long-chain acyl-CoA synthetase 1lncRNA H19Lipid metabolismmicroRNA-130a-3pRenal tubulointerstitial fibrosis |
| spellingShingle | Yali Jiang Feng Ma Jing Wang Xiaojing Chen Lu Xue Xinping Chen Jinping Hu Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis Non-coding RNA Research Long-chain acyl-CoA synthetase 1 lncRNA H19 Lipid metabolism microRNA-130a-3p Renal tubulointerstitial fibrosis |
| title | Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis |
| title_full | Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis |
| title_fullStr | Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis |
| title_full_unstemmed | Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis |
| title_short | Up-regulation of long non-coding RNA H19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microRNA-130a-3p/long-chain acyl-CoA synthetase 1 axis |
| title_sort | up regulation of long non coding rna h19 ameliorates renal tubulointerstitial fibrosis by reducing lipid deposition and inflammatory response through regulation of the microrna 130a 3p long chain acyl coa synthetase 1 axis |
| topic | Long-chain acyl-CoA synthetase 1 lncRNA H19 Lipid metabolism microRNA-130a-3p Renal tubulointerstitial fibrosis |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024000891 |
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