Mechanism of Electroacupuncture on Neuropathic Pain via Regulation of CD137L in Hippocampal Glia
ObjectiveTo investigate the mechanism of CD137L in CA1 region of hippocampus in improving mechanical pain and memory impairment in neuropathic pain rats during electroacupuncture therapy.MethodsA total of 24 SD rats were randomly divided into sham operation group, model group and electroacupuncture...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Office of Rehabilitation Medicine
2023-10-01
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| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2023.05005 |
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| Summary: | ObjectiveTo investigate the mechanism of CD137L in CA1 region of hippocampus in improving mechanical pain and memory impairment in neuropathic pain rats during electroacupuncture therapy.MethodsA total of 24 SD rats were randomly divided into sham operation group, model group and electroacupuncture group, with 8 rats in each group. In the model and electroacupuncture groups, the spared nerve injury (SNI) model was made. The sham operation group was just exposed right sciatic nerve without ligation. From the 7th day after modeling, the electroacupuncture group received electroacupuncture intervention in the right side Huantiao (GB30) and Yanglingquan (G34) acupoints, 30 minutes each time, once a day, lasting for 21 days. Rats in the model and the sham operation groups were equally grasped and fixed without any intervention. Von-Frey pain detector was used to measure the mechanical withdrawal threshold of rats. On the 28th day after modeling, new object recognition test was used to detect the learning and memory ability. The expression of hippocampal CA1 region CD137L, Iba-1, GFAP and tumor necrosis factor-α (TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA). Immunofluorescence was used for the cellular localization of CD137L and receptor CD137, and to measure the Iba-1 and GFAP expression in hippocampal CA1 region.Results1) Compared with the sham operation group, mechanical pain threshold and new object recognition index in the model group significantly decreased (<italic>P</italic><0.01); compared with the model group, mechanical pain threshold and new object recognition index in the electroacupuncture group significantly increased (<italic>P</italic><0.01). 2) Compared with the sham operation group, the concentrations of CD137L, Iba-1, GFAP and TNF-α in hippocampus of rats in the model group significantly increased (<italic>P</italic><0.01), and the number of positive Iba-1 and GFAP immune reactions significantly increased (<italic>P</italic><0.01); compared with the model group, the concentrations of CD137L, Iba-1, GFAP and TNF-α in hippocampus of rats in the electroacupuncture group significantly decreased (all <italic>P</italic><0.05), and the number of positive immune reactions of Iba-1 and GFAP significantly decreased (all <italic>P</italic><0.01). 3) Immunofluorescence detection showed that CD137L and its receptor CD137 were significantly co-expressed with microglia marker (Iba-1) and astrocyte marker (GFAP) in hippocampal CA1 region, suggesting that CD137L and CD137 were mainly expressed in microglia and astrocytes, but not in neurons.ConclusionElectroacupuncture may play an analgesic role by reducing the expression of glial CD137L, the amount of activated glial cells and the concentrations of TNF-α. |
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| ISSN: | 2096-0328 |