Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and interstitial disease with an unclear cause, believed to involve genetic, environmental, and molecular factors. Recent research suggested that <i>Pneumocystis jirovecii</i> (PJ) could contribute to disease exacerbations an...

Full description

Saved in:
Bibliographic Details
Main Authors: Jonás Carmona-Pírez, Rocío Salsoso, Eléna Charpentier, Cinta Olmedo, Francisco J. Medrano, Lucas Román, Carmen de la Horra, Yaxsier de Armas, Enrique J. Calderón, Vicente Friaza
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Journal of Fungi
Subjects:
Online Access:https://www.mdpi.com/2309-608X/11/2/102
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849719881649356800
author Jonás Carmona-Pírez
Rocío Salsoso
Eléna Charpentier
Cinta Olmedo
Francisco J. Medrano
Lucas Román
Carmen de la Horra
Yaxsier de Armas
Enrique J. Calderón
Vicente Friaza
author_facet Jonás Carmona-Pírez
Rocío Salsoso
Eléna Charpentier
Cinta Olmedo
Francisco J. Medrano
Lucas Román
Carmen de la Horra
Yaxsier de Armas
Enrique J. Calderón
Vicente Friaza
author_sort Jonás Carmona-Pírez
collection DOAJ
description Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and interstitial disease with an unclear cause, believed to involve genetic, environmental, and molecular factors. Recent research suggested that <i>Pneumocystis jirovecii</i> (PJ) could contribute to disease exacerbations and severity. This article explores how PJ colonization might influence the pathogenesis of IPF. We performed a proteomic analysis to study the profile of control and IPF patients, with/without PJ. We recruited nine participants from the Virgen del Rocio University Hospital (Seville, Spain). iTRAQ and bioinformatics analyses were performed to identify differentially expressed proteins (DEPs), including a functional analysis of DEPs and of the protein–protein interaction networks built using the STRING database. We identified a total of 92 DEPs highlighting the protein vimentin when comparing groups. Functional differences were observed, with the glycolysis pathway highlighted in PJ-colonized IPF patients; as well as the pentose phosphate pathway and miR-133A in non-colonized IPF patients. We found 11 protein complexes, notably the JAK-STAT signaling complex in non-colonized IPF patients. To our knowledge, this is the first study that analyzed PJ colonization’s effect on IPF patients. However, further research is needed, especially on the complex interactions with the AKT/GSK-3β/snail pathway that could explain some of our results.
format Article
id doaj-art-3d2e8a9d0f5f41e6817d22f5edb1ce56
institution DOAJ
issn 2309-608X
language English
publishDate 2025-01-01
publisher MDPI AG
record_format Article
series Journal of Fungi
spelling doaj-art-3d2e8a9d0f5f41e6817d22f5edb1ce562025-08-20T03:12:02ZengMDPI AGJournal of Fungi2309-608X2025-01-0111210210.3390/jof11020102Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary FibrosisJonás Carmona-Pírez0Rocío Salsoso1Eléna Charpentier2Cinta Olmedo3Francisco J. Medrano4Lucas Román5Carmen de la Horra6Yaxsier de Armas7Enrique J. Calderón8Vicente Friaza9Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainUnidad Médico-Quirúrgica de Enfermedades Respiratorias, Hospital Universitario Virgen del Rocío, 41013 Seville, SpainInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainUnidad Médico-Quirúrgica de Enfermedades Respiratorias, Hospital Universitario Virgen del Rocío, 41013 Seville, SpainInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainDepartments of Clinical Microbiology Diagnostic and Pathology, Hospital Center of Institute of Tropical Medicine “Pedro Kourí”, Havana 11400, CubaInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainInstituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013 Seville, SpainIdiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and interstitial disease with an unclear cause, believed to involve genetic, environmental, and molecular factors. Recent research suggested that <i>Pneumocystis jirovecii</i> (PJ) could contribute to disease exacerbations and severity. This article explores how PJ colonization might influence the pathogenesis of IPF. We performed a proteomic analysis to study the profile of control and IPF patients, with/without PJ. We recruited nine participants from the Virgen del Rocio University Hospital (Seville, Spain). iTRAQ and bioinformatics analyses were performed to identify differentially expressed proteins (DEPs), including a functional analysis of DEPs and of the protein–protein interaction networks built using the STRING database. We identified a total of 92 DEPs highlighting the protein vimentin when comparing groups. Functional differences were observed, with the glycolysis pathway highlighted in PJ-colonized IPF patients; as well as the pentose phosphate pathway and miR-133A in non-colonized IPF patients. We found 11 protein complexes, notably the JAK-STAT signaling complex in non-colonized IPF patients. To our knowledge, this is the first study that analyzed PJ colonization’s effect on IPF patients. However, further research is needed, especially on the complex interactions with the AKT/GSK-3β/snail pathway that could explain some of our results.https://www.mdpi.com/2309-608X/11/2/102idiopathic pulmonary fibrosisproteomics<i>Pneumocystis jirovecii</i><i>Pneumocystis</i> colonizationiTRAQ quantificationprotein–protein interaction networks
spellingShingle Jonás Carmona-Pírez
Rocío Salsoso
Eléna Charpentier
Cinta Olmedo
Francisco J. Medrano
Lucas Román
Carmen de la Horra
Yaxsier de Armas
Enrique J. Calderón
Vicente Friaza
Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
Journal of Fungi
idiopathic pulmonary fibrosis
proteomics
<i>Pneumocystis jirovecii</i>
<i>Pneumocystis</i> colonization
iTRAQ quantification
protein–protein interaction networks
title Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
title_full Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
title_fullStr Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
title_full_unstemmed Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
title_short Proteomic Approach to Study the Effect of <i>Pneumocystis jirovecii</i> Colonization in Idiopathic Pulmonary Fibrosis
title_sort proteomic approach to study the effect of i pneumocystis jirovecii i colonization in idiopathic pulmonary fibrosis
topic idiopathic pulmonary fibrosis
proteomics
<i>Pneumocystis jirovecii</i>
<i>Pneumocystis</i> colonization
iTRAQ quantification
protein–protein interaction networks
url https://www.mdpi.com/2309-608X/11/2/102
work_keys_str_mv AT jonascarmonapirez proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT rociosalsoso proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT elenacharpentier proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT cintaolmedo proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT franciscojmedrano proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT lucasroman proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT carmendelahorra proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT yaxsierdearmas proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT enriquejcalderon proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis
AT vicentefriaza proteomicapproachtostudytheeffectofipneumocystisjiroveciiicolonizationinidiopathicpulmonaryfibrosis