A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum
Peptidoglycan recognition protein (PGRP) is a kind of conserved pattern recognition receptors (PRRs) and plays important roles in innate immune response in both invertebrates and vertebrates. In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cD...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311625000084 |
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| author | Yinan Li Hongbo Lu Chuanyan Yang Zhuyun Liu Weilin Wang Chang Liu Lingling Wang |
| author_facet | Yinan Li Hongbo Lu Chuanyan Yang Zhuyun Liu Weilin Wang Chang Liu Lingling Wang |
| author_sort | Yinan Li |
| collection | DOAJ |
| description | Peptidoglycan recognition protein (PGRP) is a kind of conserved pattern recognition receptors (PRRs) and plays important roles in innate immune response in both invertebrates and vertebrates. In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cDNA of MgPGRP5 which was consisted of a SH3b and a PGRP domain was cloned. The mRNA transcripts of MgPGRP5 were constitutively expressed in haemocytes and all the examined tissues, with the highest expression level in hepatopancreas. Both PGN and LPS stimulation could significantly up-regulated the expression level of MgPGRP5 mRNA in haemocytes (p < 0.05). Two amino acids in MgPGRP5, Asp230 and Glu106, were identified as potential binding sites for PGN and LPS. The MgPGRP5 protein was mainly located in nucleus of haemocytes, and showed widespread expression in hepatopancreas. The recombinant MgPGRP5 protein (rMgPGRP5) displayed strong binding and agglutinated activities to Gram-negative bacteria, Gram-positive bacteria and fungi. These results indicated that MgPGRP5 could function as PRR with a broad range of recognition spectrum in the innate immune response of oyster. |
| format | Article |
| id | doaj-art-3d275a4dcf794b2d8cd5683b06e5d97a |
| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Comparative Immunology Reports |
| spelling | doaj-art-3d275a4dcf794b2d8cd5683b06e5d97a2025-01-29T05:02:56ZengElsevierComparative Immunology Reports2950-31162025-06-018200202A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrumYinan Li0Hongbo Lu1Chuanyan Yang2Zhuyun Liu3Weilin Wang4Chang Liu5Lingling Wang6Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Ocean, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Ocean, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Ocean, Dalian, China; Corresponding author at: Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, China.Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Ocean, Dalian, ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Ocean, Dalian, ChinaPeptidoglycan recognition protein (PGRP) is a kind of conserved pattern recognition receptors (PRRs) and plays important roles in innate immune response in both invertebrates and vertebrates. In the present study, nine MgPGRP genes were identified in the updated genome of Magallana gigas, and the cDNA of MgPGRP5 which was consisted of a SH3b and a PGRP domain was cloned. The mRNA transcripts of MgPGRP5 were constitutively expressed in haemocytes and all the examined tissues, with the highest expression level in hepatopancreas. Both PGN and LPS stimulation could significantly up-regulated the expression level of MgPGRP5 mRNA in haemocytes (p < 0.05). Two amino acids in MgPGRP5, Asp230 and Glu106, were identified as potential binding sites for PGN and LPS. The MgPGRP5 protein was mainly located in nucleus of haemocytes, and showed widespread expression in hepatopancreas. The recombinant MgPGRP5 protein (rMgPGRP5) displayed strong binding and agglutinated activities to Gram-negative bacteria, Gram-positive bacteria and fungi. These results indicated that MgPGRP5 could function as PRR with a broad range of recognition spectrum in the innate immune response of oyster.http://www.sciencedirect.com/science/article/pii/S2950311625000084SH3b domain-containing PGRPMagallana gigasInnate immunityPattern recognition receptor |
| spellingShingle | Yinan Li Hongbo Lu Chuanyan Yang Zhuyun Liu Weilin Wang Chang Liu Lingling Wang A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum Comparative Immunology Reports SH3b domain-containing PGRP Magallana gigas Innate immunity Pattern recognition receptor |
| title | A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum |
| title_full | A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum |
| title_fullStr | A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum |
| title_full_unstemmed | A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum |
| title_short | A SH3b domain-containing peptidoglycan recognition protein from Magallana gigas with broad recognition spectrum |
| title_sort | sh3b domain containing peptidoglycan recognition protein from magallana gigas with broad recognition spectrum |
| topic | SH3b domain-containing PGRP Magallana gigas Innate immunity Pattern recognition receptor |
| url | http://www.sciencedirect.com/science/article/pii/S2950311625000084 |
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