High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea
Anthracnose crown rot caused by Colletotrichum spp. is one of the most important diseases of strawberry (Fragaria × ananassa) in Korea. Single spore isolates of 72 Colletotrichum species collected in 2018, 2020, and 2023 from different locations in Chungbuk, Chungnam, Gyeongbuk, Gyeongnam, and Jeoll...
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Hanrimwon Publishing Company
2024-12-01
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| Series: | Research in Plant Disease |
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| Online Access: | http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-372.pdf |
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| author | Abdullahi Abdulkareem Myeong Hyeon Nam Subin Park Young Ju Nam Heung Tae Kim |
| author_facet | Abdullahi Abdulkareem Myeong Hyeon Nam Subin Park Young Ju Nam Heung Tae Kim |
| author_sort | Abdullahi Abdulkareem |
| collection | DOAJ |
| description | Anthracnose crown rot caused by Colletotrichum spp. is one of the most important diseases of strawberry (Fragaria × ananassa) in Korea. Single spore isolates of 72 Colletotrichum species collected in 2018, 2020, and 2023 from different locations in Chungbuk, Chungnam, Gyeongbuk, Gyeongnam, and Jeolla regions were characterized using a multi-locus sequence typing (MLST) of four genetic loci (GAPDH, ACT, TUB2, and ITS). Isolates were identified as C. fructicola (n=64) and C. siamense (n=8). Quinone outside inhibitors (QoIs) active ingredient pyraclostrobin is utilized to manage anthracnose crown rot in the field. Sensitivity to pyraclostrobin were assessed on potato dextrose agar using a mycelial growth inhibition assay at 20, 4, 0.8, 0.16, and 0.032 µg/ml fungicide concentrations. Our results demonstrated that all the 72 Colletotrichum spp. isolates were not inhibited by pyraclostrobin at all the tested fungicide concentrations, with EC50 ranging between 13 and >100 µg/ml. Sequences of the partial cytochrome b (cyt b) gene flanking the mutation site revealed the presence of the G143A mutation in all the isolates. No other site modifications were detected in the cyt b gene. Cross resistance assays with 10 selected isolates involving EC50 values of pyraclostrobin, mandestrobin, trifloxystrobin, and kresoxim-methyl showed positive correlations among these fungicides. These findings suggest a high risk of resistance development associated with using QoI fungicides to control the anthracnose crown rot of strawberries in Korea. |
| format | Article |
| id | doaj-art-3d163eff0c4f41e386645d06dfd48bf5 |
| institution | DOAJ |
| issn | 1598-2262 2233-9191 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Hanrimwon Publishing Company |
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| series | Research in Plant Disease |
| spelling | doaj-art-3d163eff0c4f41e386645d06dfd48bf52025-08-20T03:04:38ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912024-12-0130437238010.5423/RPD.2024.30.4.3721870High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in KoreaAbdullahi Abdulkareem0Myeong Hyeon Nam1Subin Park2Young Ju Nam3Heung Tae Kim4Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University, Cheongju 28644, KoreaStrawberry Research Institute, Chungnam ARES, Nonsan 32914, KoreaGlobal Agro-Consulting Corporation, Suwon 16614, KoreaGlobal Agro-Consulting Corporation, Suwon 16614, KoreaDepartment of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University, Cheongju 28644, KoreaAnthracnose crown rot caused by Colletotrichum spp. is one of the most important diseases of strawberry (Fragaria × ananassa) in Korea. Single spore isolates of 72 Colletotrichum species collected in 2018, 2020, and 2023 from different locations in Chungbuk, Chungnam, Gyeongbuk, Gyeongnam, and Jeolla regions were characterized using a multi-locus sequence typing (MLST) of four genetic loci (GAPDH, ACT, TUB2, and ITS). Isolates were identified as C. fructicola (n=64) and C. siamense (n=8). Quinone outside inhibitors (QoIs) active ingredient pyraclostrobin is utilized to manage anthracnose crown rot in the field. Sensitivity to pyraclostrobin were assessed on potato dextrose agar using a mycelial growth inhibition assay at 20, 4, 0.8, 0.16, and 0.032 µg/ml fungicide concentrations. Our results demonstrated that all the 72 Colletotrichum spp. isolates were not inhibited by pyraclostrobin at all the tested fungicide concentrations, with EC50 ranging between 13 and >100 µg/ml. Sequences of the partial cytochrome b (cyt b) gene flanking the mutation site revealed the presence of the G143A mutation in all the isolates. No other site modifications were detected in the cyt b gene. Cross resistance assays with 10 selected isolates involving EC50 values of pyraclostrobin, mandestrobin, trifloxystrobin, and kresoxim-methyl showed positive correlations among these fungicides. These findings suggest a high risk of resistance development associated with using QoI fungicides to control the anthracnose crown rot of strawberries in Korea.http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-372.pdfcolletotrichum spp.cyt b geneqoi fungicidesstrawberry anthracnose |
| spellingShingle | Abdullahi Abdulkareem Myeong Hyeon Nam Subin Park Young Ju Nam Heung Tae Kim High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea Research in Plant Disease colletotrichum spp. cyt b gene qoi fungicides strawberry anthracnose |
| title | High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea |
| title_full | High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea |
| title_fullStr | High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea |
| title_full_unstemmed | High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea |
| title_short | High Quinone Outside Inhibitor (QoI) Resistance Risk in Colletotrichum Species Causing Strawberry Crown Rot in Korea |
| title_sort | high quinone outside inhibitor qoi resistance risk in colletotrichum species causing strawberry crown rot in korea |
| topic | colletotrichum spp. cyt b gene qoi fungicides strawberry anthracnose |
| url | http://www.online-rpd.org/upload/pdf/RPD-2024-30-4-372.pdf |
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