Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile.
Filamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional identification methods are time-consuming, labor-intensive, and operator-dependent. Matrix-assisted laser desorption/...
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0315690 |
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| author | Lorena Porte Rodrigo Cruz Inia Pérez Carmen Varela Cristina Díaz Patricia García Paulette Legarraga Francisca Valdivieso Thomas Weitzel |
| author_facet | Lorena Porte Rodrigo Cruz Inia Pérez Carmen Varela Cristina Díaz Patricia García Paulette Legarraga Francisca Valdivieso Thomas Weitzel |
| author_sort | Lorena Porte |
| collection | DOAJ |
| description | Filamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional identification methods are time-consuming, labor-intensive, and operator-dependent. Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry is a rapid and easy-to-perform identification method. The effectiveness of a commercial MALDI-TOF MS platform to identify filamentous fungi in a clinical laboratory was evaluated. The study included 67 fungal isolates from 35 species/species complexes, which were identified and confirmed in mycology reference laboratories; 32 derived from clinical samples, 34 from strain collections and one was an ATCC strain. The study used the VITEK® MS system (v3.2.0 database), after sample extraction by VITEK® MS Mould Kit. Results were classified as "correct species", "correct species complex", "correct genus"and "no identification". VITEK® MS correctly identified 91.0% of isolates (58.2% to species, 29.9% to species complex, and 1.5% to genus level only). In 82%, the result matched the species/species complex identified by reference methods. No misidentifications were observed. The kit was rapid and easy to use. In conclusion, the VITEK® MS system showed a high capability to accurately identify filamentous fungi in a clinical laboratory. |
| format | Article |
| id | doaj-art-3cf2ef662776465894a288575fd1df63 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-3cf2ef662776465894a288575fd1df632025-08-20T02:35:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031569010.1371/journal.pone.0315690Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile.Lorena PorteRodrigo CruzInia PérezCarmen VarelaCristina DíazPatricia GarcíaPaulette LegarragaFrancisca ValdiviesoThomas WeitzelFilamentous fungi are an emergent cause of severe infections in immunocompromised patients. Timely and accurate identification is crucial to initiate appropriate therapy. Traditional identification methods are time-consuming, labor-intensive, and operator-dependent. Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry is a rapid and easy-to-perform identification method. The effectiveness of a commercial MALDI-TOF MS platform to identify filamentous fungi in a clinical laboratory was evaluated. The study included 67 fungal isolates from 35 species/species complexes, which were identified and confirmed in mycology reference laboratories; 32 derived from clinical samples, 34 from strain collections and one was an ATCC strain. The study used the VITEK® MS system (v3.2.0 database), after sample extraction by VITEK® MS Mould Kit. Results were classified as "correct species", "correct species complex", "correct genus"and "no identification". VITEK® MS correctly identified 91.0% of isolates (58.2% to species, 29.9% to species complex, and 1.5% to genus level only). In 82%, the result matched the species/species complex identified by reference methods. No misidentifications were observed. The kit was rapid and easy to use. In conclusion, the VITEK® MS system showed a high capability to accurately identify filamentous fungi in a clinical laboratory.https://doi.org/10.1371/journal.pone.0315690 |
| spellingShingle | Lorena Porte Rodrigo Cruz Inia Pérez Carmen Varela Cristina Díaz Patricia García Paulette Legarraga Francisca Valdivieso Thomas Weitzel Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. PLoS ONE |
| title | Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. |
| title_full | Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. |
| title_fullStr | Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. |
| title_full_unstemmed | Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. |
| title_short | Performance of the VITEK® MS system for the identification of filamentous fungi in a microbiological laboratory in Chile. |
| title_sort | performance of the vitek r ms system for the identification of filamentous fungi in a microbiological laboratory in chile |
| url | https://doi.org/10.1371/journal.pone.0315690 |
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