Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard
Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also...
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International Viticulture and Enology Society
2024-11-01
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| Series: | OENO One |
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| Online Access: | https://oeno-one.eu/article/view/8280 |
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| author | Daniela Mezzatesta Mónica Oyuela Aguilar Alex Gobbi Mariano Pistorio Lars Hestbjerg Hansen Fernando Buscema Patricia Piccoli Frederico Berli |
| author_facet | Daniela Mezzatesta Mónica Oyuela Aguilar Alex Gobbi Mariano Pistorio Lars Hestbjerg Hansen Fernando Buscema Patricia Piccoli Frederico Berli |
| author_sort | Daniela Mezzatesta |
| collection | DOAJ |
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Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza's mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions.
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| format | Article |
| id | doaj-art-3cece77efc5e4c8f8530e4b1068e3985 |
| institution | OA Journals |
| issn | 2494-1271 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | International Viticulture and Enology Society |
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| series | OENO One |
| spelling | doaj-art-3cece77efc5e4c8f8530e4b1068e39852025-08-20T02:33:06ZengInternational Viticulture and Enology SocietyOENO One2494-12712024-11-0158410.20870/oeno-one.2024.58.4.8280Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyardDaniela Mezzatesta0Mónica Oyuela Aguilar1Alex Gobbi2Mariano Pistorio3Lars Hestbjerg Hansen4Fernando Buscema5Patricia Piccoli6Frederico Berli7Instituto de Biología Agrícola de Mendoza (IBAM), CONICET-Universidad Nacional de Cuyo, Facultad de Ciencias Agrarias. Almirante Brown 500 (5507) Chacras de Coria, Mendoza, Argentina. / Catena Institute of Wine, Cobos s/n (5509), Agrelo, Mendoza, Argentina.Instituto de Biotecnología y Biología Molecular (IBBM), CONICET-Universidad Nacional de La Plata, Facultad de Ciencias Exactas, Calles 49 y 115 (1900) La Plata, Argentina.CREA – Council for Agricultural Research and Economics, Research Centre for Agriculture and Environment, I-40128 Bologna, Italy. / Department of Plant and Environmental Science (PLEN), University of Copenhagen, Frederiksberg 1821, Copenhagen, Denmark.Instituto de Biotecnología y Biología Molecular (IBBM), CONICET-Universidad Nacional de La Plata, Facultad de Ciencias Exactas, Calles 49 y 115 (1900) La Plata, Argentina.Department of Plant and Environmental Science (PLEN), University of Copenhagen, Frederiksberg 1821, Copenhagen, Denmark.Catena Institute of Wine, Cobos s/n (5509), Agrelo, Mendoza, Argentina.Instituto de Biología Agrícola de Mendoza (IBAM), CONICET-Universidad Nacional de Cuyo, Facultad de Ciencias Agrarias. Almirante Brown 500 (5507) Chacras de Coria, Mendoza, Argentina.Instituto de Biología Agrícola de Mendoza (IBAM), CONICET-Universidad Nacional de Cuyo, Facultad de Ciencias Agrarias. Almirante Brown 500 (5507) Chacras de Coria, Mendoza, Argentina. Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza's mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions. https://oeno-one.eu/article/view/8280metabarcodinggrapevinesoil microbiometerroirArgentinaMendoza |
| spellingShingle | Daniela Mezzatesta Mónica Oyuela Aguilar Alex Gobbi Mariano Pistorio Lars Hestbjerg Hansen Fernando Buscema Patricia Piccoli Frederico Berli Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard OENO One metabarcoding grapevine soil microbiome terroir Argentina Mendoza |
| title | Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
| title_full | Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
| title_fullStr | Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
| title_full_unstemmed | Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
| title_short | Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
| title_sort | soil associated fungal and prokaryotic diversity influenced by stoniness depth and vintage in a high altitude vineyard |
| topic | metabarcoding grapevine soil microbiome terroir Argentina Mendoza |
| url | https://oeno-one.eu/article/view/8280 |
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