Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA
Background: Insomnia, the most prevalent sleep disorder, is clinically defined as difficulty initiating or maintaining sleep. Although many medications are effective for insomnia treatment, they carry risks of drug dependence and abuse. The microbiota-gut-brain axis (MGBA) facilit...
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| Format: | Article |
| Language: | English |
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IMR Press
2025-06-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL39587 |
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| author | Yongcheng Jiang Lina Guo Houde He Haonan Chen Tao Chen Yan Liu Wang Zhao |
| author_facet | Yongcheng Jiang Lina Guo Houde He Haonan Chen Tao Chen Yan Liu Wang Zhao |
| author_sort | Yongcheng Jiang |
| collection | DOAJ |
| description | Background: Insomnia, the most prevalent sleep disorder, is clinically defined as difficulty initiating or maintaining sleep. Although many medications are effective for insomnia treatment, they carry risks of drug dependence and abuse. The microbiota-gut-brain axis (MGBA) facilitates bidirectional signaling between the gastrointestinal tract and the central nervous system via gut microbes. Probiotics that provide mental and behavioral benefits through MGBA (psychobiotics) offer broad therapeutic potential. Methods: A non-toxic, drug-resistant strain of Lactobacillus reuteri E9 was isolated and characterized. Its effects were evaluated in a pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder. Neurotransmitter levels (glycine, serine, taurine, γ-aminobutyric acid (GABA)) and gene expression of GABA/melatonin receptors were analyzed. Results: E9 significantly upregulated inhibitory neurotransmitters, including GABA, taurine, glycine, and serine (p < 0.05). In PTZ-induced zebrafish, E9 exerted sedative effects by reducing seizures and hyperactivity. Concurrently, E9 upregulated the expression of GABA receptor genes and melatonin receptor (Mtnr1aa) genes in zebrafish neural tissue. Conclusions: Lactobacillus reuteri E9 demonstrates potential as a psychobiotic for sleep disorder management by modulating key inhibitory neurotransmitters and sleep-related receptor expression via the MGBA pathway, offering a non-pharmacological alternative to conventional treatments. |
| format | Article |
| id | doaj-art-3ce6ddfaff8540c1b265456425fbacda |
| institution | Kabale University |
| issn | 2768-6701 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj-art-3ce6ddfaff8540c1b265456425fbacda2025-08-20T03:33:08ZengIMR PressFrontiers in Bioscience-Landmark2768-67012025-06-013063958710.31083/FBL39587S2768-6701(25)01763-0Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABAYongcheng Jiang0Lina Guo1Houde He2Haonan Chen3Tao Chen4Yan Liu5Wang Zhao6Neurology Department, The People's Hospital of Chizhou, 247100 Chizhou, Anhui, ChinaClinical Nutrition Department, Guangdong Provincial Hospital of Chinese Medicine, 510030 Guangzhou, Guangdong, ChinaSchool of Public Health, Southern Medical University, 528305 Guangzhou, Guangdong, ChinaThe First Clinical Medical School, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, ChinaResearch and Development Center, Center of Human Microecology Engineering and Technology of Guangdong Province, 510700 Guangzhou, Guangdong, ChinaResearch and Development Center, Center of Human Microecology Engineering and Technology of Guangdong Province, 510700 Guangzhou, Guangdong, ChinaNeurology Department, The People's Hospital of Chizhou, 247100 Chizhou, Anhui, ChinaBackground: Insomnia, the most prevalent sleep disorder, is clinically defined as difficulty initiating or maintaining sleep. Although many medications are effective for insomnia treatment, they carry risks of drug dependence and abuse. The microbiota-gut-brain axis (MGBA) facilitates bidirectional signaling between the gastrointestinal tract and the central nervous system via gut microbes. Probiotics that provide mental and behavioral benefits through MGBA (psychobiotics) offer broad therapeutic potential. Methods: A non-toxic, drug-resistant strain of Lactobacillus reuteri E9 was isolated and characterized. Its effects were evaluated in a pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder. Neurotransmitter levels (glycine, serine, taurine, γ-aminobutyric acid (GABA)) and gene expression of GABA/melatonin receptors were analyzed. Results: E9 significantly upregulated inhibitory neurotransmitters, including GABA, taurine, glycine, and serine (p < 0.05). In PTZ-induced zebrafish, E9 exerted sedative effects by reducing seizures and hyperactivity. Concurrently, E9 upregulated the expression of GABA receptor genes and melatonin receptor (Mtnr1aa) genes in zebrafish neural tissue. Conclusions: Lactobacillus reuteri E9 demonstrates potential as a psychobiotic for sleep disorder management by modulating key inhibitory neurotransmitters and sleep-related receptor expression via the MGBA pathway, offering a non-pharmacological alternative to conventional treatments.https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL39587lactobacillus reuterigamma-aminobutyric acidsleep disorderszebrafish |
| spellingShingle | Yongcheng Jiang Lina Guo Houde He Haonan Chen Tao Chen Yan Liu Wang Zhao Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA Frontiers in Bioscience-Landmark lactobacillus reuteri gamma-aminobutyric acid sleep disorders zebrafish |
| title | Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA |
| title_full | Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA |
| title_fullStr | Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA |
| title_full_unstemmed | Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA |
| title_short | Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA |
| title_sort | lactobacillus reuteri e9 regulates sleep disorders through its metabolite gaba |
| topic | lactobacillus reuteri gamma-aminobutyric acid sleep disorders zebrafish |
| url | https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL39587 |
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