Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)

This study aims to determine the effect of monosodium glutamate on LH and testosterone levels in serum of adult male rats (Rattus norvegicus). A total of 12 male Wistar-strained rats divided into three groups with 4 rats per group. Control group was given distilled water, while M1 was given MSG at a...

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Main Authors: Riska Annisa, Moch. Sasmito Djati, Sri Rahayu
Format: Article
Language:English
Published: University of Brawijaya 2019-06-01
Series:Journal of Experimental Life Science
Subjects:
Online Access:https://jels.ub.ac.id/index.php/jels/article/view/341
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author Riska Annisa
Moch. Sasmito Djati
Sri Rahayu
author_facet Riska Annisa
Moch. Sasmito Djati
Sri Rahayu
author_sort Riska Annisa
collection DOAJ
description This study aims to determine the effect of monosodium glutamate on LH and testosterone levels in serum of adult male rats (Rattus norvegicus). A total of 12 male Wistar-strained rats divided into three groups with 4 rats per group. Control group was given distilled water, while M1 was given MSG at a dose of 4mg g-1 BW for 15 days and M2 was given MSG at a dose of 4 mg g-1BW for 45 days. All substances were given orally. At the end of the study, rats were sacrificed with a dislocation to draw blood from the heart. Then, the blood was incubated at room temperature to obtain serum. Serum was used to analyze LH and testosterone levels using the ELISA kit. Data were analyzed using one way ANOVA (p ≤ 0.05) with SPPS 16.0 for windows. The results showed that serum LH levels after MSG treatments for 45 days was significantly decreased compared with the control. The serum testosterone levels M2 was significantly difference with the control group (p ≤ 0.01). This study predicts that monosodium glutamate have a direct and indirect effect on male reproductive organs.
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record_format Article
series Journal of Experimental Life Science
spelling doaj-art-3ce3afe1e169479a904b0aae9052e8ff2025-08-20T03:49:41ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552019-06-0192105109https://doi.org/10.21776/ub.jels.2019.009.02.07Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)Riska Annisa0Moch. Sasmito Djati1Sri Rahayu2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaThis study aims to determine the effect of monosodium glutamate on LH and testosterone levels in serum of adult male rats (Rattus norvegicus). A total of 12 male Wistar-strained rats divided into three groups with 4 rats per group. Control group was given distilled water, while M1 was given MSG at a dose of 4mg g-1 BW for 15 days and M2 was given MSG at a dose of 4 mg g-1BW for 45 days. All substances were given orally. At the end of the study, rats were sacrificed with a dislocation to draw blood from the heart. Then, the blood was incubated at room temperature to obtain serum. Serum was used to analyze LH and testosterone levels using the ELISA kit. Data were analyzed using one way ANOVA (p ≤ 0.05) with SPPS 16.0 for windows. The results showed that serum LH levels after MSG treatments for 45 days was significantly decreased compared with the control. The serum testosterone levels M2 was significantly difference with the control group (p ≤ 0.01). This study predicts that monosodium glutamate have a direct and indirect effect on male reproductive organs.https://jels.ub.ac.id/index.php/jels/article/view/341luteinizing hormonemonosodium glutamatetestosterone
spellingShingle Riska Annisa
Moch. Sasmito Djati
Sri Rahayu
Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
Journal of Experimental Life Science
luteinizing hormone
monosodium glutamate
testosterone
title Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
title_full Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
title_fullStr Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
title_full_unstemmed Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
title_short Effects of Monosodium Glutamate Oral Administration on LH and Testosterone Levels in Serum of Adult Male Rats (Rattus norvegicus)
title_sort effects of monosodium glutamate oral administration on lh and testosterone levels in serum of adult male rats rattus norvegicus
topic luteinizing hormone
monosodium glutamate
testosterone
url https://jels.ub.ac.id/index.php/jels/article/view/341
work_keys_str_mv AT riskaannisa effectsofmonosodiumglutamateoraladministrationonlhandtestosteronelevelsinserumofadultmaleratsrattusnorvegicus
AT mochsasmitodjati effectsofmonosodiumglutamateoraladministrationonlhandtestosteronelevelsinserumofadultmaleratsrattusnorvegicus
AT srirahayu effectsofmonosodiumglutamateoraladministrationonlhandtestosteronelevelsinserumofadultmaleratsrattusnorvegicus