Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA
This study aimed to reveal intraspecific variations in two <i>Dryomys</i> species distributed in Türkiye, based on mitochondrial DNA cytochrome <i>b</i> gene sequences, and to discuss the factors driving these variations in the context of phylogeography and genetic species co...
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| author | Ercüment Çolak Georgi Markov Engin Selvi Teoman Kankılıç Perinçek Seçkinozan Şeker Maria A. Kocheva Milena K. Gospodinova Reyhan Çolak Hristo Dimitrov Nuri Yiğit |
| author_facet | Ercüment Çolak Georgi Markov Engin Selvi Teoman Kankılıç Perinçek Seçkinozan Şeker Maria A. Kocheva Milena K. Gospodinova Reyhan Çolak Hristo Dimitrov Nuri Yiğit |
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| description | This study aimed to reveal intraspecific variations in two <i>Dryomys</i> species distributed in Türkiye, based on mitochondrial DNA cytochrome <i>b</i> gene sequences, and to discuss the factors driving these variations in the context of phylogeography and genetic species concepts. As a result of Maximum Likelihood, Bayesian Inference, and Network analyses, which included haplogroups or lineages from Italy, Russia, the Caucasus, and Iran identified in previous studies, along with Turkish haplotypes, three major clades (MC1, MC2, and MC3) were identified within <i>Dryomys nitedula</i>. These clades began to diverge evolutionarily in the middle of the Late Miocene (8.82 million years ago) and exhibit significant genetic differences from one another. The Turkish haplotypes were divided into five distinct lineages (N1–N5), each within five subclades (SC1–SC5), which were nested within these MCs. These lineages, their geographical distributions, and the subspecies defined in previous studies that correspond to these lineages are as follows: N1 from the Thrace region (<i>Dryomys nitedula wingei</i>), N2 from the Black Sea region (potentially a new subspecies), N3 from western and central Anatolia (<i>Dryomys nitedula phrygius</i>), N4 from northeastern Anatolia (<i>Dryomys nitedula tichomirowi</i>), and N5 from eastern Anatolia (<i>Dryomys nitedula pictus</i>). The N2 lineage, distributed in areas close to the coastal side of the Eastern Black Sea region and with a range close to both N3 (<i>D. n. phrygius</i>) and N4 (<i>D. n. tichomirowi</i>), exhibited high genetic differentiation from these two lineages and was a candidate to be treated as a new subspecies of <i>Dryomys nitedula</i> in Türkiye. The N5 lineage, which includes haplotypes from the distribution areas of the populations initially classified as <i>Dryomys pictus</i> and later as <i>Dryomys nitedula pictus</i> in previous studies, was found to be more closely related to <i>Dryomys nitedula kurdistanicus</i> from the Zagros Mountains than to <i>D. n. pictus</i> from the central regions of Iran. Combining the results of this study with previous research, it is clear that the <i>D. nitedula</i> lineages in Türkiye, along with haplogroups or subspecies in neighboring regions diverged between the middle Late Miocene and Middle Pleistocene. This divergence is believed to have been driven by climatic cycles and geomorphological processes that shaped the topography of their distribution range. The high genetic diversity observed in the lineages of Anatolia suggests that the region may have served as a glacial refuge for <i>D. nitedula</i>. Similarly to the processes and factors shaping the evolution of <i>D. nitedula</i>, <i>Dryomys laniger</i> was found to have diverged into two lineages, western (L1) and eastern (L2 or <i>Dryomys anatolicus</i>), within its distribution range during the Late Pliocene (2.94 Mya). To make a more accurate taxonomic assessment of <i>D. laniger</i>, a larger number of samples is needed, and the distribution limits should be more clearly defined. |
| format | Article |
| id | doaj-art-3cd84a4f32f344e9a4a6338195cfa64a |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-3cd84a4f32f344e9a4a6338195cfa64a2025-08-20T03:13:52ZengMDPI AGLife2075-17292025-04-0115466010.3390/life15040660Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNAErcüment Çolak0Georgi Markov1Engin Selvi2Teoman Kankılıç3Perinçek Seçkinozan Şeker4Maria A. Kocheva5Milena K. Gospodinova6Reyhan Çolak7Hristo Dimitrov8Nuri Yiğit9Department of Biology, Faculty of Science, Ankara University, Ankara 06100, TürkiyeInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Biology, Faculty of Science, Ankara University, Ankara 06100, TürkiyeDepartment of Biotechnology, Faculty of Science and Letters, Ömer Halisdemir University, Niğde 51000, TürkiyeDepartment of Forestry, Artvin Vocational School, Artvin Çoruh University, Artvin 08100, TürkiyeInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Biology, Faculty of Science, Ankara University, Ankara 06100, TürkiyeDepartment of Zoology, Faculty of Biology, Plovdiv University, 4000 Plovdiv, BulgariaDepartment of Biology, Faculty of Science, Ankara University, Ankara 06100, TürkiyeThis study aimed to reveal intraspecific variations in two <i>Dryomys</i> species distributed in Türkiye, based on mitochondrial DNA cytochrome <i>b</i> gene sequences, and to discuss the factors driving these variations in the context of phylogeography and genetic species concepts. As a result of Maximum Likelihood, Bayesian Inference, and Network analyses, which included haplogroups or lineages from Italy, Russia, the Caucasus, and Iran identified in previous studies, along with Turkish haplotypes, three major clades (MC1, MC2, and MC3) were identified within <i>Dryomys nitedula</i>. These clades began to diverge evolutionarily in the middle of the Late Miocene (8.82 million years ago) and exhibit significant genetic differences from one another. The Turkish haplotypes were divided into five distinct lineages (N1–N5), each within five subclades (SC1–SC5), which were nested within these MCs. These lineages, their geographical distributions, and the subspecies defined in previous studies that correspond to these lineages are as follows: N1 from the Thrace region (<i>Dryomys nitedula wingei</i>), N2 from the Black Sea region (potentially a new subspecies), N3 from western and central Anatolia (<i>Dryomys nitedula phrygius</i>), N4 from northeastern Anatolia (<i>Dryomys nitedula tichomirowi</i>), and N5 from eastern Anatolia (<i>Dryomys nitedula pictus</i>). The N2 lineage, distributed in areas close to the coastal side of the Eastern Black Sea region and with a range close to both N3 (<i>D. n. phrygius</i>) and N4 (<i>D. n. tichomirowi</i>), exhibited high genetic differentiation from these two lineages and was a candidate to be treated as a new subspecies of <i>Dryomys nitedula</i> in Türkiye. The N5 lineage, which includes haplotypes from the distribution areas of the populations initially classified as <i>Dryomys pictus</i> and later as <i>Dryomys nitedula pictus</i> in previous studies, was found to be more closely related to <i>Dryomys nitedula kurdistanicus</i> from the Zagros Mountains than to <i>D. n. pictus</i> from the central regions of Iran. Combining the results of this study with previous research, it is clear that the <i>D. nitedula</i> lineages in Türkiye, along with haplogroups or subspecies in neighboring regions diverged between the middle Late Miocene and Middle Pleistocene. This divergence is believed to have been driven by climatic cycles and geomorphological processes that shaped the topography of their distribution range. The high genetic diversity observed in the lineages of Anatolia suggests that the region may have served as a glacial refuge for <i>D. nitedula</i>. Similarly to the processes and factors shaping the evolution of <i>D. nitedula</i>, <i>Dryomys laniger</i> was found to have diverged into two lineages, western (L1) and eastern (L2 or <i>Dryomys anatolicus</i>), within its distribution range during the Late Pliocene (2.94 Mya). To make a more accurate taxonomic assessment of <i>D. laniger</i>, a larger number of samples is needed, and the distribution limits should be more clearly defined.https://www.mdpi.com/2075-1729/15/4/660<i>Dryomys nitedula</i><i>Dryomys laniger</i>intraspecific variationcytochrome <i>b</i>phylogeographyphylogeny |
| spellingShingle | Ercüment Çolak Georgi Markov Engin Selvi Teoman Kankılıç Perinçek Seçkinozan Şeker Maria A. Kocheva Milena K. Gospodinova Reyhan Çolak Hristo Dimitrov Nuri Yiğit Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA Life <i>Dryomys nitedula</i> <i>Dryomys laniger</i> intraspecific variation cytochrome <i>b</i> phylogeography phylogeny |
| title | Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA |
| title_full | Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA |
| title_fullStr | Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA |
| title_full_unstemmed | Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA |
| title_short | Assessment of Intraspecific Variability in the Forest Dormouse (<i>Dryomys nitedula</i>) and Woolly Dormouse (<i>Dryomys laniger</i>) from Türkiye and Adjacent Regions Based on Mitochondrial DNA |
| title_sort | assessment of intraspecific variability in the forest dormouse i dryomys nitedula i and woolly dormouse i dryomys laniger i from turkiye and adjacent regions based on mitochondrial dna |
| topic | <i>Dryomys nitedula</i> <i>Dryomys laniger</i> intraspecific variation cytochrome <i>b</i> phylogeography phylogeny |
| url | https://www.mdpi.com/2075-1729/15/4/660 |
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