Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics
This experiment aimed to investigate the differences in metabolites of different varieties from Xinjiang large-fruit sea buckthorn, ultra high performance liquid chromatography-mass spectrometry (UPLC-QTOF-MS) coupled with multivariate statistical analysis and pathway enrichment analysis were used t...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050420 |
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| author | Rong HAN Yan MA Shengjie LI Xintao MENG Mingqiang XU Ting ZHANG Yan PAN |
| author_facet | Rong HAN Yan MA Shengjie LI Xintao MENG Mingqiang XU Ting ZHANG Yan PAN |
| author_sort | Rong HAN |
| collection | DOAJ |
| description | This experiment aimed to investigate the differences in metabolites of different varieties from Xinjiang large-fruit sea buckthorn, ultra high performance liquid chromatography-mass spectrometry (UPLC-QTOF-MS) coupled with multivariate statistical analysis and pathway enrichment analysis were used to characterize the metabolites of six Xinjiang large-fruit sea buckthorn species ('Mixed Fruit', 'Giant', 'Shenqunhon', 'Wucifeng', 'Xiangyang' and 'Zhuangyuan Yellow') were subjected to non-targeted metabolomics study. The results showed that a total of 334 metabolites were obtained from the metabolomic analysis. Based on log2FC absolute value≥1 and FDR<0.05 analysis, a total of 112 differential metabolites were screened between 'Mixed Fruit' and 'Giant'. 'Mixed Fruit' and 'Shenqunhon' screened a total of 118 differential metabolites. 'Mixed Fruit' and 'Wucifeng' screened a total of 67 differential metabolites. A total of 160 differential metabolites were screened between 'Mixed Fruit' and 'Xiangyang' and a total of 126 differential metabolites were screened between 'Mixed Fruit' and 'Zhuangyuan Yellow'. The metabolic pathway enrichment analysis showed that the differential metabolites of different large-fruit sea buckthorns were mainly enriched in the pathways of unsaturated fatty acid biosynthesis, fatty acid biosynthesis, tryptophan metabolism, and flavonoid and flavonol biosynthesis. Based on the VIP value>1, log2FC absolute value≥1 and FDR<0.05 screening criteria, a total of five differential metabolites were found, and overall high abundance and lipid-like molecules, phenylpropanes and polyketides were found in sea buckthorn, ranging from 38% to 81% and 15% to 49%, respectively, among which erucic acid amide, linoleic acid, palmitic acid, narcissin, isorhamnetin-3-O-glucoside, rutin, etc. might be the key metabolites to identify different Xinjiang large-fruit sea buckthorn. Non-targeted metabolomics can elucidate the metabolic differences of different varieties of Xinjiang large-fruit sea buckthorn at the overall level, which provides scientific basis for the selection and breeding of Xinjiang large-fruit sea buckthorn varieties and their processing and utilization. |
| format | Article |
| id | doaj-art-3cd2fa490f09402a88cd34dbfbeda57e |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-3cd2fa490f09402a88cd34dbfbeda57e2025-02-08T08:06:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-01464192910.13386/j.issn1002-0306.20240504202024050420-4Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted MetabolomicsRong HAN0Yan MA1Shengjie LI2Xintao MENG3Mingqiang XU4Ting ZHANG5Yan PAN6College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaInstitute of Storage and Processing of Agricultural Products, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaInstitute of Storage and Processing of Agricultural Products, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, ChinaInstitute of Storage and Processing of Agricultural Products, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, ChinaInstitute of Storage and Processing of Agricultural Products, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, ChinaInstitute of Storage and Processing of Agricultural Products, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, ChinaThis experiment aimed to investigate the differences in metabolites of different varieties from Xinjiang large-fruit sea buckthorn, ultra high performance liquid chromatography-mass spectrometry (UPLC-QTOF-MS) coupled with multivariate statistical analysis and pathway enrichment analysis were used to characterize the metabolites of six Xinjiang large-fruit sea buckthorn species ('Mixed Fruit', 'Giant', 'Shenqunhon', 'Wucifeng', 'Xiangyang' and 'Zhuangyuan Yellow') were subjected to non-targeted metabolomics study. The results showed that a total of 334 metabolites were obtained from the metabolomic analysis. Based on log2FC absolute value≥1 and FDR<0.05 analysis, a total of 112 differential metabolites were screened between 'Mixed Fruit' and 'Giant'. 'Mixed Fruit' and 'Shenqunhon' screened a total of 118 differential metabolites. 'Mixed Fruit' and 'Wucifeng' screened a total of 67 differential metabolites. A total of 160 differential metabolites were screened between 'Mixed Fruit' and 'Xiangyang' and a total of 126 differential metabolites were screened between 'Mixed Fruit' and 'Zhuangyuan Yellow'. The metabolic pathway enrichment analysis showed that the differential metabolites of different large-fruit sea buckthorns were mainly enriched in the pathways of unsaturated fatty acid biosynthesis, fatty acid biosynthesis, tryptophan metabolism, and flavonoid and flavonol biosynthesis. Based on the VIP value>1, log2FC absolute value≥1 and FDR<0.05 screening criteria, a total of five differential metabolites were found, and overall high abundance and lipid-like molecules, phenylpropanes and polyketides were found in sea buckthorn, ranging from 38% to 81% and 15% to 49%, respectively, among which erucic acid amide, linoleic acid, palmitic acid, narcissin, isorhamnetin-3-O-glucoside, rutin, etc. might be the key metabolites to identify different Xinjiang large-fruit sea buckthorn. Non-targeted metabolomics can elucidate the metabolic differences of different varieties of Xinjiang large-fruit sea buckthorn at the overall level, which provides scientific basis for the selection and breeding of Xinjiang large-fruit sea buckthorn varieties and their processing and utilization.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050420xinjiang large-fruit sea buckthornnon-targeted metabolomicsdifferential metabolitesuplc-qtof-ms |
| spellingShingle | Rong HAN Yan MA Shengjie LI Xintao MENG Mingqiang XU Ting ZHANG Yan PAN Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics Shipin gongye ke-ji xinjiang large-fruit sea buckthorn non-targeted metabolomics differential metabolites uplc-qtof-ms |
| title | Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics |
| title_full | Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics |
| title_fullStr | Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics |
| title_full_unstemmed | Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics |
| title_short | Differences in Metabolites of Different Varieties from Xinjiang Large-fruit Sea Buckthorn as Analyzed by Non-targeted Metabolomics |
| title_sort | differences in metabolites of different varieties from xinjiang large fruit sea buckthorn as analyzed by non targeted metabolomics |
| topic | xinjiang large-fruit sea buckthorn non-targeted metabolomics differential metabolites uplc-qtof-ms |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050420 |
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