Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma
Objectives: Resveratrol and oxyresveratrol, a resveratrol derivative, possess various pharmacological activities, including anti-cancer activities. Because cancer stem cells play an important role in cancer recurrence, the aims of this study were to investigate whether resveratrol or oxyresveratrol...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426822000148 |
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| author | Artid Amorntaveechai Thanaphum Osathanon Prasit Pavasant Sireerat Sooampon |
| author_facet | Artid Amorntaveechai Thanaphum Osathanon Prasit Pavasant Sireerat Sooampon |
| author_sort | Artid Amorntaveechai |
| collection | DOAJ |
| description | Objectives: Resveratrol and oxyresveratrol, a resveratrol derivative, possess various pharmacological activities, including anti-cancer activities. Because cancer stem cells play an important role in cancer recurrence, the aims of this study were to investigate whether resveratrol or oxyresveratrol can inhibit the expression of cancer stem cell markers under hypoxia. Materials and methods: Deferoxamine was used to mimic the hypoxic condition. The mRNA expression of cancer stem cell markers was analyzed by Real-time PCR. Flow cytometry was used to determine the number of CD-44+ and CD-105+ cells. Results: Deferoxamine dose-dependently induced the expression of cancer stem cell markers; Oct-4, Nanog, CD-44, CD-105, and CD-133. The induction of these cancer stem cells markers was inhibited when the cells were treated with either resveratrol or oxyresveratrol. Moreover, we found that resveratrol also reduced the number of CD-44+ and CD-105+ cells after deferoxamine treatment. Conclusions: Resveratrol and oxyresveratrol inhibit the expression of cancer stem cell markers and might target cancer stem cells in a hypoxia-associated tumor. |
| format | Article |
| id | doaj-art-3cc5d4c929e14524b8c9e054d74edb56 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-3cc5d4c929e14524b8c9e054d74edb562024-11-23T06:29:11ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682022-03-01122253257Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinomaArtid Amorntaveechai0Thanaphum Osathanon1Prasit Pavasant2Sireerat Sooampon3Interdepartmental Program of Pharmacology, Graduate School, Chulalongkorn University, ThailandDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn University, ThailandDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn University, ThailandDepartment of Pharmacology, Faculty of Dentistry, Chulalongkorn University, Thailand; Corresponding author. Department of Pharmacology, Faculty of Dentistry, Chulalongkorn University, 34 Henri-Dunant Road, Pathumwan, Bangkok, 10330, Thailand.Objectives: Resveratrol and oxyresveratrol, a resveratrol derivative, possess various pharmacological activities, including anti-cancer activities. Because cancer stem cells play an important role in cancer recurrence, the aims of this study were to investigate whether resveratrol or oxyresveratrol can inhibit the expression of cancer stem cell markers under hypoxia. Materials and methods: Deferoxamine was used to mimic the hypoxic condition. The mRNA expression of cancer stem cell markers was analyzed by Real-time PCR. Flow cytometry was used to determine the number of CD-44+ and CD-105+ cells. Results: Deferoxamine dose-dependently induced the expression of cancer stem cell markers; Oct-4, Nanog, CD-44, CD-105, and CD-133. The induction of these cancer stem cells markers was inhibited when the cells were treated with either resveratrol or oxyresveratrol. Moreover, we found that resveratrol also reduced the number of CD-44+ and CD-105+ cells after deferoxamine treatment. Conclusions: Resveratrol and oxyresveratrol inhibit the expression of cancer stem cell markers and might target cancer stem cells in a hypoxia-associated tumor.http://www.sciencedirect.com/science/article/pii/S2212426822000148ResveratrolOxyresveratrolHead and neck cancer cell carcinomaCancer stem cellsDeferoxamine |
| spellingShingle | Artid Amorntaveechai Thanaphum Osathanon Prasit Pavasant Sireerat Sooampon Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma Journal of Oral Biology and Craniofacial Research Resveratrol Oxyresveratrol Head and neck cancer cell carcinoma Cancer stem cells Deferoxamine |
| title | Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| title_full | Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| title_fullStr | Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| title_full_unstemmed | Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| title_short | Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| title_sort | effect of resveratrol and oxyresveratrol on deferoxamine induced cancer stem cell marker expression in human head and neck squamous cell carcinoma |
| topic | Resveratrol Oxyresveratrol Head and neck cancer cell carcinoma Cancer stem cells Deferoxamine |
| url | http://www.sciencedirect.com/science/article/pii/S2212426822000148 |
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