Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles

Microsatellites have been identified as the marker of choice in plant genotyping projects. However, due to length discrepancies obtained between different laboratories for the same allele, interlaboratory comparison of fingerprinting results is often a difficult task. The objectives of this study we...

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Main Authors: Matjaž HLADNIK, Jernej JAKŠE, Bouchaib KHADARI, Sylvain SANTONI, Dunja BANDELJ
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 2018-04-01
Series:Acta Agriculturae Slovenica
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Online Access:https://journals.uni-lj.si/aas/article/view/12761
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author Matjaž HLADNIK
Jernej JAKŠE
Bouchaib KHADARI
Sylvain SANTONI
Dunja BANDELJ
author_facet Matjaž HLADNIK
Jernej JAKŠE
Bouchaib KHADARI
Sylvain SANTONI
Dunja BANDELJ
author_sort Matjaž HLADNIK
collection DOAJ
description Microsatellites have been identified as the marker of choice in plant genotyping projects. However, due to length discrepancies obtained between different laboratories for the same allele, interlaboratory comparison of fingerprinting results is often a difficult task. The objectives of this study were to compare genotyping results of two laboratories, to evaluate genetic parameters of microsatellite markers and to determine reference allele sizes for fig cultivars from the Istrian peninsula. Genotyping results of ninety fig (Ficus carica L.) accessions were comparable between the laboratories despite differences observed when comparing electropherograms of different capillary electrophoresis systems. Differences in lengths of the same alleles were detected due to different PCR methods and laboratory equipment, but the distances between alleles of the same locus were preserved. However, locus FSYC01 exhibited one allele dropout which led to misidentification of 28 heterozygotes as homozygote individuals suggesting this locus as unreliable. Allele dropout was assigned to the tail PCR technology or to a touchdown PCR protocol. Genotypes of twenty-four reference cultivars from the Istrian peninsula were confirmed by both laboratories. These results will contribute to the usage of markers with greater reliability, discrimination power and consequently, to more reliable standardization with other fig genotyping projects.
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spelling doaj-art-3cbeb5a53aa045e68902afe046557bfc2025-08-20T02:58:55ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412018-04-01111114315910.14720/aas.2018.111.1.1419153Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference allelesMatjaž HLADNIK0Jernej JAKŠE1Bouchaib KHADARI2Sylvain SANTONI3Dunja BANDELJ4University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Glagoljaška 8, SI-6000 Koper, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaNational Institute of Agricultural Research (INRA), UMR Genetic Improvement and Adaptation of Mediterranean and Tropical Plants AGAP 1334, F-34070 Montpellier, FranceNational Institute of Agricultural Research (INRA), UMR Genetic Improvement and Adaptation of Mediterranean and Tropical Plants AGAP 1334, F-34070 Montpellier, FranceUniversity of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Glagoljaška 8, SI-6000 Koper, SloveniaMicrosatellites have been identified as the marker of choice in plant genotyping projects. However, due to length discrepancies obtained between different laboratories for the same allele, interlaboratory comparison of fingerprinting results is often a difficult task. The objectives of this study were to compare genotyping results of two laboratories, to evaluate genetic parameters of microsatellite markers and to determine reference allele sizes for fig cultivars from the Istrian peninsula. Genotyping results of ninety fig (Ficus carica L.) accessions were comparable between the laboratories despite differences observed when comparing electropherograms of different capillary electrophoresis systems. Differences in lengths of the same alleles were detected due to different PCR methods and laboratory equipment, but the distances between alleles of the same locus were preserved. However, locus FSYC01 exhibited one allele dropout which led to misidentification of 28 heterozygotes as homozygote individuals suggesting this locus as unreliable. Allele dropout was assigned to the tail PCR technology or to a touchdown PCR protocol. Genotypes of twenty-four reference cultivars from the Istrian peninsula were confirmed by both laboratories. These results will contribute to the usage of markers with greater reliability, discrimination power and consequently, to more reliable standardization with other fig genotyping projects.https://journals.uni-lj.si/aas/article/view/12761microsatellite markerreference genotypeinterlaboratory comparisonficus carica l.
spellingShingle Matjaž HLADNIK
Jernej JAKŠE
Bouchaib KHADARI
Sylvain SANTONI
Dunja BANDELJ
Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
Acta Agriculturae Slovenica
microsatellite marker
reference genotype
interlaboratory comparison
ficus carica l.
title Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
title_full Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
title_fullStr Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
title_full_unstemmed Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
title_short Interlaboratory comparison of fig (Ficus carica L.) microsatellite genotyping data and determination of reference alleles
title_sort interlaboratory comparison of fig ficus carica l microsatellite genotyping data and determination of reference alleles
topic microsatellite marker
reference genotype
interlaboratory comparison
ficus carica l.
url https://journals.uni-lj.si/aas/article/view/12761
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