Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs
IntroductionCurrent point-of-care testing for canine-specific pancreatic lipase (CPL) provides semi-quantitative measurements with binary results. Recently, a commercial point-of-care testing method (Vcheck CPL) that offers quantitative measurement of CPL has emerged. However, clinical studies on it...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1421103/full |
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| author | Pin-Chen Liu Pin-Chen Liu Kendy Tzu-yun Teng Kendy Tzu-yun Teng Tsia-Lu Lin Chi-Hsuan Sung Tsun-Li Cheng Chi-Chung Chou Chi-Chung Chou |
| author_facet | Pin-Chen Liu Pin-Chen Liu Kendy Tzu-yun Teng Kendy Tzu-yun Teng Tsia-Lu Lin Chi-Hsuan Sung Tsun-Li Cheng Chi-Chung Chou Chi-Chung Chou |
| author_sort | Pin-Chen Liu |
| collection | DOAJ |
| description | IntroductionCurrent point-of-care testing for canine-specific pancreatic lipase (CPL) provides semi-quantitative measurements with binary results. Recently, a commercial point-of-care testing method (Vcheck CPL) that offers quantitative measurement of CPL has emerged. However, clinical studies on its value (or utility) are limited. Therefore, this study aimed to evaluate the clinical utility of this commercial point-of-care CPL in diagnosing dogs with suspected acute pancreatitis and to assess its correlation with a commercial semi-quantitative test and other clinicopathological variables.MethodsA prospective observational study included 33 dogs with suspected acute pancreatitis and 20 clinically healthy dogs. Serum Vcheck CPL and SNAP ® cPL were tested, and clinical consensus scores were determined by 5 internists. Eleven dogs with suspected acute pancreatitis underwent follow-up testing during hospitalization. The intra-class correlation coefficient (ICC) was used for statistical analysis to assess the agreement between assays and the internists’ consensus score.ResultsDogs with suspected acute pancreatitis had significantly higher serum Vcheck CPL (median: 843 μg/L, range: 77–2001, p < 0.0001) than healthy control dogs (median: 94 μg/L, range: 49–294). By day 3 of hospitalization, serum Vcheck CPL had significantly decreased in dogs with suspected acute pancreatitis compared to day 1. The ICC score between the clinical consensus score, Vcheck CPL, and SNAP ® cPL was 0.75, indicating good agreement. Serum Vcheck CPL concentration was significantly correlated with serum concentrations of amylase, lipase, creatinine, ALP, and CRP.DiscussionThis study found good agreement between Vcheck CPL and SNAP ® cPL. This quantitative Vcheck CPL testing could serve as an adjunctive tool in diagnosing dogs with acute pancreatitis. |
| format | Article |
| id | doaj-art-3ca9c23e72c74e3080e617943c97b604 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-3ca9c23e72c74e3080e617943c97b6042025-02-12T07:25:31ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011210.3389/fvets.2025.14211031421103Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogsPin-Chen Liu0Pin-Chen Liu1Kendy Tzu-yun Teng2Kendy Tzu-yun Teng3Tsia-Lu Lin4Chi-Hsuan Sung5Tsun-Li Cheng6Chi-Chung Chou7Chi-Chung Chou8Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanVeterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanThe iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanVeterinary Medical Teaching Hospital, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, TaiwanDepartment and Graduate Institute of Pharmacology, National Defense Medical Center, Taipei, TaiwanIntroductionCurrent point-of-care testing for canine-specific pancreatic lipase (CPL) provides semi-quantitative measurements with binary results. Recently, a commercial point-of-care testing method (Vcheck CPL) that offers quantitative measurement of CPL has emerged. However, clinical studies on its value (or utility) are limited. Therefore, this study aimed to evaluate the clinical utility of this commercial point-of-care CPL in diagnosing dogs with suspected acute pancreatitis and to assess its correlation with a commercial semi-quantitative test and other clinicopathological variables.MethodsA prospective observational study included 33 dogs with suspected acute pancreatitis and 20 clinically healthy dogs. Serum Vcheck CPL and SNAP ® cPL were tested, and clinical consensus scores were determined by 5 internists. Eleven dogs with suspected acute pancreatitis underwent follow-up testing during hospitalization. The intra-class correlation coefficient (ICC) was used for statistical analysis to assess the agreement between assays and the internists’ consensus score.ResultsDogs with suspected acute pancreatitis had significantly higher serum Vcheck CPL (median: 843 μg/L, range: 77–2001, p < 0.0001) than healthy control dogs (median: 94 μg/L, range: 49–294). By day 3 of hospitalization, serum Vcheck CPL had significantly decreased in dogs with suspected acute pancreatitis compared to day 1. The ICC score between the clinical consensus score, Vcheck CPL, and SNAP ® cPL was 0.75, indicating good agreement. Serum Vcheck CPL concentration was significantly correlated with serum concentrations of amylase, lipase, creatinine, ALP, and CRP.DiscussionThis study found good agreement between Vcheck CPL and SNAP ® cPL. This quantitative Vcheck CPL testing could serve as an adjunctive tool in diagnosing dogs with acute pancreatitis.https://www.frontiersin.org/articles/10.3389/fvets.2025.1421103/fullacute pancreatitispancreatic lipase immunoreactivitylipaseamylaseinter-class correlation coefficientpoint-of-care |
| spellingShingle | Pin-Chen Liu Pin-Chen Liu Kendy Tzu-yun Teng Kendy Tzu-yun Teng Tsia-Lu Lin Chi-Hsuan Sung Tsun-Li Cheng Chi-Chung Chou Chi-Chung Chou Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs Frontiers in Veterinary Science acute pancreatitis pancreatic lipase immunoreactivity lipase amylase inter-class correlation coefficient point-of-care |
| title | Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| title_full | Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| title_fullStr | Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| title_full_unstemmed | Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| title_short | Assessment of point-of-care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| title_sort | assessment of point of care quantitative serum canine pancreatic lipase testing for diagnosing acute pancreatitis in dogs |
| topic | acute pancreatitis pancreatic lipase immunoreactivity lipase amylase inter-class correlation coefficient point-of-care |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1421103/full |
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