Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7
Protecting and balancing the intestinal flora is important for protection body against many diseases. It is important to develop probiotics, which are an important part of the flora balance, and prebiotic agents that encourage the development of probiotics. The study aimed t...
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College of Medicine, Al-Nahrain University
2025-03-01
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| Series: | Baghdad Journal of Biochemistry and Applied Biological Sciences |
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| Online Access: | https://www.bjbabs.org/index.php/bjbabs/article/view/283 |
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| author | ALİ SAĞLAM Meltem AŞAN ÖZÜSAĞLAM |
| author_facet | ALİ SAĞLAM Meltem AŞAN ÖZÜSAĞLAM |
| author_sort | ALİ SAĞLAM |
| collection | DOAJ |
| description | Protecting and balancing the intestinal flora is important for protection body against many diseases. It is important to develop probiotics, which are an important part of the flora balance, and prebiotic agents that encourage the development of probiotics. The study aimed to determine the potential use of the ethanol extract obtained from Chokeberry fruits with probiotic candidate Limosilactobacillus fermentumMA-7 as natural additive agents in the pharmaceutical and food industries.The biological activityof the extract was determined against probiotic candidate lactic acid bacteria (LAB) strains isolated from human breast milk. First, the inhibitory activity of the extract was determined using the disc diffusion method against five different LABs. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the extract on the LABs were determined using the micro-dilution method. Finally, the biological activity of the extract on L. fermentumMA-7 at concentrations of 50 and 100 mg/ml was determined using the macro-dilution method.The extract showed antibacterial activity only against Streptococcus thermophilusMAS-1, with an inhibition zone diameter of 6.43 mm. MIC and MBC values of the extract were found as 12.5 mg/ml ->50 mg/ml and 25 mg/ml ->50 mg/ml, respectively. The stimulatory activity of the extract on L. fermentumMA-7 was obtained using macro-dilution method. The number of viable cells was determined after 0, 24 and 48 hours. A concentration of 50 mg/mlextract promoted the growth of L. fermentumMA-7 at 24 and 48 hours. 100 mg/ml extract concentration inhibited the growth of L. fermentumMA-7 after 24 hours but increased its growth after 48 hour compared to 24th hour. It has been shown that Chokeberry fruit ethanol extract at appropriate concentrations can be an alternative as a natural stimulant for L. fermentumMA-7 to support its development in the pharmaceutical industry and as a natural additive in the food industries. |
| format | Article |
| id | doaj-art-3ca7ba4215964c32ac70bcf46b85faa6 |
| institution | OA Journals |
| issn | 2706-9915 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | College of Medicine, Al-Nahrain University |
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| series | Baghdad Journal of Biochemistry and Applied Biological Sciences |
| spelling | doaj-art-3ca7ba4215964c32ac70bcf46b85faa62025-08-20T02:27:49ZengCollege of Medicine, Al-Nahrain UniversityBaghdad Journal of Biochemistry and Applied Biological Sciences2706-99152025-03-01611910.47419/bjbabs.v6i1.283Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7ALİ SAĞLAM0https://orcid.org/0000-0003-4010-8465Meltem AŞAN ÖZÜSAĞLAM1https://orcid.org/0000-0002-3638-1306Department of Molecular Biology and Genetic, Faculty of Science and Letters, University of Aksaray, Aksaray, TurkeyDepartment of Molecular Biology and Genetic, Faculty of Science and Letters, University of Aksaray, Aksaray, TurkeyProtecting and balancing the intestinal flora is important for protection body against many diseases. It is important to develop probiotics, which are an important part of the flora balance, and prebiotic agents that encourage the development of probiotics. The study aimed to determine the potential use of the ethanol extract obtained from Chokeberry fruits with probiotic candidate Limosilactobacillus fermentumMA-7 as natural additive agents in the pharmaceutical and food industries.The biological activityof the extract was determined against probiotic candidate lactic acid bacteria (LAB) strains isolated from human breast milk. First, the inhibitory activity of the extract was determined using the disc diffusion method against five different LABs. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the extract on the LABs were determined using the micro-dilution method. Finally, the biological activity of the extract on L. fermentumMA-7 at concentrations of 50 and 100 mg/ml was determined using the macro-dilution method.The extract showed antibacterial activity only against Streptococcus thermophilusMAS-1, with an inhibition zone diameter of 6.43 mm. MIC and MBC values of the extract were found as 12.5 mg/ml ->50 mg/ml and 25 mg/ml ->50 mg/ml, respectively. The stimulatory activity of the extract on L. fermentumMA-7 was obtained using macro-dilution method. The number of viable cells was determined after 0, 24 and 48 hours. A concentration of 50 mg/mlextract promoted the growth of L. fermentumMA-7 at 24 and 48 hours. 100 mg/ml extract concentration inhibited the growth of L. fermentumMA-7 after 24 hours but increased its growth after 48 hour compared to 24th hour. It has been shown that Chokeberry fruit ethanol extract at appropriate concentrations can be an alternative as a natural stimulant for L. fermentumMA-7 to support its development in the pharmaceutical industry and as a natural additive in the food industries.https://www.bjbabs.org/index.php/bjbabs/article/view/283antimicrobial activityaronia melanocarpaextractlactic acid bacterialiving cell |
| spellingShingle | ALİ SAĞLAM Meltem AŞAN ÖZÜSAĞLAM Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 Baghdad Journal of Biochemistry and Applied Biological Sciences antimicrobial activity aronia melanocarpa extract lactic acid bacteria living cell |
| title | Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 |
| title_full | Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 |
| title_fullStr | Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 |
| title_full_unstemmed | Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 |
| title_short | Chokeberry Extract: Inhibitory Activity on Some Lactic Acid Bacteria and the Growth Stimulative Effect on Limosilactobacillus fermentum MA-7 |
| title_sort | chokeberry extract inhibitory activity on some lactic acid bacteria and the growth stimulative effect on limosilactobacillus fermentum ma 7 |
| topic | antimicrobial activity aronia melanocarpa extract lactic acid bacteria living cell |
| url | https://www.bjbabs.org/index.php/bjbabs/article/view/283 |
| work_keys_str_mv | AT alisaglam chokeberryextractinhibitoryactivityonsomelacticacidbacteriaandthegrowthstimulativeeffectonlimosilactobacillusfermentumma7 AT meltemasanozusaglam chokeberryextractinhibitoryactivityonsomelacticacidbacteriaandthegrowthstimulativeeffectonlimosilactobacillusfermentumma7 |