A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma
Papillary thyroid carcinoma (PTC) is the most frequent histological type of differentiated thyroid carcinoma. Long noncoding RNAs (lncRNAs) have been widely reported to play a key role in human malignancies, and PTC is included. This study aimed to find out the functions and mechanism of lncRNA LINC...
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De Gruyter
2021-12-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2021-0389 |
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| author | Yang Yarong Hua Wenjuan Zeng Mei Yu Liling Zhang Baijun Wen Liming |
| author_facet | Yang Yarong Hua Wenjuan Zeng Mei Yu Liling Zhang Baijun Wen Liming |
| author_sort | Yang Yarong |
| collection | DOAJ |
| description | Papillary thyroid carcinoma (PTC) is the most frequent histological type of differentiated thyroid carcinoma. Long noncoding RNAs (lncRNAs) have been widely reported to play a key role in human malignancies, and PTC is included. This study aimed to find out the functions and mechanism of lncRNA LINC00475 in PTC. LINC00475 was upregulated in PTC cells and was mainly located in the cytoplasm according to reverse-transcription polymerase chain reaction analyses and subcellular fractionation assays. As shown by cell counting kit-8 assays, ethynyl deoxyuridine incorporation assays, wound healing assays, and transwell assays, LINC00475 knockdown suppressed cell viability, proliferation, migration, and invasion. Mechanistically, LINC00475 upregulated the expression of messenger RNA zinc finger CCHC-type containing 12 (ZCCHC12) by binding to miR-376c-3p. ZCCHC12 was a direct target gene of miR-376c-3p in PTC cells. The relationship between miR-376c-3p and LINC00475 (or ZCCHC12) in PTC cells was probed by luciferase reporter assays, RNA pulldown assays, and RNA immunoprecipitation assays. In addition, both mRNA and protein levels of ZCCHC12 were downregulated due to miR-376c-3p overexpression or LINC00475 silencing. ZCCHC12 overexpression partially reversed the suppressive effect of LINC00475 knockdown on malignant behaviors of PTC cells. In conclusion, LINC00475 promotes PTC cell proliferation, migration, and invasion by upregulating ZCCHC12 via the interaction with miR-376c-3p. |
| format | Article |
| id | doaj-art-3ca21a72befe469fbe73990be0b37c84 |
| institution | DOAJ |
| issn | 2391-5463 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | De Gruyter |
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| series | Open Medicine |
| spelling | doaj-art-3ca21a72befe469fbe73990be0b37c842025-08-20T03:06:37ZengDe GruyterOpen Medicine2391-54632021-12-01171223310.1515/med-2021-0389A ceRNA network mediated by LINC00475 in papillary thyroid carcinomaYang Yarong0Hua Wenjuan1Zeng Mei2Yu Liling3Zhang Baijun4Wen Liming5Department of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaDepartment of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaDepartment of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaDepartment of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaDepartment of Nuclear Medicine, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaDepartment of Anesthesiology, Wuhan Fourth Hospital/Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, ChinaPapillary thyroid carcinoma (PTC) is the most frequent histological type of differentiated thyroid carcinoma. Long noncoding RNAs (lncRNAs) have been widely reported to play a key role in human malignancies, and PTC is included. This study aimed to find out the functions and mechanism of lncRNA LINC00475 in PTC. LINC00475 was upregulated in PTC cells and was mainly located in the cytoplasm according to reverse-transcription polymerase chain reaction analyses and subcellular fractionation assays. As shown by cell counting kit-8 assays, ethynyl deoxyuridine incorporation assays, wound healing assays, and transwell assays, LINC00475 knockdown suppressed cell viability, proliferation, migration, and invasion. Mechanistically, LINC00475 upregulated the expression of messenger RNA zinc finger CCHC-type containing 12 (ZCCHC12) by binding to miR-376c-3p. ZCCHC12 was a direct target gene of miR-376c-3p in PTC cells. The relationship between miR-376c-3p and LINC00475 (or ZCCHC12) in PTC cells was probed by luciferase reporter assays, RNA pulldown assays, and RNA immunoprecipitation assays. In addition, both mRNA and protein levels of ZCCHC12 were downregulated due to miR-376c-3p overexpression or LINC00475 silencing. ZCCHC12 overexpression partially reversed the suppressive effect of LINC00475 knockdown on malignant behaviors of PTC cells. In conclusion, LINC00475 promotes PTC cell proliferation, migration, and invasion by upregulating ZCCHC12 via the interaction with miR-376c-3p.https://doi.org/10.1515/med-2021-0389papillary thyroid carcinomazcchc12mir-376c-3plinc00475competing endogenous rna |
| spellingShingle | Yang Yarong Hua Wenjuan Zeng Mei Yu Liling Zhang Baijun Wen Liming A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma Open Medicine papillary thyroid carcinoma zcchc12 mir-376c-3p linc00475 competing endogenous rna |
| title | A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma |
| title_full | A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma |
| title_fullStr | A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma |
| title_full_unstemmed | A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma |
| title_short | A ceRNA network mediated by LINC00475 in papillary thyroid carcinoma |
| title_sort | cerna network mediated by linc00475 in papillary thyroid carcinoma |
| topic | papillary thyroid carcinoma zcchc12 mir-376c-3p linc00475 competing endogenous rna |
| url | https://doi.org/10.1515/med-2021-0389 |
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