Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy
Traditional food habits followed by indigenous populations have a huge potential in providing nutrition to the world community aided with nutritional supplements. The phylum mollusca can provide alternative supplements for food and biochemical potentials. In the current investigation, the hemolymph...
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Elsevier
2025-10-01
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| Series: | Kuwait Journal of Science |
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S2307410825000999 |
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| author | Minati Soren Sambit Kumar Mishra Awdhesh Kumar Mishra Pradipta Ranjan Rauta Jibanjyoti Panda Sarvesh Rustagi Seydur Rahman Yugal Kishore Mohanta Debasis Nayak |
| author_facet | Minati Soren Sambit Kumar Mishra Awdhesh Kumar Mishra Pradipta Ranjan Rauta Jibanjyoti Panda Sarvesh Rustagi Seydur Rahman Yugal Kishore Mohanta Debasis Nayak |
| author_sort | Minati Soren |
| collection | DOAJ |
| description | Traditional food habits followed by indigenous populations have a huge potential in providing nutrition to the world community aided with nutritional supplements. The phylum mollusca can provide alternative supplements for food and biochemical potentials. In the current investigation, the hemolymph of Pomacea canaliculata, Achatina fulica, and Lamellidense marginalis was collected through the heart puncture method and they were characterized through fourier transformation infrared spectroscopy (FTIR), gas chromatography-mass spectroscopy (GC-MS), Inductively coupled plasma atomic emission spectroscopy (ICP-OES), antioxidant activity, hemolysis, antimicrobial and antibiofilm activity. The collected hemolymph demonstrated a prominent 65 kDa protein band in Sodium Dodecyl Sulfate- Poly Acrylamide Gel Electrophoresis (SDS-PAGE). The hemolymph samples exhibited strong antioxidant activity against standard free radicals. They were found to be highly haemocompatible through hemolysis and hemagglutination assay. The hemolymph exhibited antimicrobial activity against the pathogenic Escherichia coli, Pseudomonas aeruginosa and Vibrio cholerae. All the test samples demonstrated high biofilm inhibiting activity through tube assay and Congo red assay. The hemolymph extracts confirmed dose dependent cell toxicity against the A431 skin carcinoma cells. Hence, this study provides an insight into the potential application of the hemolymph in various biomedical applications, which can be validated through future in vitro experimentations. |
| format | Article |
| id | doaj-art-3ca20da7f17f4d159e411ac03f3e47ec |
| institution | Kabale University |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kuwait Journal of Science |
| spelling | doaj-art-3ca20da7f17f4d159e411ac03f3e47ec2025-08-20T03:24:25ZengElsevierKuwait Journal of Science2307-41082307-41162025-10-01524100455https://doi.org/10.1016/j.kjs.2025.100455Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacyMinati Soren0Sambit Kumar Mishra1Awdhesh Kumar Mishra2Pradipta Ranjan Rauta3Jibanjyoti Panda4Sarvesh Rustagi5Seydur Rahman6https://orcid.org/0000-0002-9151-5434Yugal Kishore Mohanta7Debasis Nayak8https://orcid.org/0000-0003-3333-1300Bioresource and Traditional Knowledge Laboratory, Department of Wildlife and Biodiversity Conservation, Maharaja Sriram Chandra Bhanja Deo University, Sriram Chandra Vihar, Takatpur, Baripada, Mayurbhanj, Odisha, 757003, IndiaBioresource and Traditional Knowledge Laboratory, Department of Wildlife and Biodiversity Conservation, Maharaja Sriram Chandra Bhanja Deo University, Sriram Chandra Vihar, Takatpur, Baripada, Mayurbhanj, Odisha, 757003, IndiaDepartment of Biotechnology, Yeungnam University, Gyeongsan 38541, Gyeongsangbuk-do, Republic of KoreaDepartment of Biological Sciences, AIPH University, Bhubaneswar, Odisha, 754001, IndiaBioresource and Traditional Knowledge Laboratory, Department of Wildlife and Biodiversity Conservation, Maharaja Sriram Chandra Bhanja Deo University, Sriram Chandra Vihar, Takatpur, Baripada, Mayurbhanj, Odisha, 757003, IndiaDepartment of Food Technology, School of Agriculture, Maya Devi University, Dehradun, Uttarakhand, 248011, IndiaDepartment of Zoology, Faculty of Science, Assam down town University, Sankar Madhab Path, Gandhi Nagar, Panikhaiti , Guwahati, Assam, 781026, IndiaNanobiotechnology and Translational Knowledge Laboratory, Department of Applied Biology, School of Biological Sciences, University of Science and Technology Meghalaya (USTM), Baridua, Ri-Bhoi, Meghalaya, 793101, IndiaBioresource and Traditional Knowledge Laboratory, Department of Wildlife and Biodiversity Conservation, Maharaja Sriram Chandra Bhanja Deo University, Sriram Chandra Vihar, Takatpur, Baripada, Mayurbhanj, Odisha, 757003, IndiaTraditional food habits followed by indigenous populations have a huge potential in providing nutrition to the world community aided with nutritional supplements. The phylum mollusca can provide alternative supplements for food and biochemical potentials. In the current investigation, the hemolymph of Pomacea canaliculata, Achatina fulica, and Lamellidense marginalis was collected through the heart puncture method and they were characterized through fourier transformation infrared spectroscopy (FTIR), gas chromatography-mass spectroscopy (GC-MS), Inductively coupled plasma atomic emission spectroscopy (ICP-OES), antioxidant activity, hemolysis, antimicrobial and antibiofilm activity. The collected hemolymph demonstrated a prominent 65 kDa protein band in Sodium Dodecyl Sulfate- Poly Acrylamide Gel Electrophoresis (SDS-PAGE). The hemolymph samples exhibited strong antioxidant activity against standard free radicals. They were found to be highly haemocompatible through hemolysis and hemagglutination assay. The hemolymph exhibited antimicrobial activity against the pathogenic Escherichia coli, Pseudomonas aeruginosa and Vibrio cholerae. All the test samples demonstrated high biofilm inhibiting activity through tube assay and Congo red assay. The hemolymph extracts confirmed dose dependent cell toxicity against the A431 skin carcinoma cells. Hence, this study provides an insight into the potential application of the hemolymph in various biomedical applications, which can be validated through future in vitro experimentations.https://www.sciencedirect.com/science/article/pii/S2307410825000999molluscahemolymphproximate componentsantibiofilmmayurbhanjtribal food |
| spellingShingle | Minati Soren Sambit Kumar Mishra Awdhesh Kumar Mishra Pradipta Ranjan Rauta Jibanjyoti Panda Sarvesh Rustagi Seydur Rahman Yugal Kishore Mohanta Debasis Nayak Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy Kuwait Journal of Science mollusca hemolymph proximate components antibiofilm mayurbhanj tribal food |
| title | Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy |
| title_full | Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy |
| title_fullStr | Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy |
| title_full_unstemmed | Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy |
| title_short | Assessment of toxicity profile of Mollusca hemolymph: Insight into its potential biocompatibility and anti-biofilm efficacy |
| title_sort | assessment of toxicity profile of mollusca hemolymph insight into its potential biocompatibility and anti biofilm efficacy |
| topic | mollusca hemolymph proximate components antibiofilm mayurbhanj tribal food |
| url | https://www.sciencedirect.com/science/article/pii/S2307410825000999 |
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