A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study
Objectives: 1. Assess serum biomarker responses of the anterior cruciate ligament reconstructed (ACLR) knee compared to the non-injured contralateral knee in a lateral tilt paradigm. 2. Determine the relationship between kinematics at the ACLR knee and serum biomarker responses. Method: Cross-sequen...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Osteoarthritis and Cartilage Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266591312500055X |
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| author | Prakash Jayabalan Rose Darcy Vikram Darbhe Zander Neuville Vehniah Tjong Levi Hargrove Leah Welty Thomas Schnitzer |
| author_facet | Prakash Jayabalan Rose Darcy Vikram Darbhe Zander Neuville Vehniah Tjong Levi Hargrove Leah Welty Thomas Schnitzer |
| author_sort | Prakash Jayabalan |
| collection | DOAJ |
| description | Objectives: 1. Assess serum biomarker responses of the anterior cruciate ligament reconstructed (ACLR) knee compared to the non-injured contralateral knee in a lateral tilt paradigm. 2. Determine the relationship between kinematics at the ACLR knee and serum biomarker responses. Method: Cross-sequential study. 16 participants 2–7 years post-ACLR, undertook two 30 min walking sessions on a 10° angular tilted treadmill towards the ACLR knee and/or contralateral non-injured knee. Serum collected at baseline and after 30 min of walking was tested for biomarkers associated with osteoarthritis (Cartilage oligomeric matrix protein [COMP]), C-terminal cross-linked telopeptides of type II collagen, matrix metalloproteinase-3,-13, ADAM a disintegrin and metalloproteinase with thrombospondin motifs 4-,-5 (ADAMTS-4,-5), interleukin-1β,-6,-8 and RANTES (regulated on activation, normal T-cell expressed and secreted). Kinematic measurements were also taken. Results: When tilted towards the ACLR knee, there was a significant increase in COMP compared to baseline (mean 41.1 ng/mL (95%CI:13.8,68.4), not observed when tilted towards the contralateral knee. There was a significant correlation between change in COMP concentration and change in knee adduction/abduction angle (r = −0.58,p = 0.02). There were no relationships to kinematics and the other biomarkers. Conclusions: The tilt paradigm identified a differential response of a serum mechanosensitive marker (COMP) when testing an ACLR and contralateral knee. There was also a significant relationship between COMP response to tilt walking and the change in knee adduction angle of the ACLR knee. This paradigm could be an aid to provide individual knee biomarker response to a mechanical stress in asymptomatic individuals at risk of developing post-traumatic osteoarthritis at least 2 years post-ACLR. |
| format | Article |
| id | doaj-art-3c9b2a90a8bb4c028ba250359f0ab048 |
| institution | OA Journals |
| issn | 2665-9131 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Osteoarthritis and Cartilage Open |
| spelling | doaj-art-3c9b2a90a8bb4c028ba250359f0ab0482025-08-20T02:17:14ZengElsevierOsteoarthritis and Cartilage Open2665-91312025-09-017310061910.1016/j.ocarto.2025.100619A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot studyPrakash Jayabalan0Rose Darcy1Vikram Darbhe2Zander Neuville3Vehniah Tjong4Levi Hargrove5Leah Welty6Thomas Schnitzer7Shirley Ryan AbilityLab, USA; Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Corresponding author. Shirley Ryan AbilityLab, USA.Shirley Ryan AbilityLab, USA; Northwestern University Feinberg School of Medicine, Chicago, IL, USAShirley Ryan AbilityLab, USANorthwestern University Feinberg School of Medicine, Chicago, IL, USANorthwestern University Feinberg School of Medicine, Chicago, IL, USAShirley Ryan AbilityLab, USA; Northwestern University Feinberg School of Medicine, Chicago, IL, USANorthwestern University Feinberg School of Medicine, Chicago, IL, USANorthwestern University Feinberg School of Medicine, Chicago, IL, USAObjectives: 1. Assess serum biomarker responses of the anterior cruciate ligament reconstructed (ACLR) knee compared to the non-injured contralateral knee in a lateral tilt paradigm. 2. Determine the relationship between kinematics at the ACLR knee and serum biomarker responses. Method: Cross-sequential study. 16 participants 2–7 years post-ACLR, undertook two 30 min walking sessions on a 10° angular tilted treadmill towards the ACLR knee and/or contralateral non-injured knee. Serum collected at baseline and after 30 min of walking was tested for biomarkers associated with osteoarthritis (Cartilage oligomeric matrix protein [COMP]), C-terminal cross-linked telopeptides of type II collagen, matrix metalloproteinase-3,-13, ADAM a disintegrin and metalloproteinase with thrombospondin motifs 4-,-5 (ADAMTS-4,-5), interleukin-1β,-6,-8 and RANTES (regulated on activation, normal T-cell expressed and secreted). Kinematic measurements were also taken. Results: When tilted towards the ACLR knee, there was a significant increase in COMP compared to baseline (mean 41.1 ng/mL (95%CI:13.8,68.4), not observed when tilted towards the contralateral knee. There was a significant correlation between change in COMP concentration and change in knee adduction/abduction angle (r = −0.58,p = 0.02). There were no relationships to kinematics and the other biomarkers. Conclusions: The tilt paradigm identified a differential response of a serum mechanosensitive marker (COMP) when testing an ACLR and contralateral knee. There was also a significant relationship between COMP response to tilt walking and the change in knee adduction angle of the ACLR knee. This paradigm could be an aid to provide individual knee biomarker response to a mechanical stress in asymptomatic individuals at risk of developing post-traumatic osteoarthritis at least 2 years post-ACLR.http://www.sciencedirect.com/science/article/pii/S266591312500055XKneePost-traumatic osteoarthritisBiomarkersBiomechanics |
| spellingShingle | Prakash Jayabalan Rose Darcy Vikram Darbhe Zander Neuville Vehniah Tjong Levi Hargrove Leah Welty Thomas Schnitzer A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study Osteoarthritis and Cartilage Open Knee Post-traumatic osteoarthritis Biomarkers Biomechanics |
| title | A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study |
| title_full | A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study |
| title_fullStr | A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study |
| title_full_unstemmed | A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study |
| title_short | A novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction: A pilot study |
| title_sort | novel mechanosensitive stress test in individuals following anterior cruciate ligament reconstruction a pilot study |
| topic | Knee Post-traumatic osteoarthritis Biomarkers Biomechanics |
| url | http://www.sciencedirect.com/science/article/pii/S266591312500055X |
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