Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation

Abstract This study explores the encapsulation efficiency of composite nanofibers and nanoemulsions derived from chia seed mucilage (Salvia hispanica L.), a natural hydrocolloid with notable bioactive properties. Nanofibers, known for their high surface area and stability, and nanoemulsions, recogni...

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Main Authors: Shirin Rashnoei, Mozhgan Shahamirian, Sedigheh Yazdanpanah, Elham Ansarifar
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-87703-3
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author Shirin Rashnoei
Mozhgan Shahamirian
Sedigheh Yazdanpanah
Elham Ansarifar
author_facet Shirin Rashnoei
Mozhgan Shahamirian
Sedigheh Yazdanpanah
Elham Ansarifar
author_sort Shirin Rashnoei
collection DOAJ
description Abstract This study explores the encapsulation efficiency of composite nanofibers and nanoemulsions derived from chia seed mucilage (Salvia hispanica L.), a natural hydrocolloid with notable bioactive properties. Nanofibers, known for their high surface area and stability, and nanoemulsions, recognized for their enhanced bioavailability, were utilized to encapsulate Bacillus coagulans. Chia mucilage demonstrated a DPPH radical scavenging activity of 67.88% at 350 µg/mL and moderate antimicrobial effects, with inhibition zones of 9 mm and 6 mm against Staphylococcus aureus and Escherichia coli, respectively. Nanofibers prepared with 2–3% mucilage showed uniform morphology (diameters: 12.36–26.24 μm) and significantly higher encapsulation efficiency (93.90 $$\:\pm\:$$ 2.1) (p < 0.05) than nanoemulsions (88.33 $$\:\pm\:$$ 2). FTIR analysis confirmed the successful encapsulation of Bacillus coagulans, with characteristic peaks in the ranges of 1500–1600 cm⁻¹, 2800–3000 cm⁻¹, and 3200–3500 cm⁻¹. These findings suggest that chia seed mucilage-based nanofibers offer a stable and efficient platform for probiotic delivery, with promising applications in functional foods, pharmaceuticals, and cosmetics.
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spelling doaj-art-3c9892ee79b744cc93ab06a76a428eef2025-08-20T02:25:35ZengNature PortfolioScientific Reports2045-23222025-04-0115111410.1038/s41598-025-87703-3Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans EncapsulationShirin Rashnoei0Mozhgan Shahamirian1Sedigheh Yazdanpanah2Elham Ansarifar3Department of Food Science and Technology, Sarvestan Branch, Islamic Azad UniversityDepartment of Chemistry, Faculty of Science, Sarvestan Branch, Islamic Azad UniversityDepartment of Food Science and Technology, Kazerun Branch, Islamic Azad UniversityDepartment of Pubic Health, School of Health, Birjand University of Medical ScienceAbstract This study explores the encapsulation efficiency of composite nanofibers and nanoemulsions derived from chia seed mucilage (Salvia hispanica L.), a natural hydrocolloid with notable bioactive properties. Nanofibers, known for their high surface area and stability, and nanoemulsions, recognized for their enhanced bioavailability, were utilized to encapsulate Bacillus coagulans. Chia mucilage demonstrated a DPPH radical scavenging activity of 67.88% at 350 µg/mL and moderate antimicrobial effects, with inhibition zones of 9 mm and 6 mm against Staphylococcus aureus and Escherichia coli, respectively. Nanofibers prepared with 2–3% mucilage showed uniform morphology (diameters: 12.36–26.24 μm) and significantly higher encapsulation efficiency (93.90 $$\:\pm\:$$ 2.1) (p < 0.05) than nanoemulsions (88.33 $$\:\pm\:$$ 2). FTIR analysis confirmed the successful encapsulation of Bacillus coagulans, with characteristic peaks in the ranges of 1500–1600 cm⁻¹, 2800–3000 cm⁻¹, and 3200–3500 cm⁻¹. These findings suggest that chia seed mucilage-based nanofibers offer a stable and efficient platform for probiotic delivery, with promising applications in functional foods, pharmaceuticals, and cosmetics.https://doi.org/10.1038/s41598-025-87703-3Bacillus coagulansChia seedNanofibersNanoemulsionElectrospun
spellingShingle Shirin Rashnoei
Mozhgan Shahamirian
Sedigheh Yazdanpanah
Elham Ansarifar
Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
Scientific Reports
Bacillus coagulans
Chia seed
Nanofibers
Nanoemulsion
Electrospun
title Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
title_full Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
title_fullStr Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
title_full_unstemmed Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
title_short Development and evaluation of Chia seed-based nanofibers and nanoemulsions for Bacillus coagulans Encapsulation
title_sort development and evaluation of chia seed based nanofibers and nanoemulsions for bacillus coagulans encapsulation
topic Bacillus coagulans
Chia seed
Nanofibers
Nanoemulsion
Electrospun
url https://doi.org/10.1038/s41598-025-87703-3
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AT mozhganshahamirian developmentandevaluationofchiaseedbasednanofibersandnanoemulsionsforbacilluscoagulansencapsulation
AT sedighehyazdanpanah developmentandevaluationofchiaseedbasednanofibersandnanoemulsionsforbacilluscoagulansencapsulation
AT elhamansarifar developmentandevaluationofchiaseedbasednanofibersandnanoemulsionsforbacilluscoagulansencapsulation