Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors

Purpose: to compare the regenerative activity of benign and malignant conjunctival tumors.Material and methods. The study was performed on 86 tumor biopsies from patients with a clinically diagnosed conjunctival neoplasm. Healthy tissues from the same eye were taken as a control sample. The optical...

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Main Authors: S. V. Saakyan, D. A. Skladnev, A. P. Alekseeva, V. V. Sorokin, O. V. Beznos
Format: Article
Language:Russian
Published: Real Time Ltd 2023-07-01
Series:Российский офтальмологический журнал
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Online Access:https://roj.igb.ru/jour/article/view/1243
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author S. V. Saakyan
D. A. Skladnev
A. P. Alekseeva
V. V. Sorokin
O. V. Beznos
author_facet S. V. Saakyan
D. A. Skladnev
A. P. Alekseeva
V. V. Sorokin
O. V. Beznos
author_sort S. V. Saakyan
collection DOAJ
description Purpose: to compare the regenerative activity of benign and malignant conjunctival tumors.Material and methods. The study was performed on 86 tumor biopsies from patients with a clinically diagnosed conjunctival neoplasm. Healthy tissues from the same eye were taken as a control sample. The optical density of the reaction mixture containing biogenic nanoparticles formed in the presence of conjunctival tumors and the corresponding paired healthy tissue samples from the control group was measured by spectrophotometry. The data obtained were later verified by a pathohistological analysis.Results. A significantly higher level of in situ formation of biogenic silver nanoparticles was recorded in the reaction mixture with malignant tumors of the conjunctiva (3.0 ± 1.1, (n = 32) compared to benign tumors (1.3 ± 0.2, n = 54). No differences were found between various types of benign tumors. In samples of malignant tumors, the indicators of recovery activity in melanoma (3.4 ± 1.0, n = 14) and lymphoma (2.8 ± 1.0, n = 7) were significantly higher than in squamous cell carcinoma (2.0 ± 0.6, n = 11), but no significant differences were found between the two types of tumors.Conclusion. The method of spectrophotometric measurement of the regenerative activity of conjunctival tumors can be used in preoperative or intraoperative diagnostics thanks to the fact that the results can be obtained rapidly, which will help to quickly determine the extent of surgical intervention needed and optimize the treatment tactics.
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spelling doaj-art-3c8c90dd366d4c69900f82e9f62314da2025-08-20T03:39:28ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602023-07-0116211912310.21516/2072-0076-2023-16-2-119-123589Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumorsS. V. Saakyan0D. A. Skladnev1A. P. Alekseeva2V. V. Sorokin3O. V. Beznos4Helmholtz National Medical Research Center of Eye DiseasesResearch Center of Biotechnology, S.N.Vinogradsky Institute of Microbiology, RASHelmholtz National Medical Research Center of Eye DiseasesResearch Center of Biotechnology, S.N.Vinogradsky Institute of Microbiology, RASHelmholtz National Medical Research Center of Eye DiseasesPurpose: to compare the regenerative activity of benign and malignant conjunctival tumors.Material and methods. The study was performed on 86 tumor biopsies from patients with a clinically diagnosed conjunctival neoplasm. Healthy tissues from the same eye were taken as a control sample. The optical density of the reaction mixture containing biogenic nanoparticles formed in the presence of conjunctival tumors and the corresponding paired healthy tissue samples from the control group was measured by spectrophotometry. The data obtained were later verified by a pathohistological analysis.Results. A significantly higher level of in situ formation of biogenic silver nanoparticles was recorded in the reaction mixture with malignant tumors of the conjunctiva (3.0 ± 1.1, (n = 32) compared to benign tumors (1.3 ± 0.2, n = 54). No differences were found between various types of benign tumors. In samples of malignant tumors, the indicators of recovery activity in melanoma (3.4 ± 1.0, n = 14) and lymphoma (2.8 ± 1.0, n = 7) were significantly higher than in squamous cell carcinoma (2.0 ± 0.6, n = 11), but no significant differences were found between the two types of tumors.Conclusion. The method of spectrophotometric measurement of the regenerative activity of conjunctival tumors can be used in preoperative or intraoperative diagnostics thanks to the fact that the results can be obtained rapidly, which will help to quickly determine the extent of surgical intervention needed and optimize the treatment tactics.https://roj.igb.ru/jour/article/view/1243tumorconjunctivabiogenic nanoparticlesspectrophotometry
spellingShingle S. V. Saakyan
D. A. Skladnev
A. P. Alekseeva
V. V. Sorokin
O. V. Beznos
Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
Российский офтальмологический журнал
tumor
conjunctiva
biogenic nanoparticles
spectrophotometry
title Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
title_full Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
title_fullStr Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
title_full_unstemmed Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
title_short Possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
title_sort possibilities of spectrometric diagnostics of benign and malignant conjunctival tumors
topic tumor
conjunctiva
biogenic nanoparticles
spectrophotometry
url https://roj.igb.ru/jour/article/view/1243
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AT daskladnev possibilitiesofspectrometricdiagnosticsofbenignandmalignantconjunctivaltumors
AT apalekseeva possibilitiesofspectrometricdiagnosticsofbenignandmalignantconjunctivaltumors
AT vvsorokin possibilitiesofspectrometricdiagnosticsofbenignandmalignantconjunctivaltumors
AT ovbeznos possibilitiesofspectrometricdiagnosticsofbenignandmalignantconjunctivaltumors