Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria
Introduction: Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infecti...
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The Journal of Infection in Developing Countries
2020-02-01
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| Online Access: | https://jidc.org/index.php/journal/article/view/11746 |
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| author | Tolulope Funbi Jolaiya Muinah Adenike Fowora Charles Onyekwere Rose Ugiagbe Ifeanyi Ifeoma Agbo Olufunmilayo Lesi Dennis Amojuayi Ndububa Olusegun Adekanle Henry Akum Njom Ayodeji Idowu Isaac Adeyemi Adeleye Moses Bamidele Favor Ndidiamaka Ngoka Pia Fiona Palamides Stella Ifeanyi Smith |
| author_facet | Tolulope Funbi Jolaiya Muinah Adenike Fowora Charles Onyekwere Rose Ugiagbe Ifeanyi Ifeoma Agbo Olufunmilayo Lesi Dennis Amojuayi Ndububa Olusegun Adekanle Henry Akum Njom Ayodeji Idowu Isaac Adeyemi Adeleye Moses Bamidele Favor Ndidiamaka Ngoka Pia Fiona Palamides Stella Ifeanyi Smith |
| author_sort | Tolulope Funbi Jolaiya |
| collection | DOAJ |
| description |
Introduction: Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infections in the Nigerian population.
Methodology: Polymerase Chain Reaction was used to analyze a total of forty-nine H. pylori strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete dupA (G27 variant), jhp0917, jhp0918, other plasticity region genes jhp 914/917, jhp0914, jhp0940 and sigma factors.
Results: PCR results indicated that the prevalence of complete dupA (G27 variants), jhp0917, jhp0918 and other plasticity region genes jhp0914, jhp0914/0917 and jhp0940 in the H. pylori strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for rpoN, fliA and rpoD respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the H. pylori strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between jhp0917, jhp914/917 and G27 variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings.
Conclusion: These results suggest that the presence of jhp0917, jhp0914/917 and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with H. pylori infection.
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| format | Article |
| id | doaj-art-3c82fcaeb0dd4aaaabb85abb24776e08 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-3c82fcaeb0dd4aaaabb85abb24776e082025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-02-01140210.3855/jidc.11746Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from NigeriaTolulope Funbi Jolaiya0Muinah Adenike Fowora1Charles Onyekwere2Rose Ugiagbe3Ifeanyi Ifeoma Agbo4Olufunmilayo Lesi5Dennis Amojuayi Ndububa6Olusegun Adekanle7Henry Akum Njom8Ayodeji Idowu9Isaac Adeyemi Adeleye10Moses Bamidele11Favor Ndidiamaka Ngoka12Pia Fiona Palamides13Stella Ifeanyi Smith14Department of Microbiology, University of Lagos, Akoka, Lagos state, NigeriaMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, NigeriaDepartment of Medicine, Lagos State University Teaching Hospital, Ikeja, Lagos state, NigeriaDepartment of Medicine, University of Benin Teaching Hospital, Benin, Edo state, NigeriaDepartment of Medicine, University of Benin Teaching Hospital, Benin, Edo state, NigeriaDepartment of Medicine, Lagos University Teaching Hospital, Idi-Araba, Lagos state, NigeriaDepartment of Medicine, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Osun state, NigeriaDepartment of Medicine, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Osun state, NigeriaDepartment of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern cape, South AfricaDepartment of BiDepartment of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern cape, South Africaochemistry and Microbiology, University of Fort Hare, South AfricaDepartment of Microbiology, University of Lagos, Akoka, Lagos state, NigeriaMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, NigeriaMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, NigeriaChair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, GermanyMolecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria Introduction: Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infections in the Nigerian population. Methodology: Polymerase Chain Reaction was used to analyze a total of forty-nine H. pylori strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete dupA (G27 variant), jhp0917, jhp0918, other plasticity region genes jhp 914/917, jhp0914, jhp0940 and sigma factors. Results: PCR results indicated that the prevalence of complete dupA (G27 variants), jhp0917, jhp0918 and other plasticity region genes jhp0914, jhp0914/0917 and jhp0940 in the H. pylori strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for rpoN, fliA and rpoD respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the H. pylori strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between jhp0917, jhp914/917 and G27 variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings. Conclusion: These results suggest that the presence of jhp0917, jhp0914/917 and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with H. pylori infection. https://jidc.org/index.php/journal/article/view/11746Helicobacter pyloriSigma factorsPlasticity region genesDupATranscriptionPathology |
| spellingShingle | Tolulope Funbi Jolaiya Muinah Adenike Fowora Charles Onyekwere Rose Ugiagbe Ifeanyi Ifeoma Agbo Olufunmilayo Lesi Dennis Amojuayi Ndububa Olusegun Adekanle Henry Akum Njom Ayodeji Idowu Isaac Adeyemi Adeleye Moses Bamidele Favor Ndidiamaka Ngoka Pia Fiona Palamides Stella Ifeanyi Smith Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria Journal of Infection in Developing Countries Helicobacter pylori Sigma factors Plasticity region genes DupA Transcription Pathology |
| title | Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria |
| title_full | Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria |
| title_fullStr | Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria |
| title_full_unstemmed | Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria |
| title_short | Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria |
| title_sort | duodenal ulcer promoting gene dupa plasticity region genes and sigma factors in h pyloristrains from nigeria |
| topic | Helicobacter pylori Sigma factors Plasticity region genes DupA Transcription Pathology |
| url | https://jidc.org/index.php/journal/article/view/11746 |
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