Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster)
Introduction: Studies have highlighted the role of actin cables in embryonic scarless wound healing across various species. However, evidence for similar structures in adult animals remains lacking. Adult newts, known for their exceptional skin regeneration capabilities, are considered promising mod...
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Elsevier
2025-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320425000689 |
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| author | Yu Liou Nobuko Moritoki Tomoko Shindo Tatsuyuki Ishii Kazuo Kishi |
| author_facet | Yu Liou Nobuko Moritoki Tomoko Shindo Tatsuyuki Ishii Kazuo Kishi |
| author_sort | Yu Liou |
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| description | Introduction: Studies have highlighted the role of actin cables in embryonic scarless wound healing across various species. However, evidence for similar structures in adult animals remains lacking. Adult newts, known for their exceptional skin regeneration capabilities, are considered promising models for postnatal human studies. This study investigated actin fiber formation and alignment during re-epithelialization in the Japanese fire-bellied newt (Cynops pyrrhogaster). Methods: Full-thickness skin excisions were performed, and actin structures were analyzed using immunohistochemistry and electron microscopy. The role of actin in re-epithelialization was assessed by inhibiting its formation with cytochalasin B. Myosin, an interacting cytoskeletal molecule, was examined through immunohistochemistry, while E-cadherin, an adhesion molecule, was analyzed using both immunohistochemistry and electron microscopy. Results: Rather than an actin cable a mesh-like actin structure, termed the “actin mesh,” was identified via immunohistochemical analysis. The actin mesh developed alongside wound epidermis extension and disappeared following complete re-epithelialization. Inhibition of actin formation delayed re-epithelialization, although the overall healing process showed no significant difference from the control group. Immunohistochemistry revealed the presence of myosin II and E-cadherin alongside Filamentous actin. Electron microscopy further demonstrated actin-rich structures in the wound epidermis compared to normal skin and confirmed E-cadherin-mediated cell-cell adhesion in the wound area. Conclusions: The actin mesh plays a critical role in facilitating rapid re-epithelialization in adult newts, presenting a valuable model for studying scarless wound healing in adult organisms. The involvement of interacting molecules such as myosin and E-cadherin provides insights into the underlying mechanisms of this process. This model offers potential applications for addressing intractable wounds in humans. |
| format | Article |
| id | doaj-art-3c75de1203ae4a658f036a08b2f3c2bc |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Regenerative Therapy |
| spelling | doaj-art-3c75de1203ae4a658f036a08b2f3c2bc2025-08-20T01:51:16ZengElsevierRegenerative Therapy2352-32042025-06-012925426110.1016/j.reth.2025.03.014Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster)Yu Liou0Nobuko Moritoki1Tomoko Shindo2Tatsuyuki Ishii3Kazuo Kishi4Department of Medical Education, Linkou Chang Gung Memorial Hospital, No.5, Fusing St., Gueishan Township, Taoyuan County 333, TaiwanElectron Microscope Laboratory, Keio University School of Medicine, Shinanomachi 35, Tokyo 160-8582, JapanElectron Microscope Laboratory, Keio University School of Medicine, Shinanomachi 35, Tokyo 160-8582, JapanDepartment of Plastic and Reconstructive Surgery, Keio University School of Medicine, Shinanomachi 35, Tokyo 160-8582, Japan; Corresponding author.Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Shinanomachi 35, Tokyo 160-8582, Japan; Corresponding author.Introduction: Studies have highlighted the role of actin cables in embryonic scarless wound healing across various species. However, evidence for similar structures in adult animals remains lacking. Adult newts, known for their exceptional skin regeneration capabilities, are considered promising models for postnatal human studies. This study investigated actin fiber formation and alignment during re-epithelialization in the Japanese fire-bellied newt (Cynops pyrrhogaster). Methods: Full-thickness skin excisions were performed, and actin structures were analyzed using immunohistochemistry and electron microscopy. The role of actin in re-epithelialization was assessed by inhibiting its formation with cytochalasin B. Myosin, an interacting cytoskeletal molecule, was examined through immunohistochemistry, while E-cadherin, an adhesion molecule, was analyzed using both immunohistochemistry and electron microscopy. Results: Rather than an actin cable a mesh-like actin structure, termed the “actin mesh,” was identified via immunohistochemical analysis. The actin mesh developed alongside wound epidermis extension and disappeared following complete re-epithelialization. Inhibition of actin formation delayed re-epithelialization, although the overall healing process showed no significant difference from the control group. Immunohistochemistry revealed the presence of myosin II and E-cadherin alongside Filamentous actin. Electron microscopy further demonstrated actin-rich structures in the wound epidermis compared to normal skin and confirmed E-cadherin-mediated cell-cell adhesion in the wound area. Conclusions: The actin mesh plays a critical role in facilitating rapid re-epithelialization in adult newts, presenting a valuable model for studying scarless wound healing in adult organisms. The involvement of interacting molecules such as myosin and E-cadherin provides insights into the underlying mechanisms of this process. This model offers potential applications for addressing intractable wounds in humans.http://www.sciencedirect.com/science/article/pii/S2352320425000689RegenerationWound healingRe-epithelializationActinNewt |
| spellingShingle | Yu Liou Nobuko Moritoki Tomoko Shindo Tatsuyuki Ishii Kazuo Kishi Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) Regenerative Therapy Regeneration Wound healing Re-epithelialization Actin Newt |
| title | Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) |
| title_full | Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) |
| title_fullStr | Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) |
| title_full_unstemmed | Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) |
| title_short | Actin mesh in Re-epithelialization during skin regeneration in adult newt (Cynops pyrrhogaster) |
| title_sort | actin mesh in re epithelialization during skin regeneration in adult newt cynops pyrrhogaster |
| topic | Regeneration Wound healing Re-epithelialization Actin Newt |
| url | http://www.sciencedirect.com/science/article/pii/S2352320425000689 |
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