In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanizati...

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Main Authors: Fethi Ahmet Özdemir, Ömer Kılıç, Eyüp Bağcı
Format: Article
Language:English
Published: izzet kara 2017-11-01
Series:International Journal of Secondary Metabolite
Subjects:
Online Access:https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356264?publisher=ijate
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author Fethi Ahmet Özdemir
Ömer Kılıç
Eyüp Bağcı
author_facet Fethi Ahmet Özdemir
Ömer Kılıç
Eyüp Bağcı
author_sort Fethi Ahmet Özdemir
collection DOAJ
description Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted
format Article
id doaj-art-3c6da25ca23248febc9f6ecfc82ca6b0
institution DOAJ
issn 2148-6905
language English
publishDate 2017-11-01
publisher izzet kara
record_format Article
series International Journal of Secondary Metabolite
spelling doaj-art-3c6da25ca23248febc9f6ecfc82ca6b02025-08-20T02:39:11Zengizzet karaInternational Journal of Secondary Metabolite2148-69052017-11-0143, Special Issue 1505410.21448/ijsm.356264618In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) BakerFethi Ahmet ÖzdemirÖmer KılıçEyüp BağcıMuscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rootedhttps://dergipark.org.tr/tr/pub/ijsm/issue/32131/356264?publisher=ijatemuscari aucheriin vitrobulb regeneration
spellingShingle Fethi Ahmet Özdemir
Ömer Kılıç
Eyüp Bağcı
In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
International Journal of Secondary Metabolite
muscari aucheri
in vitro
bulb regeneration
title In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
title_full In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
title_fullStr In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
title_full_unstemmed In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
title_short In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
title_sort in vitro bulb regeneration from stem explants of endemic geophyt muscari aucheri boiss baker
topic muscari aucheri
in vitro
bulb regeneration
url https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356264?publisher=ijate
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AT omerkılıc invitrobulbregenerationfromstemexplantsofendemicgeophytmuscariaucheriboissbaker
AT eyupbagcı invitrobulbregenerationfromstemexplantsofendemicgeophytmuscariaucheriboissbaker