In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker
Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanizati...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
izzet kara
2017-11-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356264?publisher=ijate |
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| Summary: | Muscari aucheri (Boiss.) Baker bears beautiful and attractive
purplish blue flowers bloom between May and June; it grows stony slopes,
mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected
by increased environmental pollution, urbanization and grazing. Therefore, this
study aimed to accelerate multiplication by devising a strategy for an
efficient in vitro bulblet
regeneration system of Muscari aucheri
using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2,
0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet
regeneration on explants. Maximum mean number of bulblets per explant was noted
on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of
bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The
regenerated bulblets were isolated from stem explants and cultured on MS medium
containing 40 g/l sucrose; where they gained diameter and rooted |
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| ISSN: | 2148-6905 |