Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials
Background: Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purpl...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824001787 |
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| author | Ratri Maya Sitalaksmi Rizkipriyanto Azharpratomo Shafira Ninditya Irsan Primanda Nur Rahmania Sisca Meida Wati Syafira Salsabila Adam Kavanila Bilbalqish Khairul Anuar bin Shariff Harry Laksono Agus Dahlan Devi Rianti |
| author_facet | Ratri Maya Sitalaksmi Rizkipriyanto Azharpratomo Shafira Ninditya Irsan Primanda Nur Rahmania Sisca Meida Wati Syafira Salsabila Adam Kavanila Bilbalqish Khairul Anuar bin Shariff Harry Laksono Agus Dahlan Devi Rianti |
| author_sort | Ratri Maya Sitalaksmi |
| collection | DOAJ |
| description | Background: Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation. Aim: To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition. Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21. Results: The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21. Conclusion: The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21. |
| format | Article |
| id | doaj-art-3c60dc9417ca4350ab44c04f5e650d12 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-3c60dc9417ca4350ab44c04f5e650d122025-02-09T05:00:10ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-011516368Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterialsRatri Maya Sitalaksmi0Rizkipriyanto Azharpratomo1Shafira Ninditya Irsan2Primanda Nur Rahmania3Sisca Meida Wati4Syafira Salsabila Adam5Kavanila Bilbalqish6Khairul Anuar bin Shariff7Harry Laksono8Agus Dahlan9Devi Rianti10Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Indonesia; Corresponding author. Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No. 47 Surabaya, 60132, Indonesia.Resident of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaResident of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartment of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartment of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaFaculty of Dental Medicine, Universitas Airlangga, IndonesiaMagister Graduate Student of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaSchool of Material and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, MalaysiaDepartment of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartment of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, IndonesiaDepartment of dental materials, Faculty of Dentistry Medicine, Universitas Airlangga, IndonesiaBackground: Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation. Aim: To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition. Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21. Results: The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21. Conclusion: The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21.http://www.sciencedirect.com/science/article/pii/S2212426824001787Socket preservationHydroxyapatitePurple leavesBone regeneration |
| spellingShingle | Ratri Maya Sitalaksmi Rizkipriyanto Azharpratomo Shafira Ninditya Irsan Primanda Nur Rahmania Sisca Meida Wati Syafira Salsabila Adam Kavanila Bilbalqish Khairul Anuar bin Shariff Harry Laksono Agus Dahlan Devi Rianti Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials Journal of Oral Biology and Craniofacial Research Socket preservation Hydroxyapatite Purple leaves Bone regeneration |
| title | Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials |
| title_full | Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials |
| title_fullStr | Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials |
| title_full_unstemmed | Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials |
| title_short | Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials |
| title_sort | effectiveness of purple leaves graptophyllum pictum l griff and hydroxyapatite as socket preservation biomaterials |
| topic | Socket preservation Hydroxyapatite Purple leaves Bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824001787 |
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