Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II

Abstract Background Relative telomere length (rTL), a biomarker of biological ageing, has been implicated in type 2 diabetes and its complications. We aimed to identify the associates of rTL change over 4 years (∆rTL), and to investigate whether rTL and ∆rTL are associated with complications and mor...

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Main Authors: Chieh-Hsin Yang, Michael L H Huang, Wendy A Davis, Alicia J Jenkins, Timothy M E Davis
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Cardiovascular Diabetology
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Online Access:https://doi.org/10.1186/s12933-025-02832-3
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author Chieh-Hsin Yang
Michael L H Huang
Wendy A Davis
Alicia J Jenkins
Timothy M E Davis
author_facet Chieh-Hsin Yang
Michael L H Huang
Wendy A Davis
Alicia J Jenkins
Timothy M E Davis
author_sort Chieh-Hsin Yang
collection DOAJ
description Abstract Background Relative telomere length (rTL), a biomarker of biological ageing, has been implicated in type 2 diabetes and its complications. We aimed to identify the associates of rTL change over 4 years (∆rTL), and to investigate whether rTL and ∆rTL are associated with complications and mortality in adults with type 2 diabetes from the Australian observational community-based Fremantle Diabetes Study Phase II (FDS2). Methods Participants (n = 819) from the FDS2 cohort had baseline and Year-4 (mean ± SD 4.2 ± 0.4 years) rTL measured by qPCR (intra- and inter-assay %CV: 0.56% and 2.69%, respectively). The rTL change (∆rTL; % change/year) was categorised as Shortened (< − 2.69%), Unchanged (− 2.69% to + 2.69%) or Lengthened (> + 2.69%). Multiple logistic regression identified clinical and biochemical determinants of ∆rTL Shortened versus Not Shortened (Unchanged plus Lengthened). rTL and ∆rTL (continuous and categorical) were added to Cox and competing risk regression models of conventional predictors of major complications, CVD death and all-cause mortality during a mean ± SD 11.5 ± 2.1 years of follow-up. Results rTL was inversely correlated with age (r = − 0.186, P < 0.001). ∆rTL was shortened in 25.5% subjects, unchanged in 10.5%, and lengthened in 64.0%. Shortening was associated with older age, male sex, smoking, obesity, lipid-modifying drug use, and higher platelet count and serum bilirubin levels (P < 0.05). There were no statistically significant unadjusted or age- and sex-adjusted associations between baseline rTL, Year-4 rTL, or ∆rTL, and any incident micro- or macrovascular complications. In unadjusted Cox regression, ∆rTL lengthening was associated with a lower risk of CVD death (hazard ratio 0.98 (0.97, 0.99), P = 0.042) but this association became non-significant after adjustment for conventional risk factors. Conclusions In adults with type 2 diabetes, rTL does not always shorten over time. rTL and ∆rTL were associated with baseline conventional cardiometabolic risk factors but not independently with major incident complications. There was a weak association between ∆rTL and CVD mortality. These findings question the utility of rTL and ∆rTL in usual type 2 diabetes care.
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spelling doaj-art-3c59440109ec4d10b4fd41322ec7992e2025-08-20T03:37:19ZengBMCCardiovascular Diabetology1475-28402025-07-0124111310.1186/s12933-025-02832-3Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase IIChieh-Hsin Yang0Michael L H Huang1Wendy A Davis2Alicia J Jenkins3Timothy M E Davis4Baker Heart and Diabetes InstituteBaker Heart and Diabetes InstituteMedical School, University of Western Australia, Fremantle HospitalBaker Heart and Diabetes InstituteMedical School, University of Western Australia, Fremantle HospitalAbstract Background Relative telomere length (rTL), a biomarker of biological ageing, has been implicated in type 2 diabetes and its complications. We aimed to identify the associates of rTL change over 4 years (∆rTL), and to investigate whether rTL and ∆rTL are associated with complications and mortality in adults with type 2 diabetes from the Australian observational community-based Fremantle Diabetes Study Phase II (FDS2). Methods Participants (n = 819) from the FDS2 cohort had baseline and Year-4 (mean ± SD 4.2 ± 0.4 years) rTL measured by qPCR (intra- and inter-assay %CV: 0.56% and 2.69%, respectively). The rTL change (∆rTL; % change/year) was categorised as Shortened (< − 2.69%), Unchanged (− 2.69% to + 2.69%) or Lengthened (> + 2.69%). Multiple logistic regression identified clinical and biochemical determinants of ∆rTL Shortened versus Not Shortened (Unchanged plus Lengthened). rTL and ∆rTL (continuous and categorical) were added to Cox and competing risk regression models of conventional predictors of major complications, CVD death and all-cause mortality during a mean ± SD 11.5 ± 2.1 years of follow-up. Results rTL was inversely correlated with age (r = − 0.186, P < 0.001). ∆rTL was shortened in 25.5% subjects, unchanged in 10.5%, and lengthened in 64.0%. Shortening was associated with older age, male sex, smoking, obesity, lipid-modifying drug use, and higher platelet count and serum bilirubin levels (P < 0.05). There were no statistically significant unadjusted or age- and sex-adjusted associations between baseline rTL, Year-4 rTL, or ∆rTL, and any incident micro- or macrovascular complications. In unadjusted Cox regression, ∆rTL lengthening was associated with a lower risk of CVD death (hazard ratio 0.98 (0.97, 0.99), P = 0.042) but this association became non-significant after adjustment for conventional risk factors. Conclusions In adults with type 2 diabetes, rTL does not always shorten over time. rTL and ∆rTL were associated with baseline conventional cardiometabolic risk factors but not independently with major incident complications. There was a weak association between ∆rTL and CVD mortality. These findings question the utility of rTL and ∆rTL in usual type 2 diabetes care.https://doi.org/10.1186/s12933-025-02832-3Telomere lengthCardiovascular diseaseDiabetes complicationsType 2 diabetesMortality
spellingShingle Chieh-Hsin Yang
Michael L H Huang
Wendy A Davis
Alicia J Jenkins
Timothy M E Davis
Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
Cardiovascular Diabetology
Telomere length
Cardiovascular disease
Diabetes complications
Type 2 diabetes
Mortality
title Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
title_full Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
title_fullStr Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
title_full_unstemmed Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
title_short Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II
title_sort determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes the fremantle diabetes study phase ii
topic Telomere length
Cardiovascular disease
Diabetes complications
Type 2 diabetes
Mortality
url https://doi.org/10.1186/s12933-025-02832-3
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