Correlation between Biofilm Formation and Multi-Drug Resistance among Clinical Isolates
Introduction: Biofilms are often found in communities of microorganisms in chronic and persistent infections, exhibiting high resistance against antimicrobial agents. Biofilm serves as a barrier, impeding the penetration of drugs and constraining their effectiveness. Multiple methods, such as the...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-493-en.html |
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| Summary: | Introduction: Biofilms are often found in communities of microorganisms
in chronic and persistent infections, exhibiting high resistance against
antimicrobial agents. Biofilm serves as a barrier, impeding the penetration
of drugs and constraining their effectiveness. Multiple methods, such as the
Tissue Culture Plate method, Congo Red Agar method, Tube method,
bioluminescent assay, and fluorescent microscopic examination, can be used
to evaluate biofilm production. Methods: The study included a total of 300
clinical isolates representing a range of bacterial species, including
Acinetobacter baumannii (n=9), Coagulase Negative Staphylococcus (n=7),
Enterobacter aerogenes (n=7), Enterococcus faecalis (n=15), Escherichia
coli (n=137), Klebsiella pneumoniae (n=23), Proteus mirabilis (n=4),
Pseudomonas aeruginosa (n=16), Salmonella typhi (n=11), and
Staphylococcus aureus (n=68). Associations among isolates capable and
incapable of biofilm formation and their multidrug resistance phenotypes
were evaluated. Results: Among the 300 clinical isolates tested, 289 isolates
(96.3%) exhibited biofilm formation. The most prevalent biofilm-forming
organisms were A. baumannii (n=9), Citrobacter koseri (n=1), Coagulase
Negative Staphylococcus (CONS) (n=7), E. aerogenes (n=7), E. faecalis
(n=15), E. coli (n=137), Klebsiella oxytoca (n=1), K. pneumoniae (n=23),
P. mirabilis (n=4), P. aeruginosa (n=16), S. typhi (n=11), S. aureus (n=68),
and Streptococcus pneumoniae (n=1). The biofilm-forming isolates
demonstrated increased resistance compared to isolates that did not form
biofilms. Conclusion: Antimicrobial resistance represents a critical
characteristic of infections involving biofilms. The study identified biofilm
production in 92.7% of the isolates tested via TCP and in 72.3% of the
isolates using the CRA. Furthermore, it was observed that pathogens with
multidrug resistance (MDR) exhibited a higher biofilm production tendency
than non-producing pathogens. |
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| ISSN: | 2345-5349 2345-5330 |