Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression
Abstract Objective Resveratrol is a plant polyphenol known for its anti-inflammatory and pro-regenerative properties. These could be beneficial in controlling potential side effects of orthodontic treatment, such as apical root resorption. Orthodontic tooth movement occurs as part of a sterile infla...
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2025-02-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-05591-5 |
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| author | Shams Negm Michael Wolf Rogerio B. Craveiro Leon Schurgers Joachim Jankowski Rebekka K. Schneider Marta Rizk Franziska Coenen Isabel Knaup Sihem Brenji Christian Niederau |
| author_facet | Shams Negm Michael Wolf Rogerio B. Craveiro Leon Schurgers Joachim Jankowski Rebekka K. Schneider Marta Rizk Franziska Coenen Isabel Knaup Sihem Brenji Christian Niederau |
| author_sort | Shams Negm |
| collection | DOAJ |
| description | Abstract Objective Resveratrol is a plant polyphenol known for its anti-inflammatory and pro-regenerative properties. These could be beneficial in controlling potential side effects of orthodontic treatment, such as apical root resorption. Orthodontic tooth movement occurs as part of a sterile inflammatory response. However, dysregulation of this process can result in pathologically increased osteoclast activity in the radicular vicinity, leading to unwanted root resorption. Previous studies have shown that root cementum cells can modulate recruitment of osteoclast precursors and cementum repair. Material and methods We investigated the effect of resveratrol on mechanically stimulated murine cementoblasts (OCCM-30) with regards to cell viability, and mRNA expression and protein levels of pro-inflammatory cytokines. Furthermore, the modulation of central related kinases was investigated. Results Resveratrol increased viability of OCCM-30 in a time- and dose-dependent manner and significantly reduced upregulation of pERK and pAKT, upstream regulators of key cellular metabolic pathways. Furthermore, we describe for the first time that cementoblasts respond to compression with accelerated activation of STAT3 and increased translocation of NF-κB p65 into the nucleus. Conclusion This study shows a regulation of pAKT and pERK by resveratrol in OCCM-30 cells without a negative effect on cell viability. Therefore, resveratrol may have the potential to modulate the periodontal response to mechanical stimulation. |
| format | Article |
| id | doaj-art-3c4ba3d40d0f4d6db88b820bd44c41c9 |
| institution | DOAJ |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-3c4ba3d40d0f4d6db88b820bd44c41c92025-08-20T02:48:22ZengBMCBMC Oral Health1472-68312025-02-0125111210.1186/s12903-025-05591-5Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compressionShams Negm0Michael Wolf1Rogerio B. Craveiro2Leon Schurgers3Joachim Jankowski4Rebekka K. Schneider5Marta Rizk6Franziska Coenen7Isabel Knaup8Sihem Brenji9Christian Niederau10Department of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Biochemistry, CARIM, University of MaastrichtInstitute of Molecular Cardiovascular Research, Medical Faculty, RWTH Aachen UniversityDepartment of Cell and Tumor Biology, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityDepartment of Orthodontics, Medical Faculty, RWTH Aachen UniversityAbstract Objective Resveratrol is a plant polyphenol known for its anti-inflammatory and pro-regenerative properties. These could be beneficial in controlling potential side effects of orthodontic treatment, such as apical root resorption. Orthodontic tooth movement occurs as part of a sterile inflammatory response. However, dysregulation of this process can result in pathologically increased osteoclast activity in the radicular vicinity, leading to unwanted root resorption. Previous studies have shown that root cementum cells can modulate recruitment of osteoclast precursors and cementum repair. Material and methods We investigated the effect of resveratrol on mechanically stimulated murine cementoblasts (OCCM-30) with regards to cell viability, and mRNA expression and protein levels of pro-inflammatory cytokines. Furthermore, the modulation of central related kinases was investigated. Results Resveratrol increased viability of OCCM-30 in a time- and dose-dependent manner and significantly reduced upregulation of pERK and pAKT, upstream regulators of key cellular metabolic pathways. Furthermore, we describe for the first time that cementoblasts respond to compression with accelerated activation of STAT3 and increased translocation of NF-κB p65 into the nucleus. Conclusion This study shows a regulation of pAKT and pERK by resveratrol in OCCM-30 cells without a negative effect on cell viability. Therefore, resveratrol may have the potential to modulate the periodontal response to mechanical stimulation.https://doi.org/10.1186/s12903-025-05591-5OCCM-30Orthodontic tooth movementCompressionInflammationOrthodontically induced inflammatory root resorptionSterile inflammation |
| spellingShingle | Shams Negm Michael Wolf Rogerio B. Craveiro Leon Schurgers Joachim Jankowski Rebekka K. Schneider Marta Rizk Franziska Coenen Isabel Knaup Sihem Brenji Christian Niederau Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression BMC Oral Health OCCM-30 Orthodontic tooth movement Compression Inflammation Orthodontically induced inflammatory root resorption Sterile inflammation |
| title | Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression |
| title_full | Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression |
| title_fullStr | Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression |
| title_full_unstemmed | Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression |
| title_short | Resveratrol modulates phosphorylation of ERK and AKT in murine cementoblasts during in vitro orthodontic compression |
| title_sort | resveratrol modulates phosphorylation of erk and akt in murine cementoblasts during in vitro orthodontic compression |
| topic | OCCM-30 Orthodontic tooth movement Compression Inflammation Orthodontically induced inflammatory root resorption Sterile inflammation |
| url | https://doi.org/10.1186/s12903-025-05591-5 |
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