Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine
The pathogenesis of ulcerative colitis (UC) has been fundamentally associated with intestinal microbiota dysbiosis and disruption of immune homeostasis. This study systematically investigates the therapeutic potential of <i>Lactiplantibacillus plantarum</i> HYY-S10 (HYY-S10), a novel str...
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2025-04-01
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| author | Mengna Li Xintong Liu Weijian Chen Haoyue Xu Fang Huang Qingbo Yao Xiangze Jia Yanyan Huang |
| author_facet | Mengna Li Xintong Liu Weijian Chen Haoyue Xu Fang Huang Qingbo Yao Xiangze Jia Yanyan Huang |
| author_sort | Mengna Li |
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| description | The pathogenesis of ulcerative colitis (UC) has been fundamentally associated with intestinal microbiota dysbiosis and disruption of immune homeostasis. This study systematically investigates the therapeutic potential of <i>Lactiplantibacillus plantarum</i> HYY-S10 (HYY-S10), a novel strain isolated from De’ang sour tea in Yun an, China, with a focus on its mechanisms for alleviating colitis through the modulation of gut microbiota. Using a dextran sulfate sodium (DSS)-induced colitis model in C57BL/6J mice, our findings demonstrated that seven days of oral supplementation with HYY-S10 (1 × 10<sup>8</sup> CFU/mL, 0.2 mL/10 g body weight) significantly improved Disease Activity Index (DAI) scores and attenuated characteristic colitis symptoms, including progressive weight loss, rectal bleeding, and abnormal stool consistency. Administration of HYY-S10 exhibited significant immunomodulatory effects characterized by the downregulation of pro-inflammatory mediators (such as IL-1β, IL-6, IFN-γ, and LPS) while concomitantly upregulating anti-inflammatory IL-10 expression. Additionally, the strain enhanced intestinal antioxidant capacity by increasing GSH-Px activity, which collectively contributed to the reduction in intestinal inflammation. Furthermore, HYY-S10 demonstrated multifaceted protective effects by ameliorating oxidative stress through the restoration of redox homeostasis and modulation of gut microbial ecology. Probiotic intervention significantly increased short-chain fatty acids (SCFAs) production and notably enhanced the relative abundance of beneficial taxa, including <i>Akkermansia</i> and <i>Ruminococcus</i>_B, while restoring microbial diversity and ecological stability. Collectively, our results demonstrate that HYY-S10 alleviates experimental colitis by modulating the intestinal immune axis and microbiota composition, providing mechanistic insights to support its potential as a probiotic-based therapeutic strategy for UC. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Microorganisms |
| spelling | doaj-art-3c4b3a19c7ae4271b613fe42676ea7562025-08-20T03:13:58ZengMDPI AGMicroorganisms2076-26072025-04-0113484010.3390/microorganisms13040840Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the IntestineMengna Li0Xintong Liu1Weijian Chen2Haoyue Xu3Fang Huang4Qingbo Yao5Xiangze Jia6Yanyan Huang7Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaSchool of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, ChinaThe pathogenesis of ulcerative colitis (UC) has been fundamentally associated with intestinal microbiota dysbiosis and disruption of immune homeostasis. This study systematically investigates the therapeutic potential of <i>Lactiplantibacillus plantarum</i> HYY-S10 (HYY-S10), a novel strain isolated from De’ang sour tea in Yun an, China, with a focus on its mechanisms for alleviating colitis through the modulation of gut microbiota. Using a dextran sulfate sodium (DSS)-induced colitis model in C57BL/6J mice, our findings demonstrated that seven days of oral supplementation with HYY-S10 (1 × 10<sup>8</sup> CFU/mL, 0.2 mL/10 g body weight) significantly improved Disease Activity Index (DAI) scores and attenuated characteristic colitis symptoms, including progressive weight loss, rectal bleeding, and abnormal stool consistency. Administration of HYY-S10 exhibited significant immunomodulatory effects characterized by the downregulation of pro-inflammatory mediators (such as IL-1β, IL-6, IFN-γ, and LPS) while concomitantly upregulating anti-inflammatory IL-10 expression. Additionally, the strain enhanced intestinal antioxidant capacity by increasing GSH-Px activity, which collectively contributed to the reduction in intestinal inflammation. Furthermore, HYY-S10 demonstrated multifaceted protective effects by ameliorating oxidative stress through the restoration of redox homeostasis and modulation of gut microbial ecology. Probiotic intervention significantly increased short-chain fatty acids (SCFAs) production and notably enhanced the relative abundance of beneficial taxa, including <i>Akkermansia</i> and <i>Ruminococcus</i>_B, while restoring microbial diversity and ecological stability. Collectively, our results demonstrate that HYY-S10 alleviates experimental colitis by modulating the intestinal immune axis and microbiota composition, providing mechanistic insights to support its potential as a probiotic-based therapeutic strategy for UC.https://www.mdpi.com/2076-2607/13/4/840Yunnan De’ang Sour Teacolitis<i>Lactiplantibacillus plantarum</i> HYY-S10probioticsintestinal flora |
| spellingShingle | Mengna Li Xintong Liu Weijian Chen Haoyue Xu Fang Huang Qingbo Yao Xiangze Jia Yanyan Huang Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine Microorganisms Yunnan De’ang Sour Tea colitis <i>Lactiplantibacillus plantarum</i> HYY-S10 probiotics intestinal flora |
| title | Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine |
| title_full | Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine |
| title_fullStr | Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine |
| title_full_unstemmed | Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine |
| title_short | Alleviating Effect of <i>Lactiplantibacillus plantarum</i> HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine |
| title_sort | alleviating effect of i lactiplantibacillus plantarum i hyy s10 on colitis in mice based on an analysis of the immune axis in the intestine |
| topic | Yunnan De’ang Sour Tea colitis <i>Lactiplantibacillus plantarum</i> HYY-S10 probiotics intestinal flora |
| url | https://www.mdpi.com/2076-2607/13/4/840 |
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