Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities
Introduction: Plant-mediated green synthesis of nanoparticles has become a promising option in green nanotechnology because it is simple, cost-effective, eco-friendly, and biologically effective. This study focused on the synthesis and characterisation of silver nanoparticles (AgNPs) using Aidia d...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IIUM Press, International Islamic University Malaysia
2025-07-01
|
| Series: | Journal of Pharmacy |
| Subjects: | |
| Online Access: | https://journals.iium.edu.my/ktn/index.php/jp/article/view/390 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849411188032536576 |
|---|---|
| author | Ainul Hayati Zeheri Muhammad Taher Muhammad Taufiq Mohd Jailani Juliana Md Jaffri Deny Susanti Darnis Junaidi Khotib |
| author_facet | Ainul Hayati Zeheri Muhammad Taher Muhammad Taufiq Mohd Jailani Juliana Md Jaffri Deny Susanti Darnis Junaidi Khotib |
| author_sort | Ainul Hayati Zeheri |
| collection | DOAJ |
| description |
Introduction: Plant-mediated green synthesis of nanoparticles has become a promising option in green nanotechnology because it is simple, cost-effective, eco-friendly, and biologically effective. This study focused on the synthesis and characterisation of silver nanoparticles (AgNPs) using Aidia densiflora leaf extract, as well as the evaluation of their antimicrobial and cytotoxic activities. Methods: AgNPs were synthesised with A. densiflora leaf extract and their formation was confirmed using an ultraviolet–visible (UV–Vis) spectrophotometer. Liquid chromatography-mass spectrometry quadrupole time-of-flight was utilised for phytochemical profiling. The synthesised AgNPs were characterised using a zetasizer and zeta potential analyser, scanning electron microscopy-energy dispersive X-ray, Fourier Transform Infrared, X-ray diffraction (XRD), and thermogravimetric analysis. Antimicrobial activity of AD-AgNPs was tested against six microorganisms using the disc diffusion method, while cytotoxicity against MCF-7 human breast cancer cells was evaluated via MTT assay. Results: AgNP formation was confirmed by XRD and UV–Vis analysis, with absorbance peaks at 399–424 nm. Optimal synthesis was achieved using 10 mM AgNO? at 60°C and pH 7. SEM showed spherical-like nanoparticles averaging 96.06 nm with significant aggregation. The zeta potential was –35.6 mV, and XRD indicated a face-centred cubic structure with a crystalline size of 6.94 nm. AD-AgNPs showed no antimicrobial activity and low cytotoxicity. Conclusion: A. densiflora leaf extract can be used to synthesise AgNPs, however, further optimisation is required for better nanoparticle stabilisation and improvement of bioactivities.
|
| format | Article |
| id | doaj-art-3c463f40cc3143b5838edbaf8ed5d2b2 |
| institution | Kabale University |
| issn | 2773-5664 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | IIUM Press, International Islamic University Malaysia |
| record_format | Article |
| series | Journal of Pharmacy |
| spelling | doaj-art-3c463f40cc3143b5838edbaf8ed5d2b22025-08-20T03:34:52ZengIIUM Press, International Islamic University MalaysiaJournal of Pharmacy2773-56642025-07-0152Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and BioactivitiesAinul Hayati ZeheriMuhammad Taher0Muhammad Taufiq Mohd Jailani1Juliana Md Jaffri2Deny Susanti Darnis3Junaidi Khotib4IIUMIIUMIIUMIIUMUniversitas Airlangga Introduction: Plant-mediated green synthesis of nanoparticles has become a promising option in green nanotechnology because it is simple, cost-effective, eco-friendly, and biologically effective. This study focused on the synthesis and characterisation of silver nanoparticles (AgNPs) using Aidia densiflora leaf extract, as well as the evaluation of their antimicrobial and cytotoxic activities. Methods: AgNPs were synthesised with A. densiflora leaf extract and their formation was confirmed using an ultraviolet–visible (UV–Vis) spectrophotometer. Liquid chromatography-mass spectrometry quadrupole time-of-flight was utilised for phytochemical profiling. The synthesised AgNPs were characterised using a zetasizer and zeta potential analyser, scanning electron microscopy-energy dispersive X-ray, Fourier Transform Infrared, X-ray diffraction (XRD), and thermogravimetric analysis. Antimicrobial activity of AD-AgNPs was tested against six microorganisms using the disc diffusion method, while cytotoxicity against MCF-7 human breast cancer cells was evaluated via MTT assay. Results: AgNP formation was confirmed by XRD and UV–Vis analysis, with absorbance peaks at 399–424 nm. Optimal synthesis was achieved using 10 mM AgNO? at 60°C and pH 7. SEM showed spherical-like nanoparticles averaging 96.06 nm with significant aggregation. The zeta potential was –35.6 mV, and XRD indicated a face-centred cubic structure with a crystalline size of 6.94 nm. AD-AgNPs showed no antimicrobial activity and low cytotoxicity. Conclusion: A. densiflora leaf extract can be used to synthesise AgNPs, however, further optimisation is required for better nanoparticle stabilisation and improvement of bioactivities. https://journals.iium.edu.my/ktn/index.php/jp/article/view/390Metal nanoparticles, Aidia densiflora, Plant-mediated synthesis, Antimicrobial, Cytotoxicity |
| spellingShingle | Ainul Hayati Zeheri Muhammad Taher Muhammad Taufiq Mohd Jailani Juliana Md Jaffri Deny Susanti Darnis Junaidi Khotib Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities Journal of Pharmacy Metal nanoparticles, Aidia densiflora, Plant-mediated synthesis, Antimicrobial, Cytotoxicity |
| title | Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities |
| title_full | Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities |
| title_fullStr | Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities |
| title_full_unstemmed | Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities |
| title_short | Green Synthesis of Silver Nanoparticles Using Aidia densiflora Leaf Extract: Characterisation and Bioactivities |
| title_sort | green synthesis of silver nanoparticles using aidia densiflora leaf extract characterisation and bioactivities |
| topic | Metal nanoparticles, Aidia densiflora, Plant-mediated synthesis, Antimicrobial, Cytotoxicity |
| url | https://journals.iium.edu.my/ktn/index.php/jp/article/view/390 |
| work_keys_str_mv | AT ainulhayatizeheri greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities AT muhammadtaher greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities AT muhammadtaufiqmohdjailani greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities AT julianamdjaffri greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities AT denysusantidarnis greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities AT junaidikhotib greensynthesisofsilvernanoparticlesusingaidiadensifloraleafextractcharacterisationandbioactivities |