Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat

Rhizophora mangle, the red mangrove, has long been known as a traditional medicine. Its bark has been used as astringent, antiseptic, hemostatic, with antifungic and antiulcerogenic properties. In this paper, we aimed to evaluate the antioxidant properties of a buthanolic fraction of the R. mangle b...

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Main Authors: Felipe Meira de-Faria, Ana Cristina Alves Almeida, Anderson Luiz-Ferreira, Christiane Takayama, Ricardo José Dunder, Marcelo Aparecido da Silva, Marcos José Salvador, Patrícia Verardi Abdelnur, Marcos Nogueira Eberlin, Wagner Vilegas, Walber Toma, Alba Regina Monteiro Souza-Brito
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/327071
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author Felipe Meira de-Faria
Ana Cristina Alves Almeida
Anderson Luiz-Ferreira
Christiane Takayama
Ricardo José Dunder
Marcelo Aparecido da Silva
Marcos José Salvador
Patrícia Verardi Abdelnur
Marcos Nogueira Eberlin
Wagner Vilegas
Walber Toma
Alba Regina Monteiro Souza-Brito
author_facet Felipe Meira de-Faria
Ana Cristina Alves Almeida
Anderson Luiz-Ferreira
Christiane Takayama
Ricardo José Dunder
Marcelo Aparecido da Silva
Marcos José Salvador
Patrícia Verardi Abdelnur
Marcos Nogueira Eberlin
Wagner Vilegas
Walber Toma
Alba Regina Monteiro Souza-Brito
author_sort Felipe Meira de-Faria
collection DOAJ
description Rhizophora mangle, the red mangrove, has long been known as a traditional medicine. Its bark has been used as astringent, antiseptic, hemostatic, with antifungic and antiulcerogenic properties. In this paper, we aimed to evaluate the antioxidant properties of a buthanolic fraction of the R. mangle bark extract (RM) against experimental gastric ulcer in rats. Unib-Wh rats received pretreatment of R. mangle after the induction of gastric injury with absolute ethanol and ischemia-reperfusion. Gastric tissues from both methods were prepared to the enzymatic assays, the levels of sulfhydril compounds (GSH), lipid peroxides (LPO), and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The RM protected the gastric mucosa in both methods used, ethanol-induced gastric ulcer and ischemia-reperfusion, probably, by modulating the activities of the enzymes SOD, GPx, and GR and increasing or maintaining the levels of GSH; in adittion, LPO levels were reduced. The results suggest that the RM antioxidant activity leads to tissue protection; thus one of the antiulcer mechanisms present on the pharmacological effects of R. mangle is the antioxidant property.
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issn 1537-744X
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publishDate 2012-01-01
publisher Wiley
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spelling doaj-art-3c40c4d25d6b44389facd49d8f209d422025-08-20T02:08:19ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/327071327071Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the RatFelipe Meira de-Faria0Ana Cristina Alves Almeida1Anderson Luiz-Ferreira2Christiane Takayama3Ricardo José Dunder4Marcelo Aparecido da Silva5Marcos José Salvador6Patrícia Verardi Abdelnur7Marcos Nogueira Eberlin8Wagner Vilegas9Walber Toma10Alba Regina Monteiro Souza-Brito11Departamento de Farmacologia, Faculdade de Ciências Médicas, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Fisiologia e Biofísica, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Fisiologia e Biofísica, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Fisiologia e Biofísica, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Fisiologia e Biofísica, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Química Orgânica, Instituto de Química, UNESP, Araraquara, SP, BrazilDepartamento de Biologia Vegetal, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Química Orgânica, Instituto de Química, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Química Orgânica, Instituto de Química, UNICAMP, 13083-970 Campinas, SP, BrazilDepartamento de Química Orgânica, Instituto de Química, UNESP, Araraquara, SP, BrazilFaculdade de Farmácia, Universidade Santa Cecília, UNISANTA, Santos, SP, BrazilDepartamento de Farmacologia, Faculdade de Ciências Médicas, UNICAMP, 13083-970 Campinas, SP, BrazilRhizophora mangle, the red mangrove, has long been known as a traditional medicine. Its bark has been used as astringent, antiseptic, hemostatic, with antifungic and antiulcerogenic properties. In this paper, we aimed to evaluate the antioxidant properties of a buthanolic fraction of the R. mangle bark extract (RM) against experimental gastric ulcer in rats. Unib-Wh rats received pretreatment of R. mangle after the induction of gastric injury with absolute ethanol and ischemia-reperfusion. Gastric tissues from both methods were prepared to the enzymatic assays, the levels of sulfhydril compounds (GSH), lipid peroxides (LPO), and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The RM protected the gastric mucosa in both methods used, ethanol-induced gastric ulcer and ischemia-reperfusion, probably, by modulating the activities of the enzymes SOD, GPx, and GR and increasing or maintaining the levels of GSH; in adittion, LPO levels were reduced. The results suggest that the RM antioxidant activity leads to tissue protection; thus one of the antiulcer mechanisms present on the pharmacological effects of R. mangle is the antioxidant property.http://dx.doi.org/10.1100/2012/327071
spellingShingle Felipe Meira de-Faria
Ana Cristina Alves Almeida
Anderson Luiz-Ferreira
Christiane Takayama
Ricardo José Dunder
Marcelo Aparecido da Silva
Marcos José Salvador
Patrícia Verardi Abdelnur
Marcos Nogueira Eberlin
Wagner Vilegas
Walber Toma
Alba Regina Monteiro Souza-Brito
Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
The Scientific World Journal
title Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
title_full Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
title_fullStr Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
title_full_unstemmed Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
title_short Antioxidant Action of Mangrove Polyphenols against Gastric Damage Induced by Absolute Ethanol and Ischemia-Reperfusion in the Rat
title_sort antioxidant action of mangrove polyphenols against gastric damage induced by absolute ethanol and ischemia reperfusion in the rat
url http://dx.doi.org/10.1100/2012/327071
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