Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris
Abstract Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative approaches. This study demonstrates a signific...
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| Format: | Article |
| Language: | English |
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BMC
2025-03-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02683-3 |
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| author | Xiaocong Lu Mingxin Chang Xiangyu Li Wenbing Cao Zhoukang Zhuang Qian Wu Tao Yu Aiqun Yu Hongting Tang |
| author_facet | Xiaocong Lu Mingxin Chang Xiangyu Li Wenbing Cao Zhoukang Zhuang Qian Wu Tao Yu Aiqun Yu Hongting Tang |
| author_sort | Xiaocong Lu |
| collection | DOAJ |
| description | Abstract Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative approaches. This study demonstrates a significant enhancement in xylitol production using microbial cell factories, optimized through metabolic engineering. Two synthetic pathways were combined, and the introduction of a novel NADPH-dependent xylitol dehydrogenase further boosted xylitol yields, achieving 0.14 g xylitol/g glucose—a record-high yield for microbial systems. Additionally, the use of sustainable feedstocks, such as glycerol and methanol, led to the production of 7000 mg/L xylitol with a yield of 0.35 g xylitol/g glycerol, and 250 mg/L xylitol from methanol. These results underscore the potential for eco-friendly, cost-effective xylitol production, providing a robust foundation for future industrial-scale biotechnological applications. |
| format | Article |
| id | doaj-art-3c36b17202a849e4b6dbaca1d581d76b |
| institution | DOAJ |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-3c36b17202a849e4b6dbaca1d581d76b2025-08-20T02:56:20ZengBMCMicrobial Cell Factories1475-28592025-03-0124111110.1186/s12934-025-02683-3Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastorisXiaocong Lu0Mingxin Chang1Xiangyu Li2Wenbing Cao3Zhoukang Zhuang4Qian Wu5Tao Yu6Aiqun Yu7Hongting Tang8State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and TechnologyState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and TechnologyState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and TechnologyCenter for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes for Advanced Technology, Chinese Academy of SciencesCenter for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes for Advanced Technology, Chinese Academy of SciencesCenter for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes for Advanced Technology, Chinese Academy of SciencesCenter for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes for Advanced Technology, Chinese Academy of SciencesState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and TechnologySchool of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen UniversityAbstract Xylitol, known for its health benefits, is a valuable compound in the food and pharmaceutical industries. However, conventional chemical production methods are often unsustainable for large-scale applications, prompting the need for alternative approaches. This study demonstrates a significant enhancement in xylitol production using microbial cell factories, optimized through metabolic engineering. Two synthetic pathways were combined, and the introduction of a novel NADPH-dependent xylitol dehydrogenase further boosted xylitol yields, achieving 0.14 g xylitol/g glucose—a record-high yield for microbial systems. Additionally, the use of sustainable feedstocks, such as glycerol and methanol, led to the production of 7000 mg/L xylitol with a yield of 0.35 g xylitol/g glycerol, and 250 mg/L xylitol from methanol. These results underscore the potential for eco-friendly, cost-effective xylitol production, providing a robust foundation for future industrial-scale biotechnological applications.https://doi.org/10.1186/s12934-025-02683-3Metabolic engineeringXylitol biosynthesisSustainable carbon sourcesPichia pastoris |
| spellingShingle | Xiaocong Lu Mingxin Chang Xiangyu Li Wenbing Cao Zhoukang Zhuang Qian Wu Tao Yu Aiqun Yu Hongting Tang Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris Microbial Cell Factories Metabolic engineering Xylitol biosynthesis Sustainable carbon sources Pichia pastoris |
| title | Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris |
| title_full | Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris |
| title_fullStr | Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris |
| title_full_unstemmed | Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris |
| title_short | Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris |
| title_sort | metabolic engineering for sustainable xylitol production from diverse carbon sources in pichia pastoris |
| topic | Metabolic engineering Xylitol biosynthesis Sustainable carbon sources Pichia pastoris |
| url | https://doi.org/10.1186/s12934-025-02683-3 |
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