Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice
Envelope (env) proteins of certain endogenous retroviruses (ERVs) participate in various pathophysiological processes. In this study, we characterized pathophysiologic properties of two murine leukemia virus-type ERV (MuLV-ERV) env genes cloned from the ovary of C57BL/6J mice. The two env genes (nam...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/509604 |
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| author | Young-Kwan Lee Alex Chew David G. Greenhalgh Kiho Cho |
| author_facet | Young-Kwan Lee Alex Chew David G. Greenhalgh Kiho Cho |
| author_sort | Young-Kwan Lee |
| collection | DOAJ |
| description | Envelope (env) proteins of certain endogenous retroviruses (ERVs) participate in various pathophysiological processes. In this study, we characterized pathophysiologic properties of two murine leukemia virus-type ERV (MuLV-ERV) env genes cloned from the ovary of C57BL/6J mice. The two env genes (named ENVOV1 and ENVOV2), with 1,926\,bp coding region, originated from two MuLV-ERV loci on chromosomes 8 and 18, respectively. ENVOV1 and ENVOV2 were ~75 kDa and predominantly expressed on the cell membrane. They were capable of producing pseudotype murine leukemia virus virions. Tropism trait and infectivity of ENVOV2 were similar to the polytropic env; however, ENVOV1 had very low level of infectivity. Overexpression of ENVOV2, but not ENVOV1, exerted cytotoxic effects and induced expression of COX-2, IL-1β, IL-6, and iNOS. These findings suggest that the ENVOV1 and ENVOV2 are capable of serving as an env protein for virion assembly, and they exert differential cytotoxicity and modulation of inflammatory mediators. |
| format | Article |
| id | doaj-art-3c274fd0ed744eccafdd39fccbbde169 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3c274fd0ed744eccafdd39fccbbde1692025-08-20T02:08:16ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/509604509604Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J MiceYoung-Kwan Lee0Alex Chew1David G. Greenhalgh2Kiho Cho3Shriners Hospitals for Children Northern California and Department of Surgery, University of California, Davis, 2425 Stockton Boulevard, Sacramento, CA 95817, USAShriners Hospitals for Children Northern California and Department of Surgery, University of California, Davis, 2425 Stockton Boulevard, Sacramento, CA 95817, USAShriners Hospitals for Children Northern California and Department of Surgery, University of California, Davis, 2425 Stockton Boulevard, Sacramento, CA 95817, USAShriners Hospitals for Children Northern California and Department of Surgery, University of California, Davis, 2425 Stockton Boulevard, Sacramento, CA 95817, USAEnvelope (env) proteins of certain endogenous retroviruses (ERVs) participate in various pathophysiological processes. In this study, we characterized pathophysiologic properties of two murine leukemia virus-type ERV (MuLV-ERV) env genes cloned from the ovary of C57BL/6J mice. The two env genes (named ENVOV1 and ENVOV2), with 1,926\,bp coding region, originated from two MuLV-ERV loci on chromosomes 8 and 18, respectively. ENVOV1 and ENVOV2 were ~75 kDa and predominantly expressed on the cell membrane. They were capable of producing pseudotype murine leukemia virus virions. Tropism trait and infectivity of ENVOV2 were similar to the polytropic env; however, ENVOV1 had very low level of infectivity. Overexpression of ENVOV2, but not ENVOV1, exerted cytotoxic effects and induced expression of COX-2, IL-1β, IL-6, and iNOS. These findings suggest that the ENVOV1 and ENVOV2 are capable of serving as an env protein for virion assembly, and they exert differential cytotoxicity and modulation of inflammatory mediators.http://dx.doi.org/10.1155/2011/509604 |
| spellingShingle | Young-Kwan Lee Alex Chew David G. Greenhalgh Kiho Cho Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice Mediators of Inflammation |
| title | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice |
| title_full | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice |
| title_fullStr | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice |
| title_full_unstemmed | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice |
| title_short | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice |
| title_sort | tropism cytotoxicity and inflammatory properties of two envelope genes of murine leukemia virus type endogenous retroviruses of c57bl 6j mice |
| url | http://dx.doi.org/10.1155/2011/509604 |
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