Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities

Abstract Hyperthermophilic composting (HTC) system has attracted significant attention because of the extreme high temperature and special hyperthermophiles, but few researches were clearly explained how to achieve the hyperthermophilic conditions. Thereby, this study further analyzed the main facto...

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Main Authors: Zhiquan Wang, Zhe Chen, Shunfeng Jiang, Huachang Jin, Ruihuan Chen, Suqing Wu, Chunzhen Fan, Min Zhao, Xiaomei Gong, Minghai Xu, Xiangyong Zheng
Format: Article
Language:English
Published: SpringerOpen 2025-05-01
Series:Environmental Sciences Europe
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Online Access:https://doi.org/10.1186/s12302-025-01116-7
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author Zhiquan Wang
Zhe Chen
Shunfeng Jiang
Huachang Jin
Ruihuan Chen
Suqing Wu
Chunzhen Fan
Min Zhao
Xiaomei Gong
Minghai Xu
Xiangyong Zheng
author_facet Zhiquan Wang
Zhe Chen
Shunfeng Jiang
Huachang Jin
Ruihuan Chen
Suqing Wu
Chunzhen Fan
Min Zhao
Xiaomei Gong
Minghai Xu
Xiangyong Zheng
author_sort Zhiquan Wang
collection DOAJ
description Abstract Hyperthermophilic composting (HTC) system has attracted significant attention because of the extreme high temperature and special hyperthermophiles, but few researches were clearly explained how to achieve the hyperthermophilic conditions. Thereby, this study further analyzed the main factors affecting the metabolic heat accumulation based on the acclimated hyperthermophilic Bacillus thermoamylovorans. In total, our observations showed that the higher start-up temperature and easily degradable organic matter (OM) could promote the metabolic heat accumulation, the low OM and high heavy metal content had a negative effect on the rise of temperature. Especially, excessive easily degradable OM did not further increase the maximum temperature, but prolonged the thermophilic stage instead. Besides, the richness and diversity of bacteria were significantly decreased when the thermophilic stage stepped into hyperthermophilic stage, with the Sphingobacterium, Bacillus, and Lysinibacillus became the dominant bacterial communities. Among them, the acclimated Bacillus thermoamylovorans could account for 31% during the thermophilic stage, but rapidly decreased to 4% in hyperthermophilic stage. No strong dependence between rising temperature and diversity/richness of hyperthermophilic microbial communities was observed, which indicated that the existence of hyperthermophiles was one of necessary, but not sufficient conditions to achieve the hyperthermophilic conditions.
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spelling doaj-art-3c21302a89d041e59e564f845b277ee52025-08-20T02:34:14ZengSpringerOpenEnvironmental Sciences Europe2190-47152025-05-0137111210.1186/s12302-025-01116-7Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communitiesZhiquan Wang0Zhe Chen1Shunfeng Jiang2Huachang Jin3Ruihuan Chen4Suqing Wu5Chunzhen Fan6Min Zhao7Xiaomei Gong8Minghai Xu9Xiangyong Zheng10School of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversitySchool of Life and Environmental Science, Wenzhou UniversityShandong Yunmeng Environmental Protection Technology Co., LtdNatural Resources Monitoring Center of Pingyang CountySchool of Life and Environmental Science, Wenzhou UniversityAbstract Hyperthermophilic composting (HTC) system has attracted significant attention because of the extreme high temperature and special hyperthermophiles, but few researches were clearly explained how to achieve the hyperthermophilic conditions. Thereby, this study further analyzed the main factors affecting the metabolic heat accumulation based on the acclimated hyperthermophilic Bacillus thermoamylovorans. In total, our observations showed that the higher start-up temperature and easily degradable organic matter (OM) could promote the metabolic heat accumulation, the low OM and high heavy metal content had a negative effect on the rise of temperature. Especially, excessive easily degradable OM did not further increase the maximum temperature, but prolonged the thermophilic stage instead. Besides, the richness and diversity of bacteria were significantly decreased when the thermophilic stage stepped into hyperthermophilic stage, with the Sphingobacterium, Bacillus, and Lysinibacillus became the dominant bacterial communities. Among them, the acclimated Bacillus thermoamylovorans could account for 31% during the thermophilic stage, but rapidly decreased to 4% in hyperthermophilic stage. No strong dependence between rising temperature and diversity/richness of hyperthermophilic microbial communities was observed, which indicated that the existence of hyperthermophiles was one of necessary, but not sufficient conditions to achieve the hyperthermophilic conditions.https://doi.org/10.1186/s12302-025-01116-7Hyperthermophilic compostingHeat accumulationHyperthermophilesInfluencing factorsMicrobial communities
spellingShingle Zhiquan Wang
Zhe Chen
Shunfeng Jiang
Huachang Jin
Ruihuan Chen
Suqing Wu
Chunzhen Fan
Min Zhao
Xiaomei Gong
Minghai Xu
Xiangyong Zheng
Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
Environmental Sciences Europe
Hyperthermophilic composting
Heat accumulation
Hyperthermophiles
Influencing factors
Microbial communities
title Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
title_full Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
title_fullStr Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
title_full_unstemmed Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
title_short Improvement of metabolic heat accumulation for hyperthermophilic composting system: influencing factors and microbial communities
title_sort improvement of metabolic heat accumulation for hyperthermophilic composting system influencing factors and microbial communities
topic Hyperthermophilic composting
Heat accumulation
Hyperthermophiles
Influencing factors
Microbial communities
url https://doi.org/10.1186/s12302-025-01116-7
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