Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis
The CD30/CD30L signalling system has been implicated in the pathogenesis of several autoimmune and inflammatory conditions. In rheumatoid arthritis (RA), soluble CD30 (sCD30) levels reflect the recruitment of CD30+ T cells into the inflamed joints and correlate with a positive response to immunosupp...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/729654 |
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| author | Alessandro Barbieri Marzia Dolcino Elisa Tinazzi Antonella Rigo Giuseppe Argentino Giuseppe Patuzzo Andrea Ottria Ruggero Beri Antonio Puccetti Claudio Lunardi |
| author_facet | Alessandro Barbieri Marzia Dolcino Elisa Tinazzi Antonella Rigo Giuseppe Argentino Giuseppe Patuzzo Andrea Ottria Ruggero Beri Antonio Puccetti Claudio Lunardi |
| author_sort | Alessandro Barbieri |
| collection | DOAJ |
| description | The CD30/CD30L signalling system has been implicated in the pathogenesis of several autoimmune and inflammatory conditions. In rheumatoid arthritis (RA), soluble CD30 (sCD30) levels reflect the recruitment of CD30+ T cells into the inflamed joints and correlate with a positive response to immunosuppressive therapy. The aim of our report was to clarify the role of CD30/CD30L signalling system in the pathogenesis of RA. Our analysis of the CD30L+ T cell subsets in peripheral blood (PB) and synovial fluid (SF) of RA patients and of the related cytokine profiles suggests the involvement of CD30/CD30L signalling in polarization of T cells towards a Th17 phenotype with proinflammatory features. Moreover, in RA SF nearly 50% of Treg cells express CD30, probably as an attempt to downmodulate the ongoing inflammation. We also show here that the engagement of CD30L on neutrophils stimulated with CD30/Fc chimera may play a crucial role in RA inflammation since activated neutrophils release IL-8, thus potentially amplifying the local inflammatory damage. In conclusion, the results obtained suggest that the complex CD30/CD30L signalling pathway is implicated in the pathogenesis and progression of RA synovitis through a concerted action on several immune effector cells. |
| format | Article |
| id | doaj-art-3c20c86918a54522a83ca3d6e281ea5a |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-3c20c86918a54522a83ca3d6e281ea5a2025-08-20T02:08:16ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/729654729654Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid ArthritisAlessandro Barbieri0Marzia Dolcino1Elisa Tinazzi2Antonella Rigo3Giuseppe Argentino4Giuseppe Patuzzo5Andrea Ottria6Ruggero Beri7Antonio Puccetti8Claudio Lunardi9Department of Medicine, University of Verona, 37134 Verona, ItalyInstitute G. Gaslini, 16147 Genova, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyUniversity of Genova, 16132 Genova, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyInstitute G. Gaslini, 16147 Genova, ItalyDepartment of Medicine, University of Verona, 37134 Verona, ItalyThe CD30/CD30L signalling system has been implicated in the pathogenesis of several autoimmune and inflammatory conditions. In rheumatoid arthritis (RA), soluble CD30 (sCD30) levels reflect the recruitment of CD30+ T cells into the inflamed joints and correlate with a positive response to immunosuppressive therapy. The aim of our report was to clarify the role of CD30/CD30L signalling system in the pathogenesis of RA. Our analysis of the CD30L+ T cell subsets in peripheral blood (PB) and synovial fluid (SF) of RA patients and of the related cytokine profiles suggests the involvement of CD30/CD30L signalling in polarization of T cells towards a Th17 phenotype with proinflammatory features. Moreover, in RA SF nearly 50% of Treg cells express CD30, probably as an attempt to downmodulate the ongoing inflammation. We also show here that the engagement of CD30L on neutrophils stimulated with CD30/Fc chimera may play a crucial role in RA inflammation since activated neutrophils release IL-8, thus potentially amplifying the local inflammatory damage. In conclusion, the results obtained suggest that the complex CD30/CD30L signalling pathway is implicated in the pathogenesis and progression of RA synovitis through a concerted action on several immune effector cells.http://dx.doi.org/10.1155/2015/729654 |
| spellingShingle | Alessandro Barbieri Marzia Dolcino Elisa Tinazzi Antonella Rigo Giuseppe Argentino Giuseppe Patuzzo Andrea Ottria Ruggero Beri Antonio Puccetti Claudio Lunardi Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis Journal of Immunology Research |
| title | Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis |
| title_full | Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis |
| title_fullStr | Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis |
| title_full_unstemmed | Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis |
| title_short | Characterization of CD30/CD30L+ Cells in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis |
| title_sort | characterization of cd30 cd30l cells in peripheral blood and synovial fluid of patients with rheumatoid arthritis |
| url | http://dx.doi.org/10.1155/2015/729654 |
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