Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice
Objective A heavy infection in a primary molar tooth can impair the enamel formation of the underlying permanent successor. Helicobacter pylori colonizes primarily the stomach, but it has also been detected in oral samples, including in the dental pulp of infected primary teeth. Here, we aim to test...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2025.2500670 |
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| author | Carin Sahlberg Eija Salmela David P. Rice Kazuhiko Nakano Ryota Nomura Satu Alaluusua |
| author_facet | Carin Sahlberg Eija Salmela David P. Rice Kazuhiko Nakano Ryota Nomura Satu Alaluusua |
| author_sort | Carin Sahlberg |
| collection | DOAJ |
| description | Objective A heavy infection in a primary molar tooth can impair the enamel formation of the underlying permanent successor. Helicobacter pylori colonizes primarily the stomach, but it has also been detected in oral samples, including in the dental pulp of infected primary teeth. Here, we aim to test if H. pylori can disturb enamel and dentin formation.Methods Mandibular molar explants of E18.5 mice were grown for 12 days in media containing 10% of H. pylori cell lysates. The presence and extent of enamel and dentin on the mesial surface of the first molar explants were evaluated from stereomicroscopic photographs and histologically.Results The statistical analyses revealed that less enamel was formed in the test (N = 47) than in the control first molars (N = 28, p < 0.001). Most severe disturbances were seen in explants grown in media containing H. pylori cell lysates, which were made from stationary growth-phase cultures, with high optical density. Histological findings showed that dentin mineralization was also impaired.Conclusion The results suggest that H. pylori disturbs enamel and dentin development in cultured mouse embryonic molar teeth. This provides new insight into the etiology of enamel disturbances in permanent teeth. |
| format | Article |
| id | doaj-art-3c1d1abd90a74f9eadccbeb1e31fab46 |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-3c1d1abd90a74f9eadccbeb1e31fab462025-08-20T03:52:42ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2500670Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in miceCarin Sahlberg0Eija Salmela1David P. Rice2Kazuhiko Nakano3Ryota Nomura4Satu Alaluusua5Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, FinlandDepartment of Pediatric Dentistry, Graduate School of Dentistry, Osaka University, Osaka, JapanDepartment of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, JapanDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, FinlandObjective A heavy infection in a primary molar tooth can impair the enamel formation of the underlying permanent successor. Helicobacter pylori colonizes primarily the stomach, but it has also been detected in oral samples, including in the dental pulp of infected primary teeth. Here, we aim to test if H. pylori can disturb enamel and dentin formation.Methods Mandibular molar explants of E18.5 mice were grown for 12 days in media containing 10% of H. pylori cell lysates. The presence and extent of enamel and dentin on the mesial surface of the first molar explants were evaluated from stereomicroscopic photographs and histologically.Results The statistical analyses revealed that less enamel was formed in the test (N = 47) than in the control first molars (N = 28, p < 0.001). Most severe disturbances were seen in explants grown in media containing H. pylori cell lysates, which were made from stationary growth-phase cultures, with high optical density. Histological findings showed that dentin mineralization was also impaired.Conclusion The results suggest that H. pylori disturbs enamel and dentin development in cultured mouse embryonic molar teeth. This provides new insight into the etiology of enamel disturbances in permanent teeth.https://www.tandfonline.com/doi/10.1080/20002297.2025.2500670Helicobacter pyloridental enameldentinmouse molar teethgrowth phasesinfection |
| spellingShingle | Carin Sahlberg Eija Salmela David P. Rice Kazuhiko Nakano Ryota Nomura Satu Alaluusua Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice Journal of Oral Microbiology Helicobacter pylori dental enamel dentin mouse molar teeth growth phases infection |
| title | Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice |
| title_full | Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice |
| title_fullStr | Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice |
| title_full_unstemmed | Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice |
| title_short | Effect of Helicobacter pylori on enamel and dentin development – an in vitro study in mice |
| title_sort | effect of helicobacter pylori on enamel and dentin development an in vitro study in mice |
| topic | Helicobacter pylori dental enamel dentin mouse molar teeth growth phases infection |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2025.2500670 |
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