Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection
Abstract N 6 -methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified...
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2025-03-01
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| Online Access: | https://doi.org/10.1186/s13567-025-01495-y |
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| author | Zi-Han Wang Jing Li Sai-Ya Ma Meng-Xuan Liu Yu-Fei Zhan Feng Jin Bing-Xin Liu Wenjing Wang Mei He Yu-Chuan Yang Yandong Tang Peng Wang Wuchao Zhang Jie Tong |
| author_facet | Zi-Han Wang Jing Li Sai-Ya Ma Meng-Xuan Liu Yu-Fei Zhan Feng Jin Bing-Xin Liu Wenjing Wang Mei He Yu-Chuan Yang Yandong Tang Peng Wang Wuchao Zhang Jie Tong |
| author_sort | Zi-Han Wang |
| collection | DOAJ |
| description | Abstract N 6 -methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m6A-enriched peaks in PRRSV genomic RNA by m6A RNA immunoprecipitation sequencing (m6A-seq). Moreover, functional analyses revealed a positive correlation between the m6A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m6A-seq was also used to determine the m6A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m6A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m6A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics. |
| format | Article |
| id | doaj-art-3c1ca9a6f95049e8a07685be0657deb3 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-3c1ca9a6f95049e8a07685be0657deb32025-08-20T03:41:47ZengBMCVeterinary Research1297-97162025-03-015611910.1186/s13567-025-01495-yDynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infectionZi-Han Wang0Jing Li1Sai-Ya Ma2Meng-Xuan Liu3Yu-Fei Zhan4Feng Jin5Bing-Xin Liu6Wenjing Wang7Mei He8Yu-Chuan Yang9Yandong Tang10Peng Wang11Wuchao Zhang12Jie Tong13College of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityCollege of Veterinary Medicine, Hebei Agriculture UniversityState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural SciencesHebei Provincial Hospital of Traditional Chinese MedicineCollege of Veterinary Medicine, Hebei Agriculture UniversityCollege of Life Sciences, School of Life Sciences and Green Development, Hebei UniversityAbstract N 6 -methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m6A-enriched peaks in PRRSV genomic RNA by m6A RNA immunoprecipitation sequencing (m6A-seq). Moreover, functional analyses revealed a positive correlation between the m6A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m6A-seq was also used to determine the m6A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m6A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m6A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.https://doi.org/10.1186/s13567-025-01495-yN 6 -methyladenosine (m6a)porcine reproductive and respiratory syndrome virus (PRRSV)virus replicationm6A RNA immunoprecipitation sequencing (m6A-seq)p38/MAPK signalling pathway |
| spellingShingle | Zi-Han Wang Jing Li Sai-Ya Ma Meng-Xuan Liu Yu-Fei Zhan Feng Jin Bing-Xin Liu Wenjing Wang Mei He Yu-Chuan Yang Yandong Tang Peng Wang Wuchao Zhang Jie Tong Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection Veterinary Research N 6 -methyladenosine (m6a) porcine reproductive and respiratory syndrome virus (PRRSV) virus replication m6A RNA immunoprecipitation sequencing (m6A-seq) p38/MAPK signalling pathway |
| title | Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| title_full | Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| title_fullStr | Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| title_full_unstemmed | Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| title_short | Dynamic interplay between RNA N 6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| title_sort | dynamic interplay between rna n 6 methyladenosine modification and porcine reproductive and respiratory syndrome virus infection |
| topic | N 6 -methyladenosine (m6a) porcine reproductive and respiratory syndrome virus (PRRSV) virus replication m6A RNA immunoprecipitation sequencing (m6A-seq) p38/MAPK signalling pathway |
| url | https://doi.org/10.1186/s13567-025-01495-y |
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